娃儿藤碱类化合物DCB-3503对TNBS诱导溃疡性结肠炎的治疗作用

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (10): 81-.

娃儿藤碱类化合物DCB-3503对TNBS诱导溃疡性结肠炎的治疗作用

孟宪邑，张芸，吴震州

南开大学生命科学学院, 天津 300071

收稿日期:2012-08-09 出版日期:2012-10-10 发布日期:2012-10-10
通讯作者: 吴震州（1979- ），男，讲师，主要从事基础免疫学研究。E-mail:wuzhenzhounk@163.com
作者简介:孟宪邑（1984- ），男，博士研究生，主要从事基础免疫学研究。

Therapeutic effect of tylophorine analog DCB3503 on
TNBS-induced ulcerative colitis

MENG Xian-yi, ZHANG Yun, WU Zhen-zhou

College of Life Science, Nankai University, Tianjin 300071, China

Received:2012-08-09 Online:2012-10-10 Published:2012-10-10

摘要/Abstract

摘要：

目的探讨娃儿藤碱类化合物DCB3503对三硝基苯磺酸(TNBS)诱导溃疡性结肠炎的治疗作用及可能的作用机制。方法选用C57BL/6野生型小鼠24只，分为对照(EtOH)组、实验(TNBS+DMSO)组、治疗(TNBS+DCB3503)组。造模3d后，观察各组间结肠长度、病理切片以及肠系膜淋巴结中IFN-γ+炎症性细胞和调节性T细胞(Treg)的差异。分离肠黏膜上皮细胞(IECs)总蛋白，Western blot检测Treg标志蛋白Foxp3的变化。结果治疗组损伤较实验组明显减轻，其肠系膜淋巴结中IFNγ+细胞显著减少，Treg细胞显著增多，且IECs中Foxp3表达明显上调。结论在溃疡性结肠炎中，DCB-3503通过诱导T细胞分化为Foxp3+Treg细胞，从而抑制IFN-γ+炎症性细胞发挥作用，最终缓解肠道的炎症反应。

关键词: 溃疡性结肠炎；三硝基苯磺酸；IFN-γ；Treg；Foxp3

Abstract:

Objective To evaluate the protective effect of tylophorine DCB-3503 on trinitrobenzene sulfonic acid (TNBS) induced ulcerative colitis and the possible molecular mechanism. Methods 24 C57BL/6 wild-type mice were divided into three groups: EtOH group, TNBS+DMSO group and TNBS+DCB-3503 group. Three days after the model was set up, the mice were killed and colon length, histological section, patterns of IFN-γ+ inflammatory cells and Treg cells were examined. Besides, the expression of Foxp3 in IECs was detected using Western blot. Results Compared with TNBS+DMSO group, DCB-3503 treatment group displayed remarkably improved clinical symptoms. Percentage of IFN-γ+ inflammatory cells in mesenteric lymph node was significantly decreased in TNBS+DCB-3503 group. Meanwhile the percentage of Treg cells in mesenteric lymph node and the expression of Foxp3 in intestinal epithelial cells (IECs) were significantly increased after DCB-3503 treatment. Conclusion The increase of Treg cells and decrease of IFN-γ+ inflammatory cells in DCB-3503 treatment group suggest that DCB-3503 can ameliorate the ulcerative colitis induced by TNBS via the growing number of Foxp3+ Treg cells and their suppression on IFN-γ+ inflammatory cells.

Key words: Ulcerative colitis; Trinitrobenzene sulfonic acid; IFN-γ; Treg; Foxp3

中图分类号:

R574.62

孟宪邑，张芸，吴震州. 娃儿藤碱类化合物DCB-3503对TNBS诱导溃疡性结肠炎的治疗作用[J]. 山东大学学报(医学版), 2012, 50(10): 81-.

MENG Xian-yi, ZHANG Yun, WU Zhen-zhou. Therapeutic effect of tylophorine analog DCB3503 on
TNBS-induced ulcerative colitis[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(10): 81-.

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