三七总皂苷对oxLDL诱导的人脐静脉内皮细胞CD40, VCAM-1表达的影响

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (10): 14-19.

三七总皂苷对oxLDL诱导的人脐静脉内皮细胞CD40, VCAM-1表达的影响

刘桂林1，窦迎春2，刘粉叶3，张晴4，任敏5，张继东5

1.山东中医药大学第二附属医院心内科，济南 250001； 2.山东中医药大学基础医学院，济南 250355；
3.山东大学附属省立医院中医科, 济南 250021； 4.山东省医学科学院附属医院，济南 250031；
5.山东大学齐鲁医院中医科, 济南 250012

收稿日期:2010-06-28 出版日期:2010-10-16 发布日期:2010-10-16
通讯作者: 张继东，男，教授。主要从事中西医结合防治心血管疾病研究。E-mail: drzhang0103@163.com
作者简介:刘桂林（1972- ）, 女，医学博士，主治医师，研究方向为中西医结合防治心血管疾病研究。

Effect of total panax notoginsenosides on expression of CD40, VCAM-1 induced by oxLDL in human umbilical vein endothelial cells

LIU Gui-lin1, DOU Ying-chun2, LIU Fen-ye3, ZHANG Qing4, REN Min5, ZHANG Ji-dong5

1. Department of Cardiology, Second Hospital Affiliated to Shandong University of
Traditional Chinese Medicine, Jinan 250001, China;
2. College of Basic Medical Sciences, Shandong University of Traditional Chinese Medicine, Jinan 250355, China;
3. Department of Traditional Chinese Medicine, Shandong Provincial Hospital Affiliated to
Shandong University, Jinan 250021, China;
4. Hospital Affiliated to Shandong Academy of Medical Science, Jinan 250031, China;
5. Department of Traditional Chinese Medicine, Qilu Hospital of Shandong University, Jinan 250012, China

Received:2010-06-28 Online:2010-10-16 Published:2010-10-16

摘要/Abstract

摘要：

目的探讨三七总皂苷(TPNS)对氧化型低密度脂蛋白（oxLDL）诱导的人脐静脉内皮细胞（HUVECs）白细胞分化抗原40（CD40）、血管细胞粘附分子-1（VCAM-1）表达的影响。方法原代培养HUVECs。细胞分为空白组、刺激组、小剂量（100mg/L）TPNS组、大剂量（200mg/L）TPNS组及辛伐他汀组并予相应处理。采用MTT法测定HUVECs活性，实时定量RT-PCR检测VCAM1的基因表达，Western blot 分析CD40蛋白表达量。结果 TPNS及辛伐他汀均可以升高HUVECs的活性（P＜0.05, P＜0.001），下调HUVECs表达免疫炎症因子CD40 (P＜0.05, P＜0.01)及VCAM-1（P均＜0.001）的水平。且大剂量TPNS作用优于小剂量TPNS（P＜0.05）。结论 TPNS能够减轻oxLDL对内皮细胞的损伤，降低内皮细胞免疫炎症因子的表达，在防治动脉粥样硬化中具有重要作用。

关键词: 三七总皂苷；氧化型低密度脂蛋白；人脐静脉内皮细胞；白细胞分化抗原40；血管细胞粘附分子-1

Abstract:

Objective To explore the effect of total panax notoginsenosides (TPNS) on expression of cell differentiation antigen 40 (CD40) and vascular cell adhesion molecule-1 (VCAM-1) in human umbilical vein endothelial cells (HUVECs) induced by oxidized low density lipoprotein (oxLDL). Methods Primary culture HUVECs cells were divided into five groups: the control, the model, the low dose (100mg/L) the TPNS, high dose (200mg/L) TPNS and the Simvastain group. Apart from the control group, cells in other groups were given corresponding treatment. Cytoactivity was measured by MTT. mRNA expression of VCAM-1 was detected by realtime quantitative RT-PCR. Protein expression of CD40 was measured by Western blot. Results TPNS and Simvastatin could up-regulate the cytoactivity of HUVECs(P＜0.05, P＜0.001), and down-regulate expression of CD40(P＜0.05, P＜0.01), VCAM-1（P＜0.001）in HUVECs induced by oxLDL. In addition, the effect of high dose TPNS was superior to the low dose TPNS (P＜0.05). Conclusion TPNS can relieve injury and lower expression of immune inflammatory factors in HUVECs induced by oxLDL. TPNS has a potential effect in preventing and treating atherosclerosis.

Key words: Total panax notoginsenosides; Oxidized low density lipoprotein; Human umbilical vein endothelial cells; Cell differentiation antigen 40; Vascular cell adhesion molecule-1

中图分类号:

R285.5

刘桂林1，窦迎春2，刘粉叶3，张晴4，任敏5，张继东5. 三七总皂苷对oxLDL诱导的人脐静脉内皮细胞CD40, VCAM-1表达的影响[J]. 山东大学学报(医学版), 2010, 48(10): 14-19.

LIU Gui-lin1, DOU Ying-chun2, LIU Fen-ye3, ZHANG Qing4, REN Min5, ZHANG Ji-dong5. Effect of total panax notoginsenosides on expression of CD40, VCAM-1 induced by oxLDL in human umbilical vein endothelial cells[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(10): 14-19.

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