喜树碱Pluronic P105 /L61 混合胶束系统的制备

山东大学学报(医学版) ›› 2009, Vol. 47 ›› Issue (11): 128-131.

喜树碱Pluronic P105 /L61 混合胶束系统的制备

孙新堂，王金丽，李凌冰

山东大学药学院药剂教研室，济南 250012

收稿日期:2009-04-24 发布日期:2009-11-16
通讯作者: 李凌冰（1964- ）,男，教授，主要从事纳米靶向制剂的研究。 Tel：15865252292, Email：cnlbli@sdu.edu.cn
作者简介:孙新堂(1975- ),男, 硕士研究生,主要从事纳米制剂的研究。
基金资助:
山东省自然科学基金项目（Y2005C65）。

Mixed micelles made of Pluronic P105 and L61 as pharmaceutical nanocarriers for camptothecin

SUN Xintang， WANG Jinli， LI Lingbing

Department of Pharmaceutics, School of Pharmacy, Shandong University, Jinan 250012, China

Received:2009-04-24 Published:2009-11-16

摘要/Abstract

摘要：

目的制备载有喜树碱的混合胶束系统，增加喜树碱的载药量。方法以Pluronic P105（P105）和Pluronic L61(L61)为材料,难溶性抗癌药物喜树碱为模型药物, 制备了载药混合胶束系统。对混合胶束系统的载药量、稳定性进行了研究。采用人胸腺癌细胞系(MCF7), 对载药混合胶束的细胞毒性进行了研究。结果所制备的混合胶束系统,长期存放和多倍稀释后仍然能够稳定存在；加入L61后喜树碱的载药量明显提高。细胞毒性实验结果表明,混合胶束对人胸腺癌细胞系(MCF7)的细胞毒性高于原料药。结论Pluronic P105 /L61 混合胶束系统是一种比较稳定的喜树碱给药系统。

关键词: 胶束;喜树碱;细胞毒性

Abstract:

To increase the drug loading of the poorly soluble anticancer drug, camptothecin (CPT)， micelles made of a mixture of Pluronic P105 (P105) and Pluronic L61 (L61) were prepared. MethodsMixed micelles made of a mixture of Pluronic P105 (P105) and Pluronic L61 (L61) were prepared loaded with CPT. The drug loading and colloidal stability of such systems were studied, and cytotoxicity of the CPTloaded mixed micelles against MCF7 cancer cells in vitro was determined. ResultsThe CPTloaded mixed micelles were stable upon storage and dilution. Drug loading of CPT was increased with an increase of the amount of Pluronic L61. Cytotoxicity of the CPTloaded mixed micelles against MCF7 cancer cells in vitro was remarkably higher than that of the free drug. ConclusionMixed micelles made of a mixture of Pluronic P105 (P105) and Pluronic L61 (L61) may serve as a stable delivery system for poorly soluble CPT.

Key words: Pluronic micelles; Camptothecin; Cytotoxicity

中图分类号:

R944.9

孙新堂，王金丽，李凌冰. 喜树碱Pluronic P105 /L61 混合胶束系统的制备[J]. 山东大学学报(医学版), 2009, 47(11): 128-131.

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