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山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (11): 18-.

• 基础医学 • 上一篇    下一篇

血管扩张刺激磷蛋白与微管蛋白的结合

陶燕   

  1. 江苏大学基础医学与医学技术学院, 江苏  镇江 212013
  • 收稿日期:2012-03-12 出版日期:2012-11-10 发布日期:2012-11-10
  • 作者简介:陶燕(1981- ),女,博士研究生,讲师,主要从事肿瘤分子诊断研究。
  • 基金资助:

    国家自然科学基金青年项目(31100974);江苏大学高级人才启动基金(11JDG032)

The combination between vasodilator-stimulated
phosphoprotein and microtubule protein

TAO Yan   

  1. School of Medical Science and Laboratory Medicine, Jiangsu University,  Zhenjiang 212013, Jiangsu, China
  • Received:2012-03-12 Online:2012-11-10 Published:2012-11-10

摘要:

目的   探讨血管扩张刺激磷蛋白(VASP)及其丝氨酸157(p-VASP S157)和239(p-VASP S239)位点磷酸化与α微管蛋白(α-Tubulin) 之间的关系,明确VASP是一个新的微管相关蛋白。方法   采用免疫荧光染色观察p-VASP S157、 p-VASP S239和α-Tubulin在人宫颈癌上皮细胞HeLa的共定位; 利用免疫共沉淀、微管沉淀和Western blot方法明确VASP及其不同位点磷酸化与α-Tubulin的结合情况。结果   VASP和p-VASP S157能够与αTubulin结合; p-VASP S239在细胞周期各个时期与α-Tubulin均无共定位关系。结论   VASP是一个新的微管相关蛋白,其丝氨酸157磷酸化与二者的结合相关。

关键词: 血管扩张刺激磷蛋白;磷酸化血管扩张刺激磷蛋白;α-微管蛋白;微管相关蛋白;人宫颈癌上皮细胞

Abstract:

Objective     To investigate the relationship between vasodilator-stimulated phosphoprotein (VASP), serine 157/serine 239 phosphorylated VASP (pVASP S157/p-VASP S239) and α-Tubulin, and to confirm VASP as a new microtubule-associated protein (MAP).  Methods    The co-localization of p-VASP S157/p-VASP S239 and α-Tubulin in Hela cells were analyzed by the immunofluorescencent assay. Co-immunoprecipitation assay, Microtubule pelleting assay and Western blot assay were applied to detect the combination between VASP/phosphor-VASP and α-Tubulin.  Results   VASP and p-VASP S157 could bind with α-Tubulin. p-VASP S239 did not co-localize with α-Tubulin during the whole cell cycle.  Conclusion    VASP is a new microtubule-associated protein, and the serine 157 phosphorylation of the protein is involved in the association between VASP and α-Tubulin.

Key words: Vasodilator-stimulated phosphoprotein; Phosphorylated vasodilator-stimulated phosphoprotein; αTubulin; Microtubule-associated protein;  Human cervix cancer cell line

中图分类号: 

  • R329.2+8
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