染料介导光氧化及京尼平交联处理的同种异体动脉性能研究

山东大学学报(医学版) ›› 2012, Vol. 50 ›› Issue (10): 41-.

染料介导光氧化及京尼平交联处理的同种异体动脉性能研究

张冬，张军，刘鲁祁

山东大学附属千佛山医院心脏外科，济南 250014

收稿日期:2011-12-09 出版日期:2012-10-10 发布日期:2012-10-10
通讯作者: 刘鲁祁（1960- ），男，博士生导师, 主要从事心脏病的外科治疗研究。 E-mail:luqizh@163.com
作者简介:张冬（1986- ），男，硕士研究生, 主要从事心脏外科研究
基金资助:
山东省自然科学基金（Y2007C011）

Research on the properties of allogenic arteries cross-linked by
dye-mediated photooxidation and Genipin

ZHANG Dong, ZHANG Jun, LIU Lu-qi

Department of Cardiovascular Surgery, Qianfoshan Hospital Affiliated to Shandong University, Jinan 250014, China

Received:2011-12-09 Online:2012-10-10 Published:2012-10-10

摘要/Abstract

摘要：

目的利用不同交联方法处理的生物组织具有不同生物学特性，观察染料介导光氧化(DMP)及京尼平(GP)交联后的同种异体动脉的物理特性及生物相容性。方法以新西兰兔胸主动脉(血管内径4mm左右)为研究对象，按对其交联处理方法的不同分为DMP交联处理组(DMP组)、GP交联处理组(GP组)、戊二醛(GA)交联处理组(GA组)，同时设立实验对照组(即未进行任何特殊交联处理)。经不同交联处理后，测定各组的组织含水量、热皱缩温度、断裂强度，并进行体外溶血试验，观察处理后动脉的生物相容性。结果形态学观察：GP组与GA组透光度减低，弹性、韧性明显增强，DMP组透光度较高，弹性、韧性未见增强；组织含水量：对照组＞DMP组＞GP组＞GA组；热皱缩温度：GA组＞GP组＞DMP组＞对照组；断裂强度：GA组＞GP组＞DMP组＞对照组；体外溶血指数：GA组＞GP组＞DMP组。结论染料介导光氧化及京尼平交联处理的血管材料显示出良好的物理特性和生物相容性，适宜塑型及体内植入。

关键词: 染料介导光氧化DMP；京尼平GP；同种异体动脉；交联处理

Abstract:

Objective To detect the physical characteristics and the biocompatibility of allogenic arteries cross-linked by dye-mediated photooxidation (DMP) and Genipin (GP) based on the former researches that biological tissue which have gone through different cross-linked methods will show up different biological properties. Methods The thoracic arteries of New Zealand rabbits (with the inside vessel diameter of 4mm), according to different cross-linked methods, were divided into the DMP group, the GP group, the Glutaraldehyde(GA) group, and a control group was also established (which had not gone through any crosslinked treatment). After the arteries were cross-linked with different methods, tissue water content, heat shrinking temperature, breaking tenacity, and biocompatibility which had gone through the external hemolytic test were detected. Results Morphological observation: The transmittance of the GP group and the GA group was reduced, while the elasticity and toughness were obviously enhanced. The transmittance of the DMP group was superior, while the elasticity and toughness were not enhanced; Tissue water content: control group>DMP group>GP group>GA group; Heat shrinking temperature: GA group>GP group>DMP group>control group; Breaking tenacity: GA group>GP group>DMP group>control group; Indices of external hemolytic: GA group>GP group>DMP group. Conclusions Vascular materials cross-linked by dye-mediated photooxidation and Genipin have shown better physical characteristics and biocompatibility. So it is suitable for molding and implanting in human body.

Key words: Dye-mediated photooxidation; Genipin; Allogenic artery; Cross-linked

中图分类号:

R654.3

张冬，张军，刘鲁祁. 染料介导光氧化及京尼平交联处理的同种异体动脉性能研究[J]. 山东大学学报(医学版), 2012, 50(10): 41-.

ZHANG Dong, ZHANG Jun, LIU Lu-qi. Research on the properties of allogenic arteries cross-linked by
dye-mediated photooxidation and Genipin[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2012, 50(10): 41-.

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