您的位置：山东大学 -> 科技期刊社 -> 《山东大学学报（医学版）》

山东大学学报 (医学版) ›› 2023, Vol. 61 ›› Issue (5): 90-95.doi: 10.6040/j.issn.1671-7554.0.2023.0027

• 公共卫生与管理学 • 上一篇    

2021年济宁市柯萨奇病毒A16型流行特点及全基因组特征

王珊1,2,焦伯延2,冯强3,段延华2,温红玲1   

  1. 1.山东大学公共卫生学院, 山东 济南 250012;2.济宁市疾病预防控制中心, 山东 济宁 272000;3.济宁市任城区疾病预防控制中心, 山东 济宁 272000
  • 发布日期:2023-05-15
  • 通讯作者: 温红玲. E-mail:wenhongling@sdu.edu.cn
  • 基金资助:
    山东省医药卫生科技发展计划项目(202112060725);山东省学校卫生协会科研课题(SDWS2022046)

Epidemic and whole genome characteristics of coxsackievirus A16 in Jining in 2021

WANG Shan1,2, JIAO Boyan2, FENG Qiang3, DUAN Yanhua2, WEN Hongling1   

  1. 1. School of Public Health, Shandong University, Jinan 250012, Shandong, China;
    2. Jining Center for Disease Control and Prevention, Jining 272000, Shandong, China;
    3. Renchen Center for Disease Control and Prevention, Jining 272000, Shandong, China
  • Published:2023-05-15

PDF (PC)

63

摘要： 目的 了解济宁市2021年柯萨奇病毒A16 型(CV-A16)流行特点和全基因组特征。 方法 2021年1月至12月采集济宁市疑似手足口病患者粪便标本513份,进行CV-A16型病毒核酸检测和病毒分离,对分离的4株CV-A16进行全基因组测序和生物信息学分析。 结果 共检出CV-A16型36例,阳性率为7.02%(36/513),其中7~9月阳性率最高。4株毒株VP1基因位于B1a和B1b两个进化分支。与原型株G-10(CAU05876)相比,4株毒株核苷酸和氨基酸同源性分别为77.5%~78.0%和94.7%~94.9%。4株毒株编码蛋白均发生多处氨基酸变异。 结论 CV-A16是2021年引起济宁市手足口病的主要病原体之一,在济宁市流行的主要是B1a和B1b两个亚型,需要加强手足口病监测力度。

关键词: 手足口病, 柯萨奇病毒A16, 全基因组测序

Abstract: Objective To analyze the epidemic and whole genome characteristics of coxsackievirus A16(CV-A16)in Jining in 2021. Methods A total of 513 stool samples were collected from patients with hand, foot and mouth disease(HFMD), which were then underwent nucleic acid detection of CV-A16 and virus isolation. Whole genome sequencing and bioinformatics analysis were performed on 4 CV-A16 strains. Results Altogether 36 cases were positive for CV-A16, with a positive rate of 7.02%(36/513). The positive rate was the highest from July to September. The 4 strains were distributed in B1a and B1b branches. Compared with prototype strain G-10(CAU05876), the 4 strains had homology of nucleotide and amino acid of 77.5%-78.0% and 94.7%-94.9%, respectively. There were multiple amino acid changes in the encoded proteins of the 4 strains. Conclusion CV-A16 was one of the main pathogens of HFMD in Jining in 2021, including B1a and B1b subtypes. The surveillance of the disease should be strengthened.

Key words: Hand, foot and mouth disease, Coxsackievirus A16, Whole genome sequencing

中图分类号: 

  • R373.2
[1] Chen W, Li J, Li J, et al. Roles of non-coding RNAs in virus-host interaction about pathogenesis of hand-foot-mouth disease [J]. Curr Microbiol, 2022, 79(9): 247. doi: 10.1007/s00284-022-02928-z.
[2] Chen Z, Jiang C, Cheng X, et al. Secondary hypogonadism following hand, foot, and mouth disease in an adult: a case report and review of literature [J]. BMC Infect Dis, 2022, 22(1): 56. doi: 10.1186/s12879-022-07030-0.
[3] 黄浩, 盘珍梅, 潘燕兰, 等. 致手足口病肠道病毒的病原学研究进展[J]. 病毒学报, 2021, 37(6): 1491-1501. HUANG Hao, PAN Zhenmei, PAN Yanlan, et al. Research progress on the etiology of enterovirus causing hand foot and mouth disease [J]. Chinese Journal of Virology, 2021, 37(6): 1491-1501.
[4] 张静, 李秀惠, 李丽, 等. 手足口病病原学和流行病学研究进展[J]. 中华流行病学杂志, 2022, 43(5): 771-783. ZHANG Jing, LI Xiuhui, LI Li, et al. Etiology and epidemiology of hand, foot and mouth disease in China [J]. Chinese Journal of Epidemiology, 2022, 43(5): 771-783.
[5] Fang CY, Liu CC. Recent development of enterovirus A vaccine candidates for the prevention of hand, foot, and mouth disease [J]. Expert Rev Vaccines, 2018, 17(9): 819-831.
[6] 张斯钰, 董瑞, 赵善露, 等. 2019—2020年湖南省手足口病流行特征和病原学特征研究[J]. 实用预防医学, 2022, 29(1): 22-26. ZHANG Siyu, DONG Rui, ZHAO Shanlu, et al. Epidemic and etiological characteristics of hand-foot-mouth disease in Hunan Province, 2019—2020 [J]. Practical Preventive Medicine, 2022, 29(1): 22-26.
[7] 秦连阳,尹保行,连颜芳,等. 2009—2018年山西省临汾市手足口病流行病学调查分析[J]. 中国卫生监督杂志, 2020, 27(6): 576-580. QIN Lianyang, YIN Baohang, LIAN Yanfang, et al. Epidemiological analysis of hand, foot and mouth disease in Linfen, 2009—2018 [J]. Chinese Journal of Health Inspection, 2020, 27(6): 576-580.
[8] Li P, Chen Y, Tang A, et al. Seroprevalence of coxsackievirus A16 antibody among people of various age groups: a systematic review and meta-analysis [J]. Arch Public Health, 2021, 79(1): 166. doi: 10.1186/s13690-021-00688-z.
[9] 段延华. 2010—2016年济宁市手足口病流行病学特征分析[J]. 预防医学论坛, 2019, 25(7): 514-517. DUAN Yanhua. Analysis on epidemiological characteristics of hand foot and mouth disease, Jining City, 2010—2016 [J]. Preventive Medicine Tribune, 2019, 25(7): 514-517.
[10] 姜静静, 于兴浩, 颜丙新, 等. 2014—2018年济宁市手足口病流行特征及病原学分析[J]. 医学动物防制, 2021, 37(1): 79-81. JIANG Jingjing, YU Xinghao, YAN Bingxin, et al. Epidemiological characteristics and pathogenic analysis of hand-foot-mouth disease in Jining from 2014 to 2018 [J]. Journal of Medical Pest Control, 2021, 37(1): 79-81.
[11] 刘杨, 郭春艳, 张西嫔, 等. 柯萨奇病毒A16型VP1基因和病毒生物物理学分析[J]. 中华实验和临床感染病杂志(电子版), 2015, 9(2): 28-32. LIU Yang, GUO Chunyan, ZHANG Xipin, et al. Characterization of the VP1 region and biophysics of Coxsackievirus A16 [J]. Chinese Journal of Experimental and Clinical Infectious Diseases(Electronic Version), 2015, 9(2): 28-32.
[12] Mao Q, Wang Y, Yao X, et al. Coxsackievirus A16: epidemiology, diagnosis, and vaccine [J]. Hum Vaccin Immunother, 2014, 10(2): 360-367.
[13] Ding Y, Wang Z, Zhang X, et al. Different antibody response against the coxsackievirus A16 VP1 capsid protein: specific or non-specific [J]. PLoS One, 2016, 11(9): e0162820. doi: 10.1371/journal.pone.0162820.
[14] Shi J, Huang X, Liu Q, et al. Identification of conserved neutralizing linear epitopes within the VP1 protein of coxsackievirus A16 [J]. Vaccine, 2013, 31(17): 2130-2136.
[15] Guo J, Cao Z, Liu H, et al. Epidemiology of hand, foot, and mouth disease and the genetic characteristics of Coxsackievirus A16 in Taiyuan, Shanxi, China from 2010 to 2021 [J]. Front Cell Infect Microbiol, 2022, 12: 1040414. doi: 10.3389/fcimb.2022.1040414.
[16] Cheng D, Chiu YW, Huang SW, et al. Genetic and cross neutralization analyses of coxsackievirus A16 circulating in Taiwan from 1998 to 2021 suggest dominant genotype B1 can serve as vaccine candidate [J]. Viruses, 2022, 14(10): 2306. doi: 10.3390/v14102306.
[17] Mao Q, Wang Y, Yao X, et al. Coxsackievirus A16: epidemiology, diagnosis, and vaccine [J]. Hum Vaccin Immunother, 2014, 10(2): 360-367.
[18] 张丽芬, 周雪花, 徐敏, 等. 2019年云南省文山州柯萨奇病毒A16型的基因特征[J]. 昆明医科大学学报, 2020, 41(11): 18-24. ZHANG Lifen, ZHOU Xuehua, XU Min, et al. Genetic characteristic analysis of coxsackievirus A16 in Wenshan Prefecture of Yunnan Province in 2019 [J]. Journal of Kunming Medical University, 2020, 41(11): 18-24.
[19] Yi L, Zeng H, Zheng H, et al. Molecular surveillance of coxsackievirus A16 in southern China, 2008—2019 [J]. Arch Virol, 2021, 166(6): 1653-1659.
[20] Han Z, Song Y, Xiao J, et al. Genomic epidemiology of coxsackievirus A16 in mainland of China, 2000-18 [J]. Virus Evol, 2020, 6(2): veaa084. doi: 10.1093/ve/veaa084.
[21] 文婕, 王鹏飞, 李秀芳, 等. 2015年昆明市柯萨奇病毒A16型VP1区基因特征分析[J]. 中华疾病控制杂志, 2018, 22(6): 607-612. WEN Jie, WANG Pengfei, LI Xiufang, et al. Genetic characteristics analyses of the VP1 gene in coxsackievirus A16 isolates from Kunming in 2015 [J]. Chinese Journal of Disease Control & Prevention, 2018, 22(6): 607-612.
[22] 赵怀龙, 隋竑弢, 杨国樑, 等. 2017年济南市柯萨奇病毒A16型VP1基因特征分析[J]. 中华实验和临床病毒学杂志, 2019, 33(1): 44-48. ZHAO Huailong, SUI Hongtao, YANG Guoliang, et al. Characteristics of coxsackievirus A16 VP1 gene in Jinan, 2017 [J]. Chinese Journal of Experimental and Clinical Virology, 2019, 33(1): 44-48.
[23] Kumar R, Mehta D, Mishra N, et al. Role of host-mediated post-translational modifications(PTMs)in RNA virus pathogenesis [J]. Int J Mol Sci, 2020, 22(1): 323. doi: 10.3390/ijms22010323.
[24] Chamontin C, Bossis G, Nisole S, et al. Regulation of viral restriction by post-translational modifications [J]. Viruses, 2021, 13(11): 2197. doi: 10.3390/v13112197.
[25] Loboda AP, Soond SM, Piacentini M, et al. Lysine-specific post-translational modifications of proteins in the life cycle of viruses [J]. Cell Cycle, 2019, 18(17): 1995-2005.
[26] 徐莉. 中国手足口病中柯萨奇病毒A16流行株全基因组特征分析[D]. 杭州: 浙江大学, 2019.
[1] 冯一平,孙大鹏,王显军,纪伊曼,刘云霞. DLNM和LSTM神经网络对临沂市手足口病发病的预测效果比较[J]. 山东大学学报 (医学版), 2022, 60(2): 96-101.
[2] 关恒云,王春荣,刘岚铮,赵怀龙,白爱英,姜慧钰,董华,李娜,单晓英,成洪旗. 济南市2012~2017年手足口病4种优势肠道病毒阳性及VP1基因型分布[J]. 山东大学学报 (医学版), 2021, 59(5): 60-67.
[3] 关恒云,王春荣,刘岚铮,杨国樑,赵怀龙,孙洁,成洪旗. 济南市2012~2017年手足口病肠道病毒谱变化及流行特征[J]. 山东大学学报 (医学版), 2019, 57(11): 90-97.
[4] 刘言玉,刘志东,劳家辉,张静,姜宝法. 南宁市2008~2011年气象因素对手足口病的影响及其交互作用[J]. 山东大学学报 (医学版), 2018, 56(8): 95-100.
[5] 荀换苗,胡文琦,刘羿聪,马伟. 2009~2013年广东省热带气旋对手足口病的影响[J]. 山东大学学报 (医学版), 2018, 56(8): 50-55.
[6] 邓轲,刘毅,盖中涛. 间充质干细胞对肠道病毒71型的易感性及miRNA的差异表达[J]. 山东大学学报（医学版）, 2017, 55(3): 25-31.
[7] 刘红杨,肖金环,王凡,杨光,刘洪庆. 集中度和圆形分布法分析山东省2008~2014年手足口病季节性特征[J]. 山东大学学报（医学版）, 2016, 54(12): 77-81.
[8] 李敏, 段坤, 赵瑞霞. 2010-2013年济南市历城区414例手足口病病例分析[J]. 山东大学学报（医学版）, 2014, 52(9): 54-57.
[9] 关恒云, 王春荣, 吕燕，张雯，杨国樑，刘岚铮. 2012年济南市手足口病病原学及流行特征[J]. 山东大学学报(医学版), 2013, 51(10): 101-104.
[10] 袁晓晶1，温红玲1，高峰2，许洪芝1，司鲁莹1，王东旭1，宋艳艳1，赵丽1，王志玉1. 肠道病毒71型基因组5′非编码区序列分析[J]. 山东大学学报(医学版), 2012, 50(9): 127-.
[11] 岳盈盈1，张颖2，李鹏1，李志会1，宋楠楠1，纪璇1，盖中涛2，孟红1. [J]. 山东大学学报(医学版), 2012, 50(2): 106-109.
[12] 薛宁，王纪文,张洪伟. 大剂量甲泼尼龙冲击治疗重症手足口病的意义[J]. 山东大学学报(医学版), 2010, 48(3): 106-.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
版权所有 © 2018 《山东大学学报(医学版)》编辑部　
地址:山东大学科技期刊社（济南市历城区山大南路27号山东大学中心校区明德楼B座721室） 电话: 0531-88366918 E-mail: xbyxb@sdu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发