131I- rFliC的制备及乳腺癌荷瘤鼠体内生物学分布特征

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (12): 31-36.

131I- rFliC的制备及乳腺癌荷瘤鼠体内生物学分布特征

杨欢，梁婷，张超，宋静，郝静，侯桂华

山东大学医学院实验核医学研究所，济南 250012

收稿日期:2012-08-14 出版日期:2012-12-10 发布日期:2012-12-25
通讯作者: 侯桂华(1959- )，女，教授，博士生导师，主要从事实验核医学方面的研究。E-mail：ghhou@sdu.edu.cn
作者简介:杨欢（1988- ），女，硕士研究生，主要从事肿瘤核医学方面的研究。E-mail：huan0801@126.com
基金资助:
国家自然科学基金（81071172）；山东省自然科学基金（ZR2010CM025）

Preparation of 131I-rFliC and its biodistribution
in breast cancer-bearing mice

YANG Huan， LIANG Ting， ZHANG Chao， SONG Jing， HAO Jing， HOU Gui-hua

Institute of Experimental Nuclear Medicine， School of Medicine， Shandong University， Jinan 250012， China

Received:2012-08-14 Online:2012-12-10 Published:2012-12-25

摘要/Abstract

摘要：

目的探讨放射性碘131(131I)标记基因重组鞭毛蛋白（-rFliC ）在乳腺癌荷瘤小鼠体内的生物分布特征及意义。方法逆转录聚合酶链反应（RT-PCR）、蛋白质印迹法（Western blot）及免疫荧光检测人乳腺腺癌细胞系（MCF-7）中rFliC 的受体TLR5的表达；四氯二苯基苷脲（Iodogen）法131I标记rFliC, 检测其放射化学纯度、稳定性、细胞摄取状况；制备MCF-7荷瘤鼠，注射131I- rFliC后，观察其生物学分布及肿瘤靶向性。结果 ① MCF-7表达TLR5的mRNA及蛋白；随着rFliC浓度的增加，TLR5 mRNA及蛋白表达逐渐减弱；② 131I-rFliC标记率达95.19%；放射化学纯度为96.46%；在血清中稳定性高，可被MCF-7稳定摄取；③ 131I- iFliC 主要经肝肾代谢，肿瘤靶向性明显，24h 靶/肌肉（T/M）放射性比值为7.09，可获得清晰放射自显影像。结论 131I- rFliC肿瘤靶向明显，有望作为一种新型分子探针监测乳腺癌的发生发展。

关键词: 鞭毛蛋白；Toll样受体5；放射性碘标记；体内分布；乳腺肿瘤

Abstract:

Objective To investigate the biodistribution and significance of 131I- rFliC in breast cancer-bearing mice. Methods ① The expression of TLR5 was assayed by the immunofluorescence method，RT-PCR and Western blot. ② rFliC was labeled by 131I and the radiochemical purity, stability, cell uptake and efflux of 131I- rFliC were identified. ③ MCF-7 bearing mice were prepared and injected with 131I- rFliC. The biodistribution of 131I- rFliC and tumor targeting were analyzed. Results ① MCF-7 could express TLR5 at the mRNA and protein levels. The expression of TLR5 was down-regulated by rFliC with a dose-dependent manner. ② The labeling efficiency of 131I-rFliC was 95.19% and the radiochemical purity was 96.46%. 131I-rFliC was more stable in serum and could be absorbed by MCF-7 stably. ③ 24h after injection with 131I-rFliC, T/M ratio was 7.09, and tumor tissue appeared clearly with the autoradiography. Conclusion Tumor-targeting of 131I -rFliC is obvious, and it may become a new molecular probe for the diagnosis and therapy of breast cancer.

Key words: Flagellin; Toll-like Receptor 5; Radioactiveiodine; Biodistribution; Breast neoplasm

中图分类号:

R817.3

杨欢，梁婷，张超，宋静，郝静，侯桂华 . 131I- rFliC的制备及乳腺癌荷瘤鼠体内生物学分布特征[J]. 山东大学学报(医学版), 2012, 50(12): 31-36.

YANG Huan， LIANG Ting， ZHANG Chao， SONG Jing， HAO Jing， HOU Gui-hua. Preparation of 131I-rFliC and its biodistribution
in breast cancer-bearing mice[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(12): 31-36.

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