人参总皂甙对缺血性大鼠模型再灌注损伤后脑细胞凋亡的影响

山东大学学报(医学版)

人参总皂甙对缺血性大鼠模型再灌注损伤后脑细胞凋亡的影响

张东君¹,李义召^2，3,吴晓燕⁴,张振¹

1. 山东大学齐鲁医院神经内科, 济南 250012；2. 山东省残疾人康复中心，济南 250010；3. 山东大学公共卫生学院毒理学研究所，济南 250012；4. 山东省菏泽市人民医院神经内科，山东菏泽 274000

收稿日期:2007-09-02 修回日期:1900-01-01 出版日期:2007-12-24 发布日期:2007-12-24
通讯作者: 张东君

Protective effect of ginseng total saponin on the neuroal injury in rat models with focal cerebral ischemic reperfusion

ZHANG Dong-jun¹,LI Yi-zhao^2，3,WU Xiao-yan⁴,ZHANG Zhen¹

1. Department of Neurology, Qilu Hospital of Shandong University；2. Disabled Rehabilitation Center, Shandong Province；3. Institute of Toxicology, School of Public Health, Shandong University；4. Department of Neurology, Heze People′s Hospital

Received:2007-09-02 Revised:1900-01-01 Online:2007-12-24 Published:2007-12-24
Contact: ZHANG Dong-jun

摘要/Abstract

摘要： 目的利用大鼠脑缺血再灌注模型，观察人参总皂甙对缺血再灌注后脑细胞凋亡的影响，探讨人参总皂甙对大鼠缺血再灌注脑组织的保护作用机制。方法将健康成年Wistar大鼠40只随机分为5组，即正常对照组、假手术组、脑缺血再灌注非治疗组、缺血再灌注人参总皂甙治疗1次组(治疗组1)、缺血再灌注人参总皂甙治疗连用3d组(治疗组2)。治疗组1于再灌注开始前，经腹腔注入人参总皂甙(50mg／Kg)1次,48h后断头取脑；治疗组2连续3d腹腔注射人参总皂甙(50mg／Kg)，72h后断头取脑；缺血再灌注非治疗组和假手术组给予等量生理盐水腹腔注射，48h后断头取脑。石蜡切片行Nissel、TUNEL染色，计算凋亡细胞数，同时进行神经功能评价。结果TUNEL染色显示缺血非治疗组神经细胞数量明显减少，海马CA1区可见较多的散在的TUNEL染色阳性细胞。治疗组仅见少量神经细胞变性，胞体变形缩小。缺血再灌注非治疗组与假手术组比较凋亡细胞数量明显增多，P<0.01；治疗组1、治疗组2与缺血再灌注非治疗组比较凋亡细胞均明显减少，P<0.01；治疗组2与治疗组l比较凋亡细胞亦明显减少，P<0.01。治疗组1、治疗组2分别与缺血再灌注非治疗组比较，神经功能评分显著提高，P<0.01，治疗组2与治疗组l相比神经功能评分提高，P<0.05，均有统计学意义。结论人参总皂甙能明显抑制局灶性脑缺血再灌注大鼠海马CA1区神经细胞的凋亡，显著提高大鼠的神经功能评分，具有明显的神经营养和保护作用。

关键词: 人参总皂甙 , 细胞凋亡, 再灌注损伤

Abstract: Objective To investigate the protective effects of ginseng total saponin on brain tissue injury following ischemiareperfusion in rats with brain ischemia-reperfusion. Methods40 adult male Wistar rats were randomly divided into 5 groups: the control, the sham, the ischemic, the ginseng total saponintreated 1, and the ginseng total saponin-treated 2 groups. The rats received either ginseng total saponin (50mg/kg of body weight.) or saline i.p. just before the beginning of reperfusion and the brains were removed after 48h, and the ginseng total saponintreated 2 group was subjected to ginseng total saponin for 3 days and their brains were removed after 72?h. The brain tissues were observed by Nissle staining and TUNEL staining, and the data were calculated by using the image analysis system. ResultsIn the ischemic group, the number of neurocyte cells obviously decreased. TUNELpositive cells were not found in the normal rats but were found in the brain tissues of the sham and the ginseng total saponintreated groups. However, TUNEL-positive cells were found in the ischemic group. Deep brown staining was found in the nuclei of hippocampal and cortical neurons. Ginseng total saponin resulted in significantly fewer TUNELpositive cells in the neurons compared with the ischemic group (P<0.01). Both the and 2 groups had higher neuronal function values than the ischemic reperfusion nontreatment group (P<0.01), and the ginseng total saponin-treated 2 group was superior to the 1 group (P<0.05). ConclusionsGinseng total saponin may restrain the apoptosis of hippocampal neurons and increases the neuronal function value of focal cerebral ischemicreperfusion rats, and well protects the neurons from ischemiainduced apoptosis.

Key words: Reperfusion injury, Apoptosis, Ginseng total saponin

中图分类号:

R743

张东君,李义召,吴晓燕,张振 . 人参总皂甙对缺血性大鼠模型再灌注损伤后脑细胞凋亡的影响[J]. 山东大学学报(医学版), 2007, 45(12): 1247-1252.

ZHANG Dong-jun,LI Yi-zhao,WU Xiao-yan,ZHANG Zhen. Protective effect of ginseng total saponin on the neuroal injury in rat models with focal cerebral ischemic reperfusion[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(12): 1247-1252.

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