不同中波紫外线对豚鼠皮肤色素沉着及α-黑素细胞刺激素表达的影响

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (3): 59-63.

不同中波紫外线对豚鼠皮肤色素沉着及α-黑素细胞刺激素表达的影响

张敏1,2,李春阳2, 王克玉2, 杨秀莉1

山东大学 1.附属济南市中心医院皮肤科，济南 250013； 2.齐鲁医院皮肤科，济南 250012

收稿日期:2010-08-04 出版日期:2011-03-10 发布日期:2011-03-10
通讯作者: 李春阳（1949- ），女，教授，博士生导师，研究方向为皮肤性病与真菌病。 E-mail：lichunyang@medmail.com.cn
作者简介:张敏（1976- ），女，博士研究生，主治医师，研究方向为色素障碍性皮肤病。 E-mail：zm4602@126.com

Effects of ultraviolet irradiation at different wavelengths on the melanin level and expression of α-melanocyte-stimulating hormone in the skin of guinea pigs

ZHANG Min1,2， LI Chun-yang2， WANG Ke-yu2， YANG Xiu-li1

1. Department of Dermatology, Jinan Central Hospital Affiliated to Shandong University, Jinan 250013, China;
2. Department of Dermatology, Qilu Hospital of Shandong University, Jinan 250012, China

Received:2010-08-04 Online:2011-03-10 Published:2011-03-10

摘要/Abstract

摘要：

目的探讨不同剂量定向中波高能紫外线及311nm窄谱中波紫外线（NB-UVB）照射对豚鼠皮肤色素沉着及表达α黑素细胞刺激素（αMSH）的影响。方法以正常棕黄色豚鼠为实验模型，分为定向中波高能紫外线高、低剂量组，NB-UVB高、低剂量组及空白对照组进行照射，分别采用肉眼评估及黑素颗粒染色(FontanaMasson法)研究其致色素沉着作用；通过免疫组化法检测豚鼠皮肤中的α-MSH表达情况。结果各组的色素沉着评分、黑素颗粒含量差异均有统计学意义(P<0.01)，定向中波高能紫外线高、低剂量组之间差异无统计学意义（P>0.05），但均高于NBUVB组（P<0.05），且NB-UVB高剂量组高于低剂量组（P<0.05）；各组的α-MSH免疫组化计分差异有统计学意义(P<0.01)，定向中波高能紫外线组高于NBUVB组（P<0.01），但每种光源不同剂量组之间差异无统计学意义（P>0.05）。结论定向中波高能紫外线在致色素沉着作用方面优于NB-UVB；两种紫外线均可促进表皮中α-MSH的表达，且定向中波高能紫外线作用更为显著。

关键词: 紫外线；豚鼠；角质形成细胞；色素沉着；α-黑素细胞刺激素

Abstract:

Objective To study effects of various dosages of targeted high-intensity UVB and 311nm narrow band UVB (NB-UVB) on hyperpigmentation and expression of αmelanocyte-stimulating hormone(α-MSH) in the skin of guinea pigs. Methods Five isolated areas were selected on the skin of brownish guinea pigs, and treated with different dosages of targeted high-intensity UVB and NB-UVB. Hyperpigmentation was evaluated by visual assessment and Fontana-Masson staining, and expression of αMSH was determined by immunohistochemistry. Results Hyperpigmentation scores and melanin contents among the 5 groups were significantly different(P<0.01). There was no statistically significant difference between the high dosage and low dosage groups of targeted high-intensity UVB(P>0.05). However, they were significantly higher in targeted high-intensity UVB groups than in NB-UVB groups (P<0.05), and significantly higher in the high dosage group than in the low dosage group of NB-UVB（P<0.05）. Immunohistochemical scores of α-MSH among the 5 groups were significantly different(P<0.01)， and they were significantly higher in targeted high-intensity UVB groups than in NB-UVB groups（P<0.01）. However, there was no statistically significant difference between the high dosage and low dosage groups of each wavelength (P>0.05). Conclusions Targeted high-intensity UVB phototherapy is more effective than NB-UVB to induce experimental hyper-pigmentation. Each kindof ultraviolet light can up-regulate expression of α-MSH in the epidermis, and the effect of targeted high-intensity UVB is better than that of NB-UVB.

Key words: Ultraviolet; Guinea pigs; Keratinocytes; Hyperpigmentation; α-Melanocyte-stimulating hormone

中图分类号:

R758.4 1

张敏1,2,李春阳2, 王克玉2, 杨秀莉1. 不同中波紫外线对豚鼠皮肤色素沉着及α-黑素细胞刺激素表达的影响[J]. 山东大学学报(医学版), 2011, 49(3): 59-63.

ZHANG Min1,2， LI Chun-yang2， WANG Ke-yu2， YANG Xiu-li1. Effects of ultraviolet irradiation at different wavelengths on the melanin level and expression of α-melanocyte-stimulating hormone in the skin of guinea pigs[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(3): 59-63.

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