关键词: 肿瘤坏死因子-ａ , 心搏骤停, 核转录因子-κB, 生脉注射液

Abstract: ObjectiveTo observe the effects of Shengmai Injection on NF-κB activation of the neutrophile granulocyte, serum TNF-a level and the prognosis of resuscitation on patients who had recovered from cardiac arrest. MethodsThe enzyme labeled immunohistochemical method was used to determine the NF-κB activation degree. Radio-immunity (RI) was used to determine the blood serum level of TNF-a. Changes of vital signs and laboratory examination indexes were also observed. Results①The time, degree of vital signs recovery of the test group were superior to those of the control group, and the differences were statistically significant (P＜0.05). ② The degree that NF-κB moved into the nucleolus and the TNF-a level of the control group were higher than those of the test group, and the differences were statistically significant (P＜0.01). ③Shengmai Injection suppressed the activation of NF-κB and the blood serum level of TNF-a on dosage (P＜0.01). ④ The NF-κB activation degree and the TNF-a level were negatively correlated with the recovery of vital signs. The better the vital signs recovered, the lower were the NF-KB activation and the TNF-a level. ConclusionShengmai Injection plus routine therapeutics have obvious therapeutic efficiency in protecting heart function, supporting good circulation state, and increasing the success rate of CPR possibly by suppressing the NF-κB signaling pathway and reducing NF-κB controled inflammation factors in a dose-dependent manner.

Key words: Shengmai Injection, Sudden cardiac arrest, Nuclear factor-kappaB, Tumor necrosis factor-alpha