葛根素对球囊损伤后再狭窄过程中组织蛋白酶S及胱蛋白C表达的影响

山东大学学报(医学版)

葛根素对球囊损伤后再狭窄过程中组织蛋白酶S及胱蛋白C表达的影响

王群，鹿庆华，蒋卫东，刘玉胜，杜贻萌，董兆强，王欣，郝琳，秦爱琼，徐冬玲，盛林

山东大学第二医院心内科，济南 250033

收稿日期:2007-08-30 修回日期:1900-01-01 出版日期:2007-11-24 发布日期:2007-11-24
通讯作者: 鹿庆华

Effect of Puerarin on Cathepsin S and Cystatin C during the procedure of vascular restenosis in balloon injured rabbits

WANG Qun, LU Qing-hua, JIANG Wei-dong, LIU Yu-sheng, DU Yi-meng, DONG Zhao-qiang, WANG Xin,HAO Lin, QIN Ai-qiong, XU Dong-ling, SHENG Lin

Department of Cardiovascular Disease, Second Hospital of Shandong University, Jinan 250033, China

Received:2007-08-30 Revised:1900-01-01 Online:2007-11-24 Published:2007-11-24
Contact: LU Qing-hua

摘要/Abstract

摘要： 目的研究葛根素对动脉球囊损伤后再狭窄成过程中组织蛋白酶S（cathepsin S，CatS）及其抑制剂胱蛋白C（cystatin C,CysC）表达的影响。方法建立兔髂－腹主动脉球囊损伤后再狭窄模型，观察应用葛根素治疗前后再狭窄形成的范围、再狭窄局部CatS及其抑制剂CysC的表达变化以及内皮下胶原的变化。结果葛根素组［100mg/(kg·d)］与球囊损伤组比较，再狭窄范围明显减小，差异具有统计学意义（P<0.05）；CatS的表达水平减低，差异具有统计学意义（P<0.05）；CysC表达水平差异无统计学意义（P>0.05）；内皮下胶原含量减少，差异具有统计学意义（P<0.01）。结论葛根素可降低球囊损伤部位再狭窄形成过程中CatS表达、减少斑块内胶原的聚集而发挥一定的抑制再狭窄形成作用，但对CysC调节作用不显著。

关键词: 胱蛋白C , 组织蛋白酶S, 再狭窄, 基质重塑, 葛根素

Abstract: ObjectiveTo explore the effects of Puerarin on Cathepsin S and Cystatin C during the procedure of vascular restenosis after balloon injury. MethodsThe experimental models of vascular restenosis in rabbits were established by anendothelial injury induced by a balloon and hyper-lipid feed. The arterious restenosis area, expressions of Cathepsin S and Cystatin C, and the differences of collagen were determined. ResultsAt the end of 8 weeks after an endothelial injury by a balloon, the restenosis area in the Puerarin group(100mg/kg per day) was obviously smaller than that in the balloon injured group（P<0.05）. The expression of Cathepsin S in the Puerarin group was lighter than that in the balloon injured group (P<0.05), however the expression of Cystatin C was not significantly different between the Puerarin group and the balloon injured group (P>0.05). Collagen in the intima was significantly decreased in the Puerarin group compared with that in the balloon injured group (P<0.01). ConclusionPuerarin has a regressive effect on vascular restenosis in rabbits by modulating the expression of Cathepsin S and reducing collagen accumulation in the vascular restenosis area, but it does not affect the expression of Cystatin C.

Key words: Puerarin, Restenosis, Matrix remodeling, Cathepsin S, Cystatin C

中图分类号:

R541.4

王群,鹿庆华,蒋卫东,刘玉胜,杜贻萌,董兆强,王欣,郝琳,秦爱琼,徐冬玲,盛林. 葛根素对球囊损伤后再狭窄过程中组织蛋白酶S及胱蛋白C表达的影响[J]. 山东大学学报(医学版), 2007, 45(11): 1122-1126.

WANG Qun,LU Qing-hua,JIANG Wei-dong,LIU Yu-sheng,DU Yi-meng,DONG Zhao-qiang,WANG Xin,HAO Lin,QIN Ai-qiong,XU Dong-ling,SHENG Lin. Effect of Puerarin on Cathepsin S and Cystatin C during the procedure of vascular restenosis in balloon injured rabbits[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(11): 1122-1126.

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