碘化钾对人体皮肤成纤维细胞增殖活性的影响

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (8): 55-57.

碘化钾对人体皮肤成纤维细胞增殖活性的影响

孙本成¹，张芮²，王一兵¹，冯永强¹，杨高松¹

1. 山东大学附属省立医院烧伤整形外科，济南 250021； 2. 山东省医学高等专科学校病理教研室，济南 250002

收稿日期:2009-04-09 发布日期:2009-08-16
通讯作者: 王一兵(1963- ),男,主任医师, 主要从事烧伤整形外科。Email: wyb0616@163.com
作者简介:孙本成(1979- ),男,医师，硕士研究生,主要从事烧伤整形外科研究。
基金资助:
山东省国际科技合作项目［鲁科外字（2005）114号］

Effect of KI on proliferative activity of human skin fibroblasts

SUN Bencheng¹, ZHANG Rui², WANG Yibing¹, FENG Yongqiang¹, YANG Gaosong^1

1. Department of Burn and Plastic Surgery, Provincial Hospital Affiliated to Shandong University， Jinan 250021, China;
2. Department of Pathology, Shandong Medical College, Jinan 250002, China

Received:2009-04-09 Published:2009-08-16

摘要/Abstract

摘要：

目的探讨不同浓度的碘化钾（KI）对人体皮肤成纤维细胞(HSF)增殖活性的影响。方法将体外培养的ＨＳＦ分成7组含不同浓度的KI（0、1?000、3?000、5?000、7?000、9?000、11?000?μg/L），每组含8个平行样本。完全培养基分别培养24?h后，观察HSF形态变化，用流式细胞术和酶联免疫吸附试验（ELISA）方法检测HSF增殖活性。结果KI的浓度在1?000、3?000、5?000?μg/L时成纤维的增殖活性随着碘浓度的增加而升高（P＜0.05）；5?000、7?000?μg/L组细胞增殖活性最高，两组间增殖活性差异无统计学意义（P＞0.05）；7?000、9?000、11?000?μg/L?时HSF增殖活性随着KI浓度的增加而降低（P＜0.05）。结论KI浓度在5?000?μg/L以下会促进HSF增殖活性，高于7?000?μg/L时反而会抑制HSF增殖活性。

关键词: 成纤维细胞；碘化钾；细胞培养；增殖

Abstract:

To study the effects of KI on proliferative activity of human skin fibroblasts at different doses. MethodsHuman skin fibroblasts (HSF) were treated with KI 0, 1?000,3?000,5?000,7?000,9?000, and 11?000?μg/L for 24?h and to observe the morphology of fibroblasts. The methods of FCM and ELISA were used to detect the proliferation activity of human skin fibroblasts. ResultsKI at a certain range of concentration(＜5?000?μg/L)could significantly increase proliferative activity of human skin fibroblasts but decreases the proliferative activity at a certain range of concentration(＞7?000?μg/L). The proliferative activity was the highest from 5?000?μg/L to 7?000?μg/L, but there were no significant differences between them. ConclusionKI with an exposure level＜5?000?μg/L can increase the proliferative activity of human skin fibroblasts, whereas it decrease the activity when its exposure ＞7?000?μg/L.

Key words: Fibroblasts; Potassium iodide；Cell culture; ProliferationThorn

中图分类号:

R602

. 碘化钾对人体皮肤成纤维细胞增殖活性的影响[J]. 山东大学学报(医学版), 2009, 47(8): 55-57.

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