滤纸干血片法筛查孕中期唐氏综合征的可行性

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (12): 86-90.

滤纸干血片法筛查孕中期唐氏综合征的可行性

刘霞^1,2 ,周玉侠^1,2，张莉³，范刚¹，孙萍^1,2，沈峰^1,2

1. 山东大学省立医院妇产医院，济南 250014； 2. 山东省妇幼保健所，济南 250014；
3. 上海交通大学附属上海市儿童医院，上海 200040

收稿日期:2009-08-25 出版日期:2009-12-16 发布日期:2009-12-16
作者简介:刘霞（1959- ），女，主任医师，主要从事新生儿疾病的研究。 Email:Lxbj2006@yahoo.com.cn
基金资助:
2006年山东省医药卫生科研项目（第88号）。

Feasibility of the dried blood specimen method to screen prenatal Down′s syndrome among midterm pregnant women

LIU Xia ^1,2 , ZHOU Yuxia ^1,2 , ZHANG Li ³ , FAN Gang ¹ , SUN Ping ^1,2，SHEN Feng ^1,2

1. Hospital of Gynecology and Obstetrics, Provincial Hospital Affiliated to Shandong University, Jinan 250014, China;
2. Shandong Provincial Maternal and Child Health Institute, Jinan 250014, China;
3. Shanghai Children′s Hospital Affiliated to Shanghai Jiaotong University, Shanghai 200040, China

Received:2009-08-25 Online:2009-12-16 Published:2009-12-16

摘要/Abstract

摘要：

目的探讨滤纸干血片法应用于孕中期妇女唐氏(Down′s)综合征产前筛查的可行性。方法应用自动时间分辨荧光免疫分析(Auto DELFIA)法检测滤纸干血片洗脱液中血清甲胎蛋白(AFP)和游离β人绒毛膜促腺性激素（游离βhCG）的含量，并对干血片检测方法的精密度、回收率、检测限等进行评估。结果该方法检测AFP水平的变异度为2.5%～6.8%；回收率为95.1%；检测低限为0.5?u/mL；检测游离βhCG水平的变异度为4.1%～7.0%；回收率为97.6%；检测低限为1?μg/L；发病风险为1/128，本研究共测定孕妇1?320例，筛查出阳性病例85例，筛查阳性率为6.4%，经羊水染色体检查确诊1例Down′s综合征胎儿。与国际公认的金标准血清学筛查结果的符合率为98.4%。结论采用滤纸干血片法替代血清学方法测定孕中期女性血中AFP和游离β hCG的含量，用血量少，方便快捷可行，不增加筛查成本，筛查结果准确可靠。

关键词: 滤纸干血片；唐氏综合征；甲胎蛋白；游离β人绒毛膜促腺性激素

Abstract:

To explore the feasibility of the dried blood specimen methods to screen Down's syndrome among midterm pregnant women. MethodsThe levels of serum Alphafetoprotein（AFP）and free betahuman chorionic gomadotropi (βhCG) in dried blood specimens on filter paper were detected with the method of timeresolved fluoroimmunoassay. The variation coefficient, recovery rate and detection limit were estimated. ResultsWhen the method was used to test AFP， the variation coefficient, recovery rate and detection limit were 2.5%64.8%, 95.1% and 0.5?u/mL respectively. When the method was used to test βhCG, the corresponding results were 4.1%7.0%, 97.6% and 1?U/mL respectively. The onset risk rate was 1/128, with an accordance rate of 98.4% compared with that of the gold standard of serum screening method which is universally accepted. A total of 1?320 pregnant women were tested in this study, and 85 positive cases(6.4% )were screened out. One fetus was diagnosed as having Down′s syndrome by detecting the chromosomes in the amniotic fluid. ConclusionsThe method of timeresolved fluoroimmunoassay through dried blood specimen, in place of the serum method, to detect the levels of AFP and βhCG among midterm pregnant women is convenient and feasible and its screening result is accurate and reliable.

Key words: Dried blood spots specimen on filter paper; Down′s syndrome; Alpha fetoprotein; Free betahuman chorionic gomadotropi

中图分类号:

R714.5

刘霞1,2,周玉侠1,2，张莉3，范刚1，孙萍1,2，沈峰1,2. 滤纸干血片法筛查孕中期唐氏综合征的可行性[J]. 山东大学学报(医学版), 2009, 47(12): 86-90.

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