hCG对HTR8SVneo滋养层细胞系MMP2表达的影响与调控机制

山东大学学报(医学版)

hCG对HTR8SVneo滋养层细胞系MMP2表达的
影响与调控机制

李泽武¹，杨美香²，孙锦堂²，解奇²，邓碧萍²，刘佳²，宋丙凤²，毛海婷²，曲迅²

(1. 山东省医学科学院基础医学研究所，济南 250062； 2. 山东大学齐鲁医院临床基础研究所，济南 250012)

收稿日期:1900-01-01 修回日期:1900-01-01 发布日期:2009-04-16
通讯作者: 曲迅

Effects of hCG on invasive activity of HTR8SVneo trophoblastic
cells and its mechanism

LI Zewu¹, YANG Meixiang², SUN Jintang², XIE Qi², DENG Biping²,
LIU Jia²,　SONG Bingfeng², MAO Haiting², QU Xun²

(1. Institute of Basic Medical Sciences, Shandong Academy of Medical Sciences, Jinan 250062, China;
2. Basic Medical Science Institute，Qilu Hospital of Shandong University， Jinan 250012， China)

Received:1900-01-01 Revised:1900-01-01 Published:2009-04-16
Contact: QU Xun

摘要/Abstract

摘要： 目的探讨绒毛膜促性腺激素（hCG）对滋养层细胞系HTR8SVneo侵袭活性的影响及其可能的调控机制。方法将培养的HTR8SVneo细胞根据处理因素分为对照组、低浓度hCG（10?IU/mL）刺激组、高浓度hCG（100?IU/mL）刺激组，再将HTR8SVneo细胞分为对照组、hCG（100?IU/mL）刺激组、DPCPX(A1R特异性阻断剂）+hCG（100IU/mL）联合刺激组。采用RTPCR检测各组HTR8SVneo细胞基质金属蛋白酶2（MMP2）的表达变化，明胶酶谱检测各组HTR8SVneo细胞MMP2蛋白水平的分泌变化。结果高浓度hCG（100?IU/mL）较低浓度hCG（10?IU/mL）刺激后的HTR8SVneo细胞MMP2的表达与分泌均上升（P<均0.05）；腺苷受体A1R的表达同时升高（P<0.05）。阻断A1R的作用通路后，MMP2的表达与分泌均下降（P均<0.05）。结论随着hCG浓度的升高， HTR8SVneo细胞的侵袭性逐步增强，其调控机制为通过促进A1R的表达而使MMP2的分泌升高。

关键词: 滋养层细胞, 人绒毛膜促性腺激素, 腺苷受体, 基质金属蛋白酶2

Abstract: To study the effect of hCG on invasion of HTR8SVneo cell line and to explore its mechanism. MethodsFirst, cultured HTR8SVneo cells were divided into the control group, the low concentration of hCG (10?IU/mL) to stimulate group, and the high concentrations of hCG (100?IU/mL) to stimulate group, then for further experiments they were divided into the control group, the hCG (100?IU/mL) stimulation group, and the DPCPX (A1R specific blocker) +hCG (100?IU/mL) joint stimulation group. The transcription levels of MMP2 in HTR8SVneo cells were determined by RTPCR, and the secreting protein trend of MMP2 was determined by gelatin mass spectrometry. ResultsProduction of MMP2 protein was consistent with levels of MMP2 mRNA treated with different concentration hCG. Expression of A1R was increased at high concentration (100?IU/mL), however, levels of MMP2 mRNA and its secreted protein were reduced after blocking the A1R by DPCPX. ConclusionhCG promotes HTR8SVneo cell invasion by increasing expression of A1R.

Key words: Trophoblastic cell, Human chorionic gonadotropin, Adenosine receptor, Matrix Metalloproteinase

中图分类号:

R714.12

李泽武1 ，杨美香2 ，孙锦堂2 ，解奇2 ，邓碧萍2 ，刘佳2 ，宋丙凤2 ，毛海婷2 ，曲迅2
. hCG对HTR8SVneo滋养层细胞系MMP2表达的
影响与调控机制[J]. 山东大学学报(医学版), 2009, 47(04): 18-20.

LI Zewu¹, YANG Meixiang², SUN Jintang², XIE Qi², DENG Biping²,
LIU Jia²,　SONG Bingfeng², MAO Haiting², QU Xun²
. Effects of hCG on invasive activity of HTR8SVneo trophoblastic
cells and its mechanism [J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2009, 47(04): 18-20.

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