碱性成纤维细胞生长因子对动脉粥样硬化模型大鼠脑顶叶皮质与大脑中动脉的影响

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (11): 23-.

碱性成纤维细胞生长因子对动脉粥样硬化模型大鼠脑顶叶皮质与大脑中动脉的影响

朱俊德1, 2，王贵学2，余彦1，余资江1，肖朝伦1，王玉林1

1.贵阳医学院人体解剖学教研室，贵阳 550004； 2.教育部生物流变科学与技术重点实验室
重庆市血管植入工程实验室重庆大学生物工程学院，重庆 400044

收稿日期:2012-02-17 出版日期:2012-11-10 发布日期:2012-11-10
通讯作者: 王贵学(1963- )，男，博士研究生，博士生导师，主要从事血管生物力学与动脉粥样硬化发病机制及防治的研究。 E-mail：wanggx@cqu.edu.cn
作者简介:朱俊德(1973- )，男，硕士研究生，副教授,重庆大学访问学者，主要从事中枢神经损伤与修复的研究。
基金资助:
国家自然科学基金(30970721)；贵州省中医药管理局中医药、民族医药科学技术研究课题(QZYY201162);贵阳市科技局［(2010)黔科农合同字第1-社-11号］；贵阳医学院科学技术研究基金［合同字第(2009)31、56号］

Effects of basic fibroblast growth factor on cortex of parietal lobe and
middle cerebral artery in atherosclerotic model rats

ZHU Jun-de1,2, WANG Gui-xue2, YU Yan1, YU Zi-jiang1， XIAO Zhao-lun1， WANG Yu-lin1

1. Department of Anatomy, Guiyang Medical University, Guiyang, 550004, China；
2. Key Laboratory of Biorheological Science and Technology (Chongqing University),
Ministry of Education, Chongqing Engineering Laboratory in Vascular Implants,
Bioengineering College of Chongqing University, Chongqing 400044, China

Received:2012-02-17 Online:2012-11-10 Published:2012-11-10

摘要/Abstract

摘要：

目的探讨碱性成纤维细胞生长因子(bFGF)对动脉粥样硬化(AS)模型大鼠脑顶叶皮质与大脑中动脉M1段血管结构与功能的影响。方法随机将30只雄性Wistar大鼠分为正常对照组、AS组、bFGF治疗组。除正常对照组外在喂食开始时一次性腹腔注射维生素D3(6×104IU/kg)加高脂配方饲料喂养，6周后bFGF治疗组腹腔注射bFGF 9.5μg/kg，每日2次,连续14d。8周后处死大鼠，测各组血脂、脑组织生化指标、大脑中动脉壁胆固醇含量及Caspase-3蛋白表达水平；观察大脑中动脉M1段、脑顶叶皮质的病理改变。结果高脂饲料饲养6周后早期动脉粥样硬化形成，bFGF能降低血脂、血管壁胆固醇含量及Caspase-3蛋白表达水平(P<0.05)，降低脑组织丙二醛(MDA)含量，改善脑组织超氧化物歧化酶(SOD)和一氧化氮(NO)活性(P<0.05)；减轻AS所致大脑中动脉M1段与脑顶叶皮质的病理性损害(P<0.05)。结论 bFGF能改善AS模型大鼠的血脂与氧化能力、减轻脑组织与血管壁的病理损害，这些变化提示bFGF具有保护脑组织与脑血管的作用。

关键词: 成纤维细胞生长因子2；动脉硬化；动脉；神经元；大鼠

Abstract:

Objective To study the protective effects of basic fibroblast growth factor (bFGF) on cortex of parietal lobe and M1 segment of middle cerebral artery in atherosclerosis (AS) model rats. Methods A total of thirty male adult Wister rats were randomly divided into the normal control group, the AS model group and the bFGF treatment group. Except for the control group, rats of the other two groups were injected with a single dose of vitamin D3 (6×104IU/kg) and loaded with high fat diet for six weeks continuously. The bFGF was injection into the abdominal cavity after six weeks in the bFGF treatment group at 9.5μg/kg for two weeks continuously, and an identical volume saline was given to the AS model group and the normal control group. After eight weeks, all the rats were sacrificed. Then, the serum total cholesterol, triglyceride, low density lipoprotein and high density lipoprotein were respectively assayed, and the colorimetric method was used to detect the level of superoxide dismutase (SOD), Nitric-Oxide (NO) and the content of Malondialdehyde (MAD) in the brain tissues. The cholesterol and Caspase-3 content of the M1 segment of middle cerebral artery were respectively detected by the highperformance liquid chromatography and Western blot. As well the pathological lesion of the M1 segment of middle cerebral artery and cortex of parietal lobe were observed under a light microscopy and quantitative analysis was performed by cell morphometric technique. Results The early AS plaques were presented after six weeks by hyper lipid foods. Compared with those of the AS model group, the level of serum lipid, the content of cholesterol and Caspase-3 expression in the bFGF treatment group obviously decreased (P<0.05); The content of MAD and activity of SOD and NO also obviously decreased in the brain tissues (P<0.05); The pathologic injury of both the brain tissue and the M1 segment of middle cerebral artery decreased (P<0.05). Conclusion bFGF may play an important role in antagonizing the brain artery and cortex injuries not only by regulating the oxidizing ability and the level of blood lipid, but also by decreasing the pathologic injuries of both the brain tissue and artery.

Key words: Fibroblast growth factor2； Arteriosclerosis； Arteries； Neurons； Rats

中图分类号:

R743.3

朱俊德1, 2，王贵学2，余彦1，余资江1，肖朝伦1，王玉林1. 碱性成纤维细胞生长因子对动脉粥样硬化模型大鼠脑顶叶皮质与大脑中动脉的影响[J]. 山东大学学报(医学版), 2012, 50(11): 23-.

ZHU Jun-de1,2, WANG Gui-xue2, YU Yan1, YU Zi-jiang1， XIAO Zhao-lun1， WANG Yu-lin1 . Effects of basic fibroblast growth factor on cortex of parietal lobe and
middle cerebral artery in atherosclerotic model rats [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(11): 23-.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed

[1]	孙忠文1，杜鹃2，鲍梦馨3，陈娟1，朱梅佳4. 2型糖尿病大鼠脑组织TIPE2的表达特征及其与Aβ1-40沉积的相关性[J]. 山东大学学报(医学版), 2013, 51(3): 10-14.
[2]	张同霞1，焉传祝1，赵秀鹤1，迟令懿2. 大脑中动脉狭窄患者临床与神经影像学特点[J]. 山东大学学报(医学版), 2012, 50(11): 75-78.
[3]	胥文娟1，朱梅佳2，赵张宁2，孙忠文1，鲍梦馨1. 罗格列酮经LRP-1途径降低糖尿病大鼠脑微/小血管中β-淀粉样蛋白含量的研究[J]. 山东大学学报(医学版), 2012, 50(6): 5-.
[4]	李红军1，陈春富2. 奥扎格雷联合低分子肝素治疗进展性脑梗死的临床疗效[J]. 山东大学学报(医学版), 2011, 49(5): 119-.
[5]	杨健军1,3，杜怡峰2，韩晔3，胡浩然3. 脑局部亚低温治疗对急性脑梗死患者血清NSE和S-100蛋白的影响[J]. 山东大学学报(医学版), 2011, 49(4): 103-.
[6]	孙丽1,李云2,朱美蓉2,亓蕾2 . 对大鼠脑缺血再灌注后tTG表达的影响[J]. 山东大学学报(医学版), 2010, 48(7): 56-60.
[7]	邢月梅,曹枫林. 心理干预对预防卒中后抑郁发生的疗效观察[J]. 山东大学学报(医学版), 2010, 48(5): 161-162.
[8]	. 脉搏波传导速度与脑血管病的相关性[J]. 山东大学学报(医学版), 2009, 47(8): 97-99.