关键词: 黑色素瘤, 瘤内化疗, 无细胞短棒状杆菌制剂, 联合治疗, 小鼠，近交C57BL

Abstract:

Objective  To explore the effects of non-cell corynebacterium parvum product（NCPP） combined with slow intra-tumor release of drugs on B16 melanoma in mice．  Methods  Tumor-bearing mice were randomly divided intofour groups： the normal saline group， the slow intra-tumor release drug group, the NCPP group and the drug combination group． Seven days later， mice were treated again and the gross tumor volume was determined． Then 2 weeks later， all the tumor-bearing mice were sacrificed to measure tumor weight. Pathologic changes of tumors were observed under light microscopy. Infiltrations of T cells were determined in the splenic organs．  Results   Drug combination can effectively contain the tumor growth (P＜0．05). HE staining results showed that there was a large area necrotic tissue in the tumor(P＜0．05)，　and CD8+ T cells were significantly increased in the drug combination group(P＜0．01)．  Conclusion NCPP combined with slow intra-tumor release drugs is one of the effective management method for directly inhibiting tumor growth．

Key words: Melanoma； Intratumoral chemotherapy； Non-cell corynebacterium parvum product； Drug combination； Mice， inbred C57BL