应用噬菌体随机十二肽库筛选TGF-β1相关模拟肽的实验研究

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (8): 70-73.

应用噬菌体随机十二肽库筛选TGF-β1相关模拟肽的实验研究

刘振中1，姜笃银1,2，蔡景龙3，宗宪磊3，张基勋1，单菲1,2，王文婷1,2，王魏1,2

1.山东大学第二医院美容整形烧伤外科，济南 250033； 2.山东大学第二医院组织工程研究所，济南 250033；
3.中国医学科学院整形外科医院十六科，北京100144

收稿日期:2011-03-29 出版日期:2011-08-10 发布日期:2011-08-10
通讯作者: 姜笃银(1963- )，男，教授，博士生导师，主要从事创伤修复、组织工程材料基础和临床应用研究。 E-mail: jdybs2 @vip.163.com
作者简介:刘振中(1975- )，男，主治医师，博士研究生，主要从事整形与美容外科及瘢痕防治研究。
基金资助:
国家自然科学基金资助课题(30670571，30772258，81071560)；山东省自然科学基金资助课题(ZR2010HQ010)；山东省科技攻关项目资助课题(2009GG10002078)。

TGF-β1-related model peptides isolated from a phage display 12-mer peptide library

LIU Zhenzhong1, JIANG Duyin1,2, CAI Jinglong3, ZONG Xianlei3, ZHANG Jixun1, SHAN Fei1,2, WANG Wenting1,2, WANG Wei1,2

1. Department of Plastic Surgery, The Second Hospital of Shandong University, Jinan 250033, China;
2. Institute of Tissue Engineering, The Second Hospital of Shandong University, Jinan 250033, China;
3 Sixteenth Department of Plastic Surgery Hospital, Chinese Academy of Medical Sciences, Beijing 100144, China

Received:2011-03-29 Online:2011-08-10 Published:2011-08-10

摘要/Abstract

摘要：

目的应用噬菌体随机12肽库生物筛选获得转化生长因子-β1(TGF-β1)噬菌体模拟肽。方法以人TGF-β1单克隆抗体为靶，进行4轮生物淘选，随机挑取单克隆噬菌体，ELISA法检测单克隆噬菌体的结合力。对结合力较好的单克隆噬菌体提取噬菌体DNA，进行测序，行序列分析。结果经过4 轮生物淘选，特异性噬菌体模拟肽获得了富集，ELISA 检测结果显示，获得的一些噬菌体模拟肽具有较好的结合力，测序获得10个与促进成纤维细胞增殖或抑制细胞增殖有关的碱基序列。结论从噬菌体随机十二肽库中可淘选到与TGF-β1相关的噬菌体模拟肽。

关键词: 噬菌体随机12肽库；转化生长因子β1噬菌体模拟肽；生物淘选；测序

Abstract:

Objective To harvest TGF-β1 model peptides from a phage display 12-mer peptide library. Methods A phage display 12-mer peptide library was screened 4 times by the monoclonal anti-human TGF-β1. Monoclonal phages were randomly selected by ELISA. DNA extracted from phages with better binding activity was sequenced and analyzed. Results After 4 times of screening, abundant specific phage model peptides were harvested. The results of ELISA showed that some phage model peptides had a fine binding activity. A total of 10 sequences related to promoting or inhibiting fibroblast cell proliferation were recognized. Conclusion TGF-β1-related phage model peptides can be harvested from a phage display 12-mer peptide library, and some phage model peptides may inhibit keloid fibroblast proliferation.

Key words: Phage display 12-mer peptide library; TGF-β1 phage model peptides; Biological screening; Sequencing

中图分类号:

R622

刘振中1，姜笃银1,2，蔡景龙3，宗宪磊3，张基勋1，单菲1,2，王文婷1,2，王魏1,2. 应用噬菌体随机十二肽库筛选TGF-β1相关模拟肽的实验研究[J]. 山东大学学报(医学版), 2011, 49(8): 70-73.

LIU Zhenzhong1, JIANG Duyin1,2, CAI Jinglong3, ZONG Xianlei3, ZHANG Jixun1, SHAN Fei1,2, WANG Wenting1,2, WANG Wei1,2. TGF-β1-related model peptides isolated from a phage display 12-mer peptide library[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(8): 70-73.

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