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山东大学学报 (医学版) ›› 2019, Vol. 57 ›› Issue (4): 15-19.doi: 10.6040/j.issn.1671-7554.0.2019.032

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自然杀伤细胞通过树突状细胞调控衣原体肺部感染中Th17/Treg免疫应答平衡

周小青1,李静1,2,赵蕾3,唐伟1,杨熙1,4,王红1   

  1. 1.山东大学齐鲁医学院基础医学院病原生物学系, 山东 济南 250012;2.蚌埠医学院第一附属医院检验科, 安徽 蚌埠 233004;3.山东大学齐鲁医院基础医学研究所, 山东 济南 250012;4.曼尼托巴大学药学院免疫学系, 加拿大 温尼伯R3E 0T5
  • 发布日期:2022-09-27
  • 通讯作者: 王红. E-mail:hongwang@sdu.edu.cn
  • 基金资助:
    国家自然科学基金青年科学基金(81501761);山东省重点研发计划(2017GSF18197);安徽省高校自然科学基金(KJ2018A1001);蚌埠医学院第一附属医院科技发展基金(Byyfykj201802)

Natural killer cells regulate Th17/Treg cell immune response through modulating the function of dendritic cells during chlamydial lung infection

ZHOU Xiaoqing1, LI Jing1,2, ZHAO Lei3, TANG Wei1, YANG Xi1,4, WANG Hong1   

  1. 1. Department of Pathogenic Biology, Basic Science of Medical School, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Laboratory Medicine, First Affiliated Hospital of Bengbu Medical College, Bengbu 233004, Anhui, China;
    3. Institute of Basic Medical Science, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    4. Department of Immunology, Faculty of Medicine, University of Manitoba, Winnipeg R3E 0T5, Canada
  • Published:2022-09-27

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摘要： 目的 探索鼠衣原体(Cm)肺部感染中自然杀伤(NK)细胞调节Th17/Treg应答的作用机制。 方法 建立鼠Cm肺部感染模型,采用CD11c+磁珠分选NK细胞去除鼠和同型对照抗体处理鼠的脾树突状细胞(DCs),转输至野生鼠体内,对应小鼠分为NK-DCs组(n=13)和NK+DCs组(n=13)。经Cm攻击感染后,比较两组小鼠疾病严重程度;流式细胞术分析脾和肺中Th17及Treg应答变化。 结果 与转输NK+DCs小鼠相比,转输NK-DCs的小鼠病情加重,表现为体质量下降、肺载菌量增加及病理损伤加重,同时脾和肺中Th17应答降低,Treg应答增强,Th17/Treg应答失衡,差异均有统计学意义。 结论 在Cm肺部感染中,NK细胞能够通过调控DCs有效启动Th17应答,抑制Treg应答,发挥抗感染免疫保护作用。

关键词: 衣原体, 自然杀伤细胞, 树突状细胞, Th17/Treg应答, 肺部感染

Abstract: Objective To explore the mechanism of natural killer(NK)cells in regulating of Th17/Treg cell responses during chlamydia muridarum(Cm)lung infection. Methods A pulmonary infection model of Cm was established. The spleen dendritic cells(DCs)from Cm-infected mice with or without NK cells depletion were obtained by application of CD11c+ MicroBeads. The naïve mice were given intravenous injection of DCs from different groups of mice, and divided into NK-DCs recipients group(n=13)and NK+DCs recipients group(n=13). After adoptive transfer, the same infection method was used to recipient mice with Cm. The degree of disease was compared between the two groups. Flow cytometry was used to detect the changes of Th17 and Treg cells in the spleen and lung of mice. Results Compared with NK+DCs recipients, NK-DCs recipients exhibited aggravated disease, higher weight loss and severe lung injury. Immunological analysis showed the proportion of Th17 cells in spleen and lung was reduced while the proportion of Treg cell response was enhanced, which contributed to imbalance of Th17/Treg response in NK-DCs recipients. 山 东 大 学 学 报 (医 学 版)57卷4期 -周小青,等.自然杀伤细胞通过树突状细胞调控衣原体肺部感染中Th17/Treg免疫应答平衡 \=- Conclusion NK cells can effectively initiate Th17 cells response and inhibit Treg cell response by modulating DCs and thus play an important role in controlling chlamydial infection.

Key words: Chlamydia, Natural killer cells, Dendritic cells, Th17/Treg response, Lung infection

中图分类号: 

  • R374
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