米非司酮对卵巢癌细胞系SKOV3增殖和CEACAM6表达的影响

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (5): 54-57.

米非司酮对卵巢癌细胞系SKOV3增殖和CEACAM6表达的影响

孙颖,郑晓静,李明江,赵兴波

山东大学附属省立医院妇产科, 济南 250021

收稿日期:2009-11-17 出版日期:2010-05-16 发布日期:2010-05-16
通讯作者: 赵兴波（1964- ）,男,博士,教授,主要从事妇科肿瘤方面的研究。 E-mail：zxb0626@126.com
作者简介:孙颖（1984- ）,女,硕士研究生,主要从事妇科肿瘤方面的研究。

Effect of mifepristone on proliferation and expression of CEACAM6 of human ovarian cancer cell line SKOV3

SUN Ying, ZHENG Xiaojing, LI Mingjiang, ZHAO Xingbo

Department of Obstetrics and Gynecology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China

Received:2009-11-17 Online:2010-05-16 Published:2010-05-16

摘要/Abstract

摘要：

目的观察米非司酮对卵巢癌细胞系SKOV3增殖和癌胚抗原相关黏附分子6（CEACAM6）表达的影响。方法不同浓度米非司酮（空白对照、1×10-7、1×10-6、1×10-5、5×10-5mol/L）干预卵巢癌细胞系SKOV3 24、48、72h；四甲基偶氮唑蓝(MTT)法检测米非司酮对SKOV3的抑制作用；逆转录聚合酶链反应（RTPCR）和蛋白印记（Western blot）检测不同浓度米非司酮干预下CEACAM6 mRNA和蛋白表达水平的差异,并进行半定量分析。结果与空白对照组比较,1×10-7和1×10-6mol/L米非司酮对SKOV3的生长没有明显的抑制作用（P＞0.05）；5×10-5mol/L米非司酮显著抑制SKOV3的生长（P＜0.01）,并降调SKOV3细胞中CEACAM6 mRNA和蛋白的表达水平（P＜0.05）。结论米非司酮可明显抑制SKOV3的体外增殖,其作用机制可能与抑制CEACAM6的表达有关。

关键词: 米非司酮；卵巢肿瘤；癌胚抗原相关黏附分子6

Abstract:

Objective To evaluate the effect of mifepristone on proliferation and expression of CEACAM6 of human ovarian cancer cell line SKOV3 in vitro. Methods Human ovarian cancer cell line SKOV3 was cultured with different concentrations (control group, 1×10-7, 1×10-6, 1×10-5, 5×10-5mol/L) of mifepristone(MIF) in vitro; MTT was used to examine the cell growth; and the semi-quantity of CEACAM6 mRNA and protein were detected by RT-PCR and western blot. Results Proliferation of SKOV3 cultured with 1×10-7, 1×10-6 mol/L of MIF was not significantly lower than the control group（P＞0.05）；5×10-5mol/L of MIF was able to significantly suppress the proliferation of SKOV3（P＜0.01）; Expressions of CEACAM6 mRNA and protein of SKOV3 in the control group were higher than that of 5×10-5mol/L of the mifepristone group（P＜0.05）. Conclusion Mifepristone could significantly suppress the proliferation of human ovarian cancer cell line SKOV3 in vitro. The action mechanisms may be associated with the suppression of expression of CEACAM6.

Key words: Mifipristone；Ovarian neoplasms；Carcinoembryonic antigen-related cell adhesion molecule 6

中图分类号:

R711.75

孙颖,郑晓静,李明江,赵兴波. 米非司酮对卵巢癌细胞系SKOV3增殖和CEACAM6表达的影响[J]. 山东大学学报(医学版), 2010, 48(5): 54-57.

SUN Ying, ZHENG Xiaojing, LI Mingjiang, ZHAO Xingbo. Effect of mifepristone on proliferation and expression of CEACAM6 of human ovarian cancer cell line SKOV3[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(5): 54-57.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed

[1]	鹿群，沈浣，王丛，郑兴邦，陈曦，梁蓉,魏丽惠. 脱氢表雄酮对卵巢反应不良患者体外受精-胚胎移植的影响[J]. 山东大学学报(医学版), 2013, 51(2): 84-.
[2]	乔真1，刘培淑2，韩冰1，王毓1. Smac小分子compound 3调节顺铂诱导卵巢癌细胞SKOV3凋亡的研究[J]. 山东大学学报(医学版), 2012, 50(6): 61-.
[3]	王月恒1，邢毅2，王玖玲1，代军3，李淑玲1. 多囊卵巢综合征患者血清内脂素及TNF-α的水平变化及其意义[J]. 山东大学学报(医学版), 2012, 50(2): 94-.
[4]	赵瑛，颜磊，张辉，于鹏，李明江，赵兴波. 精子相关抗原9在卵巢浆液性上皮肿瘤中的表达[J]. 山东大学学报(医学版), 2012, 50(2): 98-.
[5]	丁萍萍，李强. 溶血磷脂酸促卵巢癌细胞增殖机制的研究[J]. 山东大学学报(医学版), 2011, 49(7): 35-.