淋巴性脑水肿大鼠孤束背内侧亚核Glu、GABA及GAD67的时程变化

山东大学学报(医学版) ›› 2011, Vol. 49 ›› Issue (3): 64-.

淋巴性脑水肿大鼠孤束背内侧亚核Glu、GABA及GAD67的时程变化

宋希俊1，郑延红2，刘新民1，王立为1，赵晓民3，夏作理3

1.中国医学科学院北京协和医学院药用植物研究所药理毒理中心，北京100193；
2.兵器工业北京北方医院，北京 100089； 3.泰山医学院生命科学研究所，山东泰安 271000

收稿日期:2010-04-18 出版日期:2011-03-10 发布日期:2011-03-10
通讯作者: 刘新民（1962- ）,男，教授，博士生导师，主要从事神经药理研究。夏作理（1944- ）,男，教授，博士生导师，主要从事神经病学基础与临床研究。
作者简介:宋希俊（1969- ）,男，博士研究生，讲师，主要从事神经药理研究。 E-mail：gongxiangla@163.com

Temporal alterations of Glu, GABA and GAD67 in dmNTS in rats with lymphatic brain edema

SONG Xi-jun1, ZHENG Yan-hong2, LIU Xin-min1, WANG Li-wei1, ZHAO Xiao-min3, XIA Zuo-li3

1. Center for Pharmacology and Toxicology, Institute of Medicinal Plant Development, Chinese Academy of
Medical Sciences & Peking Union Medical College, Beijing 100193, China;
2. Beijing North Hospital, China North Industries Group Corporation, Beijing 100089, China;
3. Institute of Life Sciences, Taishan Medical College, Tai′an 271000, Shandong， China

Received:2010-04-18 Online:2011-03-10 Published:2011-03-10

摘要/Abstract

摘要：

目的观察淋巴性脑水肿（LBE）模型大鼠孤束背内侧亚核（dmNTS）内谷氨酸（Glu）、γ-氨基丁酸（GABA）的改变及谷氨酸脱羧酶亚型67（GAD67）表达的时程变化，并探讨其意义。方法应用免疫组化方法，观察LBE大鼠dmNTS内Glu、GABA及GAD67在颈淋巴引流阻滞后不同时间点的变化情况。结果 LBE组Glu改变及GAD67表达在颈淋巴引流阻滞后1d即开始升高，至第7天达高峰，后逐渐下降，于21d恢复；而GABA在颈淋巴引流阻滞后1d即开始降低，至第7天降至最低，后逐渐升高，于21d恢复。结论 LBE大鼠dmNTS内Glu和GABA的改变可能参与了血压调节功能的降低；GAD67早期表达逐渐增加，可能是机体的一种代偿性机制，有助于神经递质水平的平衡和恢复。

关键词: 淋巴性脑水肿；孤束背内侧亚核；谷氨酸；γ-氨基丁酸；谷氨酸脱羧酶亚型67；大鼠

Abstract:

Objective To investigate temporal alterations of glutamic acid (Glu), gamma-aminobutyric acid (GABA) and glutamic acid decarboxylase isoform 67 (GAD67) in the dorso-medial nucleus of tractus solitarii (dmNTS) in rats with lymphatic brain edema (LBE) induced by cervical lymphatic blockage (CLB). Methods Sprague-Dawley (SD) rats were randomly divided into three groups： the normal group, the sham operated group and the LBE group. Glu, GABA and GAD67 in dmNTS in rats were detected by immuohistochemistry 1, 3, 7, 11, 15 and 21 days after CLB or sham operation. Results Expressions of Glu and GAD67 increased 1 day after CLB, reached a peak on the 7th day, then gradually stepped down and recovered on the 21st day. Whereas reverse changes were observed in expression of GABA. No apparent difference was shown in the normal and sham operated groups. Conclusions Alterations of Glu and GABA may participate in the dysfunction of blood pressure regulation. Early ascending expression of GAD67 promotes transformation from Glu to GABA, which contributes to neuro-functional recovery in rats with LBE.

Key words: Lymphatic brain edema; Dorsomedial nucleus of tractus solitarii; Glutamic acid; Gamma-aminobutyric acid; Glutamic acid decarboxylase isoform 67; Rats

中图分类号:

R742

宋希俊1，郑延红2，刘新民1，王立为1，赵晓民3，夏作理3. 淋巴性脑水肿大鼠孤束背内侧亚核Glu、GABA及GAD67的时程变化[J]. 山东大学学报(医学版), 2011, 49(3): 64-.

SONG Xi-jun1, ZHENG Yan-hong2, LIU Xin-min1, WANG Li-wei1, ZHAO Xiao-min3, XIA Zuo-li3. Temporal alterations of Glu, GABA and GAD67 in dmNTS in rats with lymphatic brain edema[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2011, 49(3): 64-.

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