药物干预对哮喘小鼠模型Th1/Th2细胞因子表达的影响

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (10): 48-50.

药物干预对哮喘小鼠模型Th1/Th2细胞因子表达的影响

王岷1,2，陈文靖1，杨琳红1，刘磊1, 吴大玮2，刘俊丽1

1. 山东中医药大学第二附属医院呼吸科，济南 250001；2. 山东大学齐鲁医院ICU科，济南 250012

收稿日期:2010-04-23 出版日期:2010-10-16 发布日期:2010-10-16
通讯作者: 吴大玮（1955- ），男，教授，主要从事支气管哮喘COPD及急危重症等的研究。 E-mail:wdw.55@163.com
作者简介:王岷(1969- )，女，主治医师,主要从事支气管哮喘、慢性阻塞性肺疾病等的研究。 E-mail:sywangm@yahoo.com.cn

Interfering effects of thymosine and dexamethasone on TH1/TH2cytokines in the asthma mouse model

WANG Min1,2, CHEN Wen-jing1, YANG Lin-hong1, LIU Lei1, WU Da-wei2, LIU Jun-li1

1. Department of Respirology, Second Affiliated Hospital of Shandong University of
Traditional Chinese Medicine, Jinan 250001， China；
2. Department of ICU, Qilu Hospital of Shandong University, Jinan 250012， China

Received:2010-04-23 Online:2010-10-16 Published:2010-10-16

摘要/Abstract

摘要：

目的探讨胸腺素-α和地塞米松对辅助型T淋巴细胞1型/辅助型T淋巴细胞2型（Th1/Th2）细胞因子在哮喘发病过程中的干预作用及其临床应用价值。方法将小鼠分为对照组、哮喘模型组、胸腺素干预组、地塞米松干预组。采用酶联免疫吸附试验（ELISA）法，测定支气管肺泡灌洗液（BALF）中白细胞介素-4（IL-4）、白细胞介素-5（IL-5）和干扰素γ（IFNγ）的水平。结果与对照组相比，哮喘组的嗜酸性粒细胞（EOS）、淋巴细胞、IL4、IL5升高, IFNγ下降（P<0.05）。与哮喘组相比，胸腺素组和地塞米松组的EOS、IL-4、IL-5减少，胸腺素组IFN-γ增加（P<0.05）。对照组与哮喘组、哮喘组与胸腺素组、哮喘组与地塞米松组之间的IL4/IFNγ相比，差异均有统计学意义（P均<0.05）。结论 Th2优势反应在哮喘的发病机制中起重要作用，胸腺素-α可明显提高IFN-γ、Th1的水平，地塞米松可以降低IL-4、IL-5及Th2水平。

关键词: T淋巴细胞,辅助诱导；胸腺素；地塞米松；小鼠

Abstract:

Objective To explore the role of Th1/Th2 cytokines in the pathogenic process of asthma,interfering effects of thymosine-α and dexamethasone-on Th1/Th2 and their clinical values. Methods An asthma model was set up and thymosine-α and dexamethasone were given. The rats were divided into the normal control group,the asthmic group,the thymosin group and the dexamethasone group.Levels of IL-4, IL-5 and IFN-γin bronchoalveolar lavage fluid(BALF) were detected by ELISA. Results There was a significant increase in the numbers of EOS and lymphocytes and levels of IL-4 and IL-5, while there was a significant decrease in the level of IFN-γ in the asthmic group compared with the normal control group (P<0.05). Number of EOS and levels of IL-4 and IL-5 decreased in the thymosine dexamethasone groups compared with the asthmic group, and the difference was significant (P<0.05). The level of IFN-γ in the thymosin group increased compared with the asthmic group and the difference was significant(P<0.05). For IL-4/IFN-γ, there were notable differences between the normal group and the asthmic group,between the asthmic group and the thymosin group,and between the asthmic group and the dexamethasone group(P<0.05). Conclusion The Th2 dominant reaction play an important role in the pathogenesis of asthma.Thymosin-α can notably increase the level of IFN-γ. Dexamethasone can lower the levels of IL-4 and IL-5.

Key words: T-lymphocyte, helper-inducer; Thymosin; Dexamethasone; Mice

中图分类号:

R562.2+5

王岷1,2，陈文靖1，杨琳红1，刘磊1, 吴大玮2，刘俊丽1. 药物干预对哮喘小鼠模型Th1/Th2细胞因子表达的影响[J]. 山东大学学报(医学版), 2010, 48(10): 48-50.

WANG Min1,2, CHEN Wen-jing1, YANG Lin-hong1, LIU Lei1, WU Da-wei2, LIU Jun-li1. Interfering effects of thymosine and dexamethasone on TH1/TH2cytokines in the asthma mouse model[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(10): 48-50.

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