地前素M抗前列腺肿瘤活性的初步探讨

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (5): 18-22.

地前素M抗前列腺肿瘤活性的初步探讨

许爱辉1，潘喆2，蒋汉明1，孔峰1，胡志敏3，苑辉卿1

山东大学 1.医学院生物化学与分子生物学研究所，济南 250012；
2．第二医院内分泌科，济南 250033； 3.齐鲁医院检验科，济南 250012

收稿日期:2009-03-18 出版日期:2010-05-16 发布日期:2010-05-16
通讯作者: 苑辉卿（1965- ），女，教授，博士生导师，主要从事肿瘤分子生物学研究。 E-mail:lyuanhq@sdu.edu.cn
作者简介:许爱辉（1982- ），女，硕士研究生；潘喆（1982- ），女，医师，主要从事前列腺癌分子生物学研究。
基金资助:
国家自然科学基金资助项目（30772594）

Marchantin M displays growth Inhibitory effects on human prostse cancer cells

XU Aihui1, PAN Zhe2, JIANG Hanming1， KONG Feng1， HU Zhimin3， YUAN Huiqing1

1. Department of Biochemistry and Molecular Biology, School of Medicine, Shandong University, Jinan 250012, China;
2. Department of Endocrinology, The Second Hospital of Shandong University, Jinan 250033, China;
3. Department of Clinical Laboratory, QiLu Hospital of Shandong University, Jinan 250012, China

Received:2009-03-18 Online:2010-05-16 Published:2010-05-16

摘要/Abstract

摘要：

目的检测苔藓植物联苄类化合物地前素M的抗肿瘤活性，并初探其对前列腺癌细胞的作用机制。方法 MTT法检测地前素M对人白血病细胞株（K562）、人肝癌细胞株（HepG2）、人乳腺癌细胞株（MCF-7）、人前列腺癌细胞株（LNCaP、DU145及PC3）和人正常视网膜色素上皮细胞株（hTERT-RPE1）的增殖影响；溴脱氧尿嘧啶核苷（BrdU）掺入法检测地前素M对PC-3细胞增殖的作用，流式细胞术检测细胞周期和凋亡的变化；Western blotting检测细胞周期相关蛋白的表达；4′,6二脒基-2苯基吲哚 (DAPI)染色法检测PC-3细胞核的变化。结果地前素M可抑制K562、HepG2、MCF-7、LNCaP、DU145、PC3细胞的增殖，其对PC-3细胞增殖的抑制作用尤为显著，而对hTERT-RPE1细胞增殖的抑制作用较弱。BrdU结果显示,地前素M能够降低BrdU在PC3细胞中的掺入；流式细胞术示，地前素M将PC3细胞阻滞在G0-G1期，且出现明显的subG1峰，并伴随周期相关蛋白的变化；DAPI示，细胞核发生凋亡的特征性变化。结论地前素M具有抑制肿瘤细胞增殖的活性，并引起PC-3细胞周期阻滞，诱导细胞凋亡。

关键词: 地前素M；细胞凋亡；细胞周期；前列腺癌细胞

Abstract:

Objective To Investigate the antiproliferational effect of Marchantin M on human carcinoma cells. MethodsThe cytotoxic effect of Marchantin M was assessed by MTT assay. It′s inhibitory effect on the growth of PC-3 cells was measured by incorporation of BrdU. The cell cycle progression and apoptosis of PC-3 cells were determined by the flow cytometry assay. Alterations in the expression of cell cycle related proteins in PC-3 cells exposed to Marchantin M were determined by Western blotting. Nucleic morphological changes of Marchantin M-treated PC-3 cells were determined by staining cells with DAPI. Results Marchantin M led to a viability inhibition of K562, HepG2, MCF-7, LNCaP, DU145 and PC-3 cells in a dose-dependent manner. However, Marchantin M had no significant inhibitory effects on the growth of normal human retina pigment epithelial cells（hTERT-RPE1）. Flow cytometry assay indicated that treatment with Marchantin M resulted in cell cycle arrest at G0/G1 phase. Further examination showed that the expression of cell cycle related proteins were changed after treatment. DAPI staining assay showed that Marchantin M treatment caused the increase in fraction of apoptotic cells. Conclusion These observations indicate that Marchantin M is a promising compound that shows antitumor activity against protate cancer cells.

Key words: Marchantin M; Apoptosis; Cell cycle; Prostate carcinoma cells

中图分类号:

737.25

许爱辉1，潘喆2，蒋汉明1，孔峰1，胡志敏3，苑辉卿1. 地前素M抗前列腺肿瘤活性的初步探讨[J]. 山东大学学报(医学版), 2010, 48(5): 18-22.

XU Aihui1, PAN Zhe2, JIANG Hanming1， KONG Feng1， HU Zhimin3， YUAN Huiqing1. Marchantin M displays growth Inhibitory effects on human prostse cancer cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(5): 18-22.

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