α5-nAChR对慢性应激肺腺癌荷瘤小鼠疑核c-Fos表达的影响

doi:10.6040/j.issn.1671-7554.0.2024.0717

摘要/Abstract

摘要： 目的探讨α5-烟碱型乙酰胆碱受体(α5-nicotinic acetylcholine receptor, α5-nAChR)对慢性不可预见性应激(chronic unpredictable mild stress, CUMS)肺腺癌荷瘤小鼠疑核c-Fos表达的影响。方法运用免疫组织化学技术检测NC+CUMS组(n=6)、CUMS+CHRNA5^+/+组(n=6)及CUMS+CHRNA5^+/++Mec组(n=6)肺腺癌荷瘤小鼠瘤组织中α5-nAChR与Ki67的表达及相关性;运用免疫荧光技术检测NC组(n=6)、NC+CUMS组(n=6)、CUMS+CHRNA5^+/+组(n=6)及CUMS+CHRNA5^+/++Mec组(n=6)肺腺癌荷瘤小鼠疑核c-Fos的表达。结果 α5-nAChR与Ki67的表达呈正相关(P<0.05),α5-nAChR介导慢性应激促进肺腺癌细胞增殖;α5-nAChR表达与疑核c-Fos的表达呈正相关(P<0.05)。结论 α5-nAChR调控慢性应激肺腺癌荷瘤小鼠疑核c-Fos的表达。

关键词: α5-烟碱型乙酰胆碱受体, c-Fos, 疑核, 慢性应激, 肺腺癌, 小鼠

Abstract: Objective To investigate the effect of α5-nicotinic acetylcholine receptor(α5-nAChR)on the expression of c-Fos in nucleus ambiguus in chronic unpredictable mild stress(CUMS)-induced lung adenocarcinoma mice. Methods The expression and correlation between α5-nAChR and Ki67 in tumour tissues of the NC+CUMS group(n=6), the CUMS+CHRNA5^+/+ group(n=6)and the CUMS+CHRNA5^+/++Mec group(n=6)were examined using immunohistochemistry in lung adenocarcinoma mice. Immunofluorescence technique was used to detect the expression of c-Fos in nucleus ambiguus of the NC group(n=6), the NC+CUMS group(n=6), the CUMS+CHRNA5^+/+ group and the CUMS+CHRNA5^+/++Mec group(n=6)in lung adenocarcinoma mice. Results α5-nAChR was positively correlated with the expression of Ki67(P<0.05)and α5-nAChR mediates chronic stress-induced lung adenocarcinoma cell proliferation. α5-nAChR expression was positively correlated with the expression of c-Fos in nucleus ambiguus(P<0.05). Conclusion α5-nAChR mediates the expression of c-Fos in nucleus ambiguus in chronic stress-induced lung adenocarcinoma mice.

Key words: α5-nicotinic acetylcholine receptor, c-Fos, Nucleus ambiguus, Chronic stress, Lung adenocarcinoma, Mice

中图分类号:

R734.2

蔡佳莹,王靖婷,王增萍,王璟,郏雁飞,马晓丽. α5-nAChR对慢性应激肺腺癌荷瘤小鼠疑核c-Fos表达的影响[J]. 山东大学学报 (医学版), 2025, 63(4): 69-74.

CAI Jiaying, WANG Jingting, WANG Zengping, WANG Jing, JIA Yanfei, MA Xiaoli. Effect of α5-nAChR on the expression of c-Fos in nucleus ambiguus in chronic stress-induced lung adenocarcinoma mice[J]. Journal of Shandong University (Health Sciences), 2025, 63(4): 69-74.

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