山东大学学报 (医学版) ›› 2018, Vol. 56 ›› Issue (1): 70-75.doi: 10.6040/j.issn.1671-7554.0.2017.138
• • 上一篇
丁长宽,陈健行
DING Changkuan, Jason Chen
摘要: 目的 观察蛋白酶体抑制剂硼替佐米(BTZ)联合顺铂(CIS)对宫颈癌HeLa细胞增殖的影响并研究其相关机制。 方法 将Hela细胞分为NC组、CIS组、BTZ组、C&B组,分别以DMEM培养液、含CIS的培养液、含BTZ的培养液、含CIS及BTZ的培养液培养。以CCK-8法检测各组细胞的增殖抑制率,Western blotting法检测各组细胞中p53与Rb蛋白的表达水平,流式细胞仪检测细胞凋亡率。组间比较采用析因设计资料的方差分析。 结果 C&B组细胞增殖抑制率高于其他各组(P<0.05)。CIS组及C&B组中p53表达量较高(P<0.05),BTZ组与NC组相比差异无统计学意义。与NC组相比,BTZ组Rb表达量较高(P<0.05),CIS组、C&B组与NC组相比,差异无统计学意义。流式结果示CIS和BTZ在诱导细胞凋亡方面具有交互作用。 结论 CIS可能通过调控p53通路对宫颈癌HeLa细胞增殖产生抑制作用,而BTZ可能通过调控Rb通路对宫颈癌HeLa细胞增殖产生抑制作用,且BTZ与CIS联用在抑制HeLa细胞增殖方面具有协同交互作用。
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