蜕膜组织杀伤细胞免疫球蛋白样受体与不明原因早期复发性流产的相关性

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (12): 81-85.

蜕膜组织杀伤细胞免疫球蛋白样受体与不明原因早期复发性流产的相关性

徐金娥^1,2，王珊¹，赵跃然¹，陈子江¹

1. 山东大学附属省立医院生殖医学中心，济南 250021； 2. 青岛大学医学院附属医院生殖医学科，山东青岛 266003

收稿日期:2009-05-20 出版日期:2009-12-16 发布日期:2009-12-16
通讯作者: 陈子江（1959- ），女，教授，博导，主要从事生殖医学的研究。Email: zjchen@yahoo.com
作者简介:徐金娥（1971- ），女，博士研究生，主要从事生殖医学的研究。
基金资助:
国家重点基础研究发展计划（973项目）（2005CB5227054）。

Association of killerimmunoglobulinlike receptors in the decidua and unexplained early recurrent spontaneous abortion

XU Jine ^1,2 , WANG Shan ¹, ZHAO Yaoran ¹ , CHEN Zijiang ¹

1. Center for Reproductive Medicine, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, China;
2. Department for Reproductive Medicine, Affiliated Hospital to Medical College of Qingdao University, Qingdao 266003, China

Received:2009-05-20 Online:2009-12-16 Published:2009-12-16

摘要/Abstract

摘要：

目的探讨蜕膜组织杀伤细胞免疫球蛋白样受体（KIR）与不明原因早期复发性流产（URSA）易感性的关系。方法留取URSA妇女（病例组，36例）及正常早孕妇女（对照组，34例）的蜕膜组织，采用酚-氯仿法抽提基因组DNA，序列特异性引物聚合酶链反应检测蜕膜组织14种KIR基因的表达；Trizol一步法提取总RNA，逆转录聚合酶链反应分析KIR mRNA的表达率，荧光实时定量聚合酶链反应分析KIR2DL1、2DL3 mRNA的相对表达量。结果① 14种KIR基因均以不同频率表达；KIR2DS1基因的表达率在病例组与对照组中分别为60.27%、41.18%，两组比较差异有统计学意义（P＜0.05)；② 6种抑制性KIR mRNA在蜕膜组织中均以不同频率表达，两组的表达率无统计学差异；抑制性KIR mRNA的表达率与KIR基因的表达率并不完全一致；③ KIR2DL1 mRNA及KIR2DL3 mRNA在病例组与对照组中的相对表达量分别为699.37±259.78、1?002.96±476.98和625.93±234.81，1?216.12±425.96，两组比较差异无统计学意义（P均＞0.05)。结论蜕膜组织活化性KIR2DS1基因的表达率增加，抑制性、活化性KIR基因失衡，可能与URSA的发生有关。

关键词: 杀伤细胞免疫球蛋白样受体；复发性流产；蜕膜；基因

Abstract:

To study the relationship between killerimmunoglobulinlike receptors in the decidua and early unexplained recurrent spontaneous abortion(URSA). MethodsDecidual samples were obtained from 36 patients suffering from unexplained recurrent spontaneous abortion and 34 normal pregnant women. Genespecific PCR amplification (PCRSSP) was used to determine the individuam KIR genotypes in women experiencing URSA and the controls. Reverse transcription polymerase chain reaction(RTPCR) was used to detect the individual KIR mRNA. KIR2DL1 mRNA and KIR2DL3 mRNA levels in the decidua from URSA and normal pregnancies were quantified by real time RTPCR. Results① Prevalence of activating KIR genes was higher in URSA patients than in the controls. Among women experiencing URSA, the expressive rate of KIR2DS1 was 60.27% in patients and 41.18% in the controls(P＜0.05). ② Transcripts of inhibitory KIRs mRNA were detected by RTPCR in individuals. The frequencies of inhibitory KIRs mRNA did not differ between women with URSA and the normal controls. No activating KIRs mRNA was detected. ③ The relative level of KIR2DL1 mRNA did not show significant differences between the two groups (699.37±259.78 vs　1?002.96±476.98, P＜0.05), but the KIR2DL3 mRNA did (625.93±234.81 vs 1216.12±425.96, P＞0.05). ConclusionsThe increased frequency of KIR2DS1 may be one of the reasons for URSA． A genetic imbalance between activating and inhibitory KIR genes might confer susceptibility to the occurrence of abortion.

Key words: Killerimmunoglobulinlike receptor; Recurrent spontaneous abortion; Decidua; Gene

中图分类号:

R714.12

徐金娥1,2，王珊1，赵跃然1，陈子江1. 蜕膜组织杀伤细胞免疫球蛋白样受体与不明原因早期复发性流产的相关性[J]. 山东大学学报(医学版), 2009, 47(12): 81-85.

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