大蒜素与两性霉素B体外联合应用抑制白色念珠菌菌丝形成的研究

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (5): 23-27.

大蒜素与两性霉素B体外联合应用抑制白色念珠菌菌丝形成的研究

王晓燕,齐眉,李玉瑭,孙允东,张晓丽,刘娟,程轶喆,于晗,周亚滨

山东大学医学院病原生物学研究所山东省感染与免疫重点实验室, 济南 250012

收稿日期:2009-12-15 出版日期:2010-05-16 发布日期:2010-05-16
通讯作者: 周亚滨（1965- ）女,副教授,主要从事病原微生物致病及治疗的研究。 E-mail: zyb@sdu.edu.cn
作者简介:王晓燕（1981- ）,女,硕士研究生,主要从事病原微生物致病及治疗的研究。 E-mail:wang61xiao121yan@yahoo.cn
基金资助:
山东省自然科学基金资助项目（2009ZRB02561）

Study of combination of allicin and amphotericin B inhibitinghyphae formation of Candida albicans in vitro

WANG Xiaoyan, QI Mei, LI Yutang, SUN Yundong, ZHANG Xiaoli, LIU Juan, CHENG Yizhe, YU Han, ZHOU Yabin

Department of Pathogenic Biology, Shandong Univeristy School of Medicine
Provincial Key Laboratory of Infection and Immunology, Jinan 250012, China

Received:2009-12-15 Online:2010-05-16 Published:2010-05-16

摘要/Abstract

摘要：

目的研究大蒜素与两性霉素B体外联合应用对白色念珠菌菌丝形成的抑制作用,并检测联合应用时细胞毒性的变化。方法采用微量稀释法测定两类药物联合抗白色念珠菌的作用,采用FICI法对联用结果进行评价。用10％小牛血清RPMI 1640培养基诱导菌丝形成,检测大蒜素与两性霉素B联合应用对白色念珠菌芽管率和菌丝形成的影响。通过对细胞形态的观察及MTT法检测联合用药后对人宫颈上皮细胞H8毒性的变化。结果两种药物联用对白色念珠菌表现为协同作用,大蒜素能够加强两性霉素B抑制菌丝形成的能力,联合用药后能够显著降低对H8细胞的毒性。结论大蒜素能够提高两性霉素B抗白色念珠菌的作用,是临床上减少两性霉素B用量,减轻其副作用的最佳选择。

关键词: 大蒜；两性霉素B；药物相互作用；念珠菌,白色；菌丝

Abstract:

Objective To explore combination of allicin and amphotericin B inhibiting effect of filament formation against Candida albicans in vitro, and detect cytotoxicity variation under combination of two drugs. Methods The effect of combination of two drugs against Candida albicans was determined by the checkerboard microdilution method, and the fractional inhibitory concentration index(FICI)was calculated. The filament formation was induced in 10% calf serum-1640.The impact of combination of allicin and amphotericin B on the rate of hyphae and filament formation of Candida albicans was determined.The H8 cytotoxicity variation under combination of two drugs was detected through cellular appearance and MTT. Results Drugs combined together acted synergistically against Candida albicans, and allicin could enhance the ability of amphotericin B to inhibit filament formation.The effect of cytotoxicity was reduced remarkably under combination of two drugs. Conclusion Allicin largely enhances the anti-Candida albicans activity of amphotericin B, and it is a best selection in clinical applications for reducing dose and adverse effect of amphotericin B.

Key words: Garlic; Amphotericin B; Drug interactions; Candida albicans; Hyphae

中图分类号:

R978.5

王晓燕,齐眉,李玉瑭,孙允东,张晓丽,刘娟,程轶喆,于晗,周亚滨. 大蒜素与两性霉素B体外联合应用抑制白色念珠菌菌丝形成的研究[J]. 山东大学学报(医学版), 2010, 48(5): 23-27.

WANG Xiaoyan, QI Mei, LI Yutang, SUN Yundong, ZHANG Xiaoli, LIU Juan, CHENG Yizhe, YU Han, ZHOU Yabin. Study of combination of allicin and amphotericin B inhibitinghyphae formation of Candida albicans in vitro[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(5): 23-27.

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