关键词: 肾肿瘤；SPOP蛋白；A498细胞；泛素化；免疫组织化学法

Abstract:

Objective   To investigate the expression of SPOP in the renal clear cell cancer cell line, renal clear cell carcinoma and normal renal tissues, and to explore the relationship between SPOP expression and renal clear cell carcinoma. Methods   The expression of SPOP in renal clear cell cancer cell line A498 was detected by immunocytochemistry (DAKO EnVision system). Surgical biopsy specimens of renal clear cell carcinoma and the surrounding normal renal tissues were taken from the patients in the Department of Urology Surgery, the Second Hospital of Shandong University. Diagnosis of tumor pathological grades was carried out after HE staining. The expressions of SPOP in renal clear cell carcinoma and normal renal tissues were detected by immunohistochemistry (IHC, DAKO EnVision system) and Western blot. Results   SPOP was positively stained in the renal clear cell cancer cell line A498 by immunocytochemistry. Expression of SPOP was detected in renal clear cell carcinoma by IHC and Western blot, while SPOP in the normal renal tissues was uniformly negative. Conclusion   SPOP may be a new tumor marker of renal clear cell carcinoma. Surveillance of SPOP expression may be helpful to the diagnosis of renal clear cell carcinoma.

Key words: Renal neoplasms; Speckletype POZ protein; A498 cells; Ubiquitination; Immunohistochemistry