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山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (8): 67-.

• 论文 • 上一篇    下一篇

压力对猪眼小梁细胞表达ELAM-1、MMPs/TIMPs的影响

朱玉广,王杰,范晓军,朱艳,钟莹莹,杜孝楠   

  1. 潍坊医学院附属医院眼科中心,  山东 潍坊 261031
  • 收稿日期:2011-02-18 出版日期:2011-08-10 发布日期:2011-08-10
  • 作者简介:朱玉广(1972- ),男,博士,副教授,主要从事青光眼与白内障的研究。 E-mail:yg-zhu.md@tom.com
  • 基金资助:

    山东省自然科学基金资助项目(Y2008C127);山东省教育厅科技计划资助项目(J08LG13)。

Effect of pressure on expressions of ELAM-1, MMP-2,MMP-3 and  TIMP-2 in porcine trabecular meshwork cells cultured in vitro

ZHU Yuguang, WANG Jie, FAN Xiaojun, ZHU Yan, ZHONG Yingying, DU Xiaonan   

  1. Eye Center, Affiliated Hospital of Weifang Medical College, Weifang 261031, Shandong,  China
  • Received:2011-02-18 Online:2011-08-10 Published:2011-08-10

摘要:

目的     培养猪眼小梁细胞,研究压力对体外培养的猪眼小梁内皮细胞白细胞粘附分子-1 (ELAM-1)、基质金属蛋白酶MMP-2、MMP-3及基质金属蛋白酶组织抑制剂TIMP-2表达的影响。方法      培养猪眼小梁细胞并鉴定,建立细胞水平的青光眼模型,对传第3代猪眼小梁细胞分别施加20、40、60 、80mmHg压力作为实验组,0mmHg为对照组。培养6h后行ELAM-1免疫组织化学SP法染色,培养24h后行MMP-2、MMP-3和TIMP-2免疫组织化学SP法染色,并对染色结果进行统计学分析。结果      培养细胞为猪眼小梁细胞,正常小梁细胞不表达ELAM-1,压力为40、60、80mmHg时,ELAM-1的表达同0、20mmHg组相比表达明显增加。正常小梁细胞可以少量表达MMP-2、MMP-3及TIMP-2。压力为40、60mmHg时,MMP-2、TIMP-2的表达同0、20、80mmHg相比表达明显增加,压力不影响小梁细胞MMP-3的表达。结论      一定范围内压力的变化可以促进猪眼小梁细胞表达ELAM-1,可以改变MMPs/TIMPs之间的平衡状态,进而影响小梁细胞外基质(ECM)的代谢,改变房水外流阻力, ELAM-1、MMPs在青光眼的发病中可能发挥重要作用。

关键词: 压力;小梁细胞;内皮细胞白细胞黏附分子-1;基质金属蛋白酶

Abstract:

Objective      To investigate the effect of pressure on expressions of endothelial leukocyte adhesion molecule-1(ELAM-1), matrix metalloproteinase-2 (MMP-2), MMP-3 and Tissue Inhibitor of Metalloproteinase-2(TIMP-2) in porcine trabecular cells cultured in vitro.  Methods     Porcine trabecular cells were cultured, passaged and identified. The third passage generation trabecular cells were treated under pressure of 20, 40, 60 and 80mmHg(1kPa=7.5mmHg) in the treatment group to establish a cell-level glaucoma model, while no pressure(0mmHg) was given to the control group. Immunocytochemical staining was used to observe expression of ELAM-1 after 6 hours’culture, and expressions of MMP-2, MMP-3 and TIMP-2 after 24 hours’culture. Staining results were statistically analyzed. Results    The cultured cells were identitied as porcine trabecular cells. Expression of ELAM-1 was not detectd in trabecular cells of the control group,while expressions of MMP-2, MMP-3 and TIMP-2 were observed in small quantity. When pressure was 40, 60 or 80mmHg, expression of ELAM-1 significantly increased compared with the 0mmHg group and 20mmHg group. When pressure was 40 or 60mmHg, expressions of MMP-2 and TIMP-2 significantly increased compared with the 0mmHg group, 20mmHg group and 80mmHg group. Expression of MMP-3 had no significant variation. Conclusion     A rise of pressure can induce an increase in expressions of ELAM-1, MMP-2 and TIMP-1 in cultured porcine trabecular cells, causes unbalance of MMPs/TIMPs, affects metabolism of extracellularmatrix(ECM) and increasesaqueous outflow. Expressions of ELAM-1 and MMPs may contribute to pathogenesis of glaucoma.

Key words: Pressure; Trabecular cells; Endothelial leukocyte adhesion molecule-1; Matrix metalloproteinases

中图分类号: 

  • R775.9
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