联合用药减少铜绿假单胞菌
耐药突变体的体外研究

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (9): 25-27.

联合用药减少铜绿假单胞菌
耐药突变体的体外研究

刘明涛^1,孙恩华²,毕少杰¹,于瑞丛²,李玉¹

山东大学齐鲁医院 1. 呼吸内科； 2. 细菌室, 济南 250012

收稿日期:2009-03-01 发布日期:2009-09-16
通讯作者: 李玉(1963- )，男，主任医师，硕导，主要从事肺部感染性疾病的研究。Email：qlliyu@tom.com
作者简介:刘明涛(1984- )，男，硕士研究生，主要从事细菌耐药的研究。Email：842764917@qq.com
基金资助:
山东省科技攻关资助项目（2006GG2202013）。

Antibiotics in reducing resistant mutants of Pseudomonas 
aeruginosa in vitro

LIU Mingtao¹， SUN Enhua²，BI Shaojie¹，　YU Ruicong²， LI Yu¹

1. Department of Pulmonary Disease； 2. Department of Bacteria Room, Qilu Hospital of Shandong University, Jinan 250012, China

Received:2009-03-01 Published:2009-09-16

摘要/Abstract

摘要：

目的在体外探讨环丙沙星(CIP)、阿米卡星(AMK)、头孢他啶(CAZ)两两联合是否有利于减少铜绿假单胞菌耐药突变体的产生。方法分别应用琼脂平板稀释法和棋盘格法测定单独以及联合之后的最低抑菌浓度（MIC），采用琼脂平板稀释法测定单独及联合时对铜绿假单胞菌的防耐药突变浓度（MPC），并计算选择指数（SI）即MPC/MIC，根据SI下降的程度来判断防耐药突变能力。结果CIP、AMK、CAZ单药对铜绿假单胞菌的SI分别为16、16、＞32。两两联合后各自单药的SI分别为:CIP+AMK (CIP8，AMK4); CIP+CAZ (CIP4，CAZ8)；AMK+CAZ(AMK8，CAZ4)。结论以上3种药物任意两两联合均可以降低单独用药时对铜绿假单胞菌的SI，有利于防止铜绿假单胞菌耐药突变体的产生。

关键词: 防耐药突变浓度, 药物疗法，联合, 假单胞菌，铜绿

Abstract:

To study whether the combinations of two drugs of ciprofloxacinamikacin(CIP), ciprofloxacinceftazidime(AMK) and ceftazidimeamikacin(CAZ) will decrease drugresistant mutants of Pseudomonas aeruginosa in vitro. MethodsMinimum inhibitory concentration (MIC) of CIP,AMK, and CAZ alone was determined by an agar plates dilution method and MIC of the combinations of them was determined by a checkerboard method. Mutant prevention concentration (MPC) of each single and of combinations were determined by the agar plates dilution method, and then the value of SI(MPC/MIC) was obtained. Capacity in preventing the appearance of drugresistant mutants was determined based on degree that SI dropped. ResultsSI of CIP, AMk, and CAZ was 16, 16, and ＞32, and of ciprofloxacinamikacin(CIP8; AMK4); ciprofloxacinceftazidime(CIP4; CAZ8); ceftazidimeamikacin(AMK8; CAZ4). ConclusionCompared with each one alone, two drug combinations can decrease MPC and SI to pseudomonas aeruginosa and restrain the drugresistant mutants.

Key words: Drug therapy, combination； Pseudomonas aeruginosa; M utant prevention concentration

中图分类号:

R978.1

. 联合用药减少铜绿假单胞菌
耐药突变体的体外研究[J]. 山东大学学报(医学版), 2009, 47(9): 25-27.

参考文献

多维度评价

Viewed

Full text

Abstract

Cited

Shared

Discussed