柞蚕雄蛾提取液改善W256荷瘤大鼠放疗后免疫功能的实验研究

山东大学学报(医学版)

柞蚕雄蛾提取液改善W256荷瘤大鼠放疗后免疫功能的实验研究

张维东¹，张月英¹，王朝霞¹，贾青¹，王兆朋¹，于金明²

1．山东省医学科学院基础医学研究所山东省现代医用药物与技术重点实验室，济南 250062；2．山东省肿瘤防治研究院，济南 250117

收稿日期:2007-05-16 修回日期:1900-01-01 出版日期:2007-11-24 发布日期:2007-11-24
通讯作者: 于金明

Effect of an extract from the male zooid of Antheraea pernyi on reversing radiation-induced immunosuppression in rats bearing W256 carcinoma

ZHANG Wei-dong¹，ZHANG Yue-ying¹，WANG Zhao-xia¹，JIA Qing¹，WANG Zhao-peng¹，YU Jin-ming²

1. Institute of Basic Medicine, Shandong Academy of Medical Science, Key Laboratory for Modern Medicine and Technology of Shandong Province, Jinan 250062, China；2. Shandong Provincial Tumor Hospital, Jinan 250117

Received:2007-05-16 Revised:1900-01-01 Online:2007-11-24 Published:2007-11-24
Contact: YU Jin-ming

摘要/Abstract

摘要： 摘要：目的观察柞蚕雄蛾提取液对荷W256癌肉瘤大鼠放疗后免疫功能的影响。方法60只Wistar大鼠随机分为正常对照组、模型组、单纯放疗组和实验组（放疗与柞蚕蛾提取液联合治疗组），每组15只。建立W256荷瘤大鼠模型，分离脾脏和胸腺，并计算脏器指数；采用流式细胞术（FCM）及酶联免疫吸附试验（ELISA）分别检测外周血T淋巴细胞亚群变化和血清中细胞因子INF-γ和IL-4含量。结果与放疗组相比较，柞蚕雄蛾提取液可以升高放疗后荷瘤大鼠脾脏和胸腺指数，增加放疗后荷瘤大鼠外周血中CD4⁺T细胞比例与CD4⁺T/CD8⁺T值（P＜0.05）；实验组荷瘤大鼠血清中细胞因子INF-γ含量明显升高，而IL-4含量显著降低（P＜0.05）。结论柞蚕雄蛾提取液可以逆转荷瘤大鼠放疗后免疫功能抑制状态，改变荷瘤机体免疫功能，可作为恶性肿瘤传统放疗中的辅助用药。

关键词: Wistar , T淋巴细胞亚群, 大鼠, Th1/Th2漂移, 柞蚕

Abstract: Abstract: ObjectiveTo study the effects of an extract from the male zooid on reversing the radiation-induced immunosuppression of rats bearing W256 carcinoma. MethodsTumor models were established by subcutaneously injecting W256 tumor cells into rats. 60 Wistar rats were randomly divided into 4 groups. The thymus index and the spleen index were used as parameters to evaluate the effect of an extract from the male zooid on non-specific and specific immune function of rats with tumor. The presence of Interferon gamma (INF-γ) and interleukin-4 (IL-4) in the peripheral blood serum were screened by enzyme-linked immunosorbent assay (ELISA) to investigate cell-mediated immune responses and T subsets were determined by flow cytometry(FCM). ResultsThe extract from the male zooid significantly raised the thymus index and the spleen index of rats with tumors. The number of CD4⁺T and the CD4⁺T/CD8⁺T ratio of W256-bearing rats treated with the extract from the male zooid significantly increased compared with the radiotherapy group (P＜0.05). Also, the level of Interferon-gamma (INF-γ) was up-regulated and interleukin-4(IL-4) was down-regulated. ConclusionsThe extract from the male zooid of Antheraea pernyi has effect of reversing the radiation-induced immunosuppression and could be regarded as a main assistant agent combined with traditional radiotherapy for treatment of malignant tumors.

Key words: Antheraea pernyi, Th1/Th2 shift, T-lymphocyte Subsets, Rat, Wistar

中图分类号:

R979.1

张维东,张月英,王朝霞,贾青,王兆朋,于金明 . 柞蚕雄蛾提取液改善W256荷瘤大鼠放疗后免疫功能的实验研究[J]. 山东大学学报(医学版), 2007, 45(11): 1085-1087.

ZHANG Wei-dong,ZHANG Yue-ying,WANG Zhao-xia,JIA Qing,WANG Zhao-peng,YU Jin-ming. Effect of an extract from the male zooid of Antheraea pernyi on reversing radiation-induced immunosuppression in rats bearing W256 carcinoma[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2007, 45(11): 1085-1087.

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