sFRP-1、Wnt-1在宫颈癌及癌前病变中的表达及意义

山东大学学报(医学版) ›› 2010, Vol. 48 ›› Issue (1): 97-101.

sFRP-1、Wnt-1在宫颈癌及癌前病变中的表达及意义

苏姗1，阚艳艳1，田永杰2

山东大学 1. 医学院，济南 250012; 2. 附属省立医院妇产科，济南 250021

收稿日期:2009-08-06 出版日期:2010-01-16 发布日期:2010-01-16
通讯作者: 田永杰（1964- ），主任医师，教授，主要从事妇科肿瘤和普通妇科的研究。
作者简介:苏姗（1982- ），女，硕士研究生,主要从事宫颈癌及癌前病变的研究

Expressions and significances of sFRP-1 and Wnt-1 in cervical carcinoma and precancerous lesions

SU Shan1, KAN Yanyan1， TIAN Yongjie2

1. School of Medicine, Shandong University, Jinan 250012, China;
2. Department of Obstetrics and Gynecology, Provincial Hospital Affiliated to Shandong University, Jinan 250021, China

Received:2009-08-06 Online:2010-01-16 Published:2010-01-16

摘要/Abstract

摘要：

目的探讨分泌性卷曲相关蛋白1（sFRP-1）和Wnt-1蛋白在宫颈上皮内瘤变（CIN）和宫颈癌中的表达及意义。方法采用免疫组化SABC法测定sFRP-1、Wnt-1蛋白在正常宫颈组织、低度上皮内瘤变组织（LSIL）、高度上皮内瘤变组织（HSIL）和宫颈癌中的表达水平及分布；并用反转录聚合酶链反应(RT-PCR)检测sFRP-1、Wnt1mRNA的表达水平。结果 sFRP-1在宫颈癌中的表达明显低于HSIL及LSIL，同时LSIL低于正常宫颈组织。Wnt-1在宫颈癌中的表达明显高于HSIL及LSIL，同时HSIL高于LSIL，LSIL高于正常宫颈组织。结论 Wnt信号通路抑制因子sFRP-1的过低表达及其主要成员Wnt-1的过度表达在由CIN发展到宫颈癌的过程中起了促进作用。

Abstract:

Objective To investigate expressions of secreted frizzled related protein-1 (sFRP-1) and Wnt-1 in patients with cervical intraepithelial neoplasia and cervical carcinoma. Methods sFRP-1 and Wnt-1 were detected by immunohistochemistry（SABC）in normal cervical tissue, low-grade squamous intraepithelial lesions（LSIL), high-grade squamous intraepitheliallesions (HSIL) and cervical carcinoma， and expressions of sFRP-1 and Wnt-1mRNAin normal cervical tissue and cervical carcinoma were detected using reverse transcription polymerase chain reaction(RT-PCR). Results Expression of sFRP-1 in cervical carcinoma was significantly weaker than in HSIL and LSIL, and in LSIL was weaker than in normal cervical tissues. Expression of Wnt-1 was strongest in cervical carcinoma and lowest in normal cervical tissues, and it was stronger in HSIL than in LSIL. Conclusion Lower expression of sFRP-1 and over expression of Wnt-1 play important roles in the development from cervical intraepithelial neoplasia to cervical carcinoma.

Key words: Secreted frizzled related protein-1； Wnt-1 protein； Cervical intraepithelial neoplasia； Cervical neoplasia

中图分类号:

R711.74

苏姗1，阚艳艳1，田永杰2. sFRP-1、Wnt-1在宫颈癌及癌前病变中的表达及意义[J]. 山东大学学报(医学版), 2010, 48(1): 97-101.

SU Shan1, KAN Yanyan1， TIAN Yongjie2. Expressions and significances of sFRP-1 and Wnt-1 in cervical carcinoma and precancerous lesions[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2010, 48(1): 97-101.

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