靶向survivin基因的RNAi诱导喉癌Hep2细胞凋亡的研究

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (8): 58-61.

靶向survivin基因的RNAi诱导喉癌Hep2细胞凋亡的研究

李红，谢睿，杨桦，镡旭民

第三军医大学附属新桥医院耳鼻咽喉头颈外科，重庆 400037

收稿日期:2009-03-17 发布日期:2009-08-16
作者简介:李红（1968- ），男，博士，副主任医师，主要从事耳鼻咽喉-头颈部肿瘤的基础研究和临床治疗。Email:leehong7118@yahoo.com.cn
基金资助:
国家自然科学基金资助（No.30672299）

RNAi of targeted against survivin induced apoptosis of laryngeal carcinoma cell line hep2

LI Hong, XIE Rui, YANG Hua, CHAN Xumin

Department Otorhinolaryngology and HeadNeck Surgery, Xinqiao Hospital Affiliated to Third Military Medical University, Chongqing 400037, China

Received:2009-03-17 Published:2009-08-16

摘要/Abstract

摘要：

目的通过构建RNAi表达载体，转染喉癌细胞株Hep2，观察其对survivin表达的抑制作用。方法构建针对survivin基因的干扰载体，用脂质体转染喉癌细胞Hep2，分析干扰载体对survivin表达的抑制情况，检测细胞凋亡。结果survivin在喉癌细胞Hep2中呈高表达，其基因序列与GenBank序列对比一致，干扰载体pGensil/survivin能抑制survivin的表达，抑制率为68%，并诱导了喉癌细胞的凋亡。结论针对survivin的小分子RNA可以有效抑制喉癌细胞Hep2中survivin蛋白的表达，并促进喉癌细胞凋亡。

关键词: RNAi喉肿瘤；　Survivin基因；细胞凋亡

Abstract:

To study the inhibitory effects of RNAi expression vector suppressing expression of survivin and inducing the apoptosis of laryngeal carcinoma cell line Hep2. MethodsRNAi expression vectors targeting survivin were successfully constructed, and then were transfected into Hep2 cells with liposomes. Expression of survivin mRNA was detected and the rate of cell apoptosis was measured. ResultsThere was a high expression of survivin in Hep2 cells. The DNA sequence was in accordance with the survivin gene in the GENEBANK. The survivin expression was inhibited 68.5% by pGenesil/survivin RNAi expression vectors, and the apoptosis of cells increased. ConclusionThe RNAi expression vector can effectively inhibit survivin expression and induce the apoptosis in Hep2 cells.

Key words: RNAi; Laryngeal squamous cell cancer; Survivin gene; Apoptosis

中图分类号:

R739.65

. 靶向survivin基因的RNAi诱导喉癌Hep2细胞凋亡的研究[J]. 山东大学学报(医学版), 2009, 47(8): 58-61.

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