关键词: 壳聚糖磷脂酰胆碱; 阿尔茨海默病;淀粉样β蛋白; 蛋白激酶C; 钙结合蛋白质类

Abstract:

Objective   To investigate the effect of chitosan phosphatidyl choline on the expressions of protein kinase C-δ(PKC-δ)and calcium binding protein( Iba1)in AD rats brain induced by Aβ25-35. Methods   Fifty wistar rats were randomly divided into five groups: the control group， the AD model group，the chitosan phosphatidyl choline low-dose group, the medium-dose group and the high-dose group. Two point five microliter of Aβ25-35(5μg/μL) was injected into the CA1 area of the rat hippocampus to establish the AD model. The rat learning and memory abilities were tested through the method of Morris water maze. The expressions of PKC-δ and Iba1 in hippocampus and cerebral cortex were detected with the immunohistochemical method. Results   Chitosan phosphatidyl choline could obviously shorten the AD rats latency caused by Aβ2535, increase the times of passing through platform，and significantly reduce the  PKC-δ and Iba1 positive expressions in AD hippocampus and cerebral cortex of rats. Conclusion   Chitosan phosphatidyl choline can improve the learning and memory abilities of AD rats caused by Aβ25-35, depress the proliferation and activation of microglia in the rat brain, lessen inflammation response ，and contribute to neuroprotection and anti-dementia through down-regulating the PKC-δ expression.

Key words: Chitosan phosphatidyl choline; Alzheimer disease; Amyloid beta-protein; Proteinase C; Calciumbinding protein