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山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (2): 61-67.doi: 10.6040/j.issn.1671-7554.0.2016.995

• 基础医学 • 上一篇    下一篇

柽柳对小鼠酒精性肝损伤的保护作用及机制

张钰,韩琛,王朝霞,王兆朋,张月英,周淑萍,马冉冉,王恒孝   

  1. 济南大学 山东省医学科学院医学与生命科学学院, 山东 济南 250062
  • 收稿日期:2016-08-13 出版日期:2017-02-10 发布日期:2017-02-10
  • 通讯作者: 王恒孝. E-mail:wanghengxiao@aliyun.com E-mail:wanghengxiao@aliyun.com
  • 基金资助:
    山东省科技重大专项(2015ZDJS03002)

Protective effects of Tamarix chinensis Lour on mice with alcoholic liver injury and its mechanism

ZHANG Yu, HAN Chen, WANG Zhaoxia, WANG Zhaopeng, ZHANG Yueying, ZHOU Shuping, MA Ranran, WANG Hengxiao   

  1. School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250062, Shandong, China
  • Received:2016-08-13 Online:2017-02-10 Published:2017-02-10

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摘要： 目的 观察柽柳乙醇提取物对小鼠酒精性肝损伤的保护作用,并对其分子机制进行初步探讨。 方法 采用慢性乙醇喂养加急性乙醇灌胃法诱导小鼠酒精性肝损伤。将80只雄性C57BL/6J小鼠随机分为5组:正常对照组、模型组、阳性对照组、柽柳低剂量组和柽柳高剂量组,每组16只。比较各组小鼠体质量、肝质量和肝指数之间的差别;通过HE染色和油红O染色观察肝组织的脂肪蓄积和损伤情况;检测外周血谷丙转氨酶(ALT)和甘油三酯(TG)以及肝脏中谷草转氨酶(AST)、超氧化物歧化酶(SOD)和丙二醛(MDA)水平;免疫组化法检测核苷酸结合寡聚化结构域样受体家族pyrin结构域蛋白3(NLRP3)炎性小体组成成份［NLRP3、凋亡相关微粒蛋白(ASC)、半胱氨酸蛋白酶-1(caspase-1)］和相关炎性因子IL-1β的蛋白表达水平。 结果 与模型组相比,柽柳高、低剂量组小鼠肝脏指数显著降低(P<0.05),肝脏脂肪蓄积和炎症情况明显减轻,血清中ALT和肝脏中AST水平显著降低(P<0.01);柽柳高剂量组肝脏MDA含量显著降低(P<0.05),SOD活性显著升高(P<0.05),肝脏中NLRP3、ASC、caspase-1和IL-1β的表达均显著减少(P<0.05)。 结论 柽柳可以在一定程度上抑制小鼠酒精性肝损伤的发生发展,其机制可能与抑制NLRP3-caspase-1-IL-1β通路活化有关。

关键词: 柽柳, 酒精性肝损伤, IL-1β, NLRP3炎性小体

Abstract: Objective To study the protective effects and mechanism of the ethanol extract of Tamarix chinensis Lour on mice with alcoholic liver injury. Methods Alcoholic liver injury was induced in mice through chronic plus binge alcohol feeding. Eighty male C57BL/6J mice were randomly divided into 5 groups: normal control group, model group, positive control group, Tamarix chinensis Lour with low dose group and Tamarix chinensis Lour with high dose group. The body weight, liver weight and liver index of mice were compared between these groups. The liver fat accumulation and liver injury situation were observed by HE staining and oil red O staining. Biochemical markers of hepatic damage such as serum glutamic pyruvic transaminase(ALT), serum triglyceride(TG), heptic glutamic oxaloacetic transaminase(AST), superoxide dismutase(SOD)and malondialdehyde(MDA)were determined in all groups. The protein expression levels of NOD-like receptor family, pyrin domain containing3(NLRP3), apoptosis-associated speck-like protein containing a CARD(ASC), cysteinyl aspartate specific proteinase-1(caspase-1)and proinflammatory cytokines IL-1β were determined by immunohistochemistry method. Results Compared with the model group, the liver index of mice in Tamarix chinensis Lour with low dose and high dose groups decreased significantly(P<0.05), 山 东 大 学 学 报 (医 学 版)55卷2期 -张钰,等.柽柳对小鼠酒精性肝损伤的保护作用及机制 \=-hepatic fat accumulation and inflammation alleviated significantly, and the levels of ALT in serum and AST in liver tissues were significantly decreased(P<0.01); MDA content decreased significantly(P<0.05), the activity of SOD increased significantly(P<0.05), the expressions of NLRP3, ASC, caspase-1 and IL-1β in liver was significantly reduced(P<0.05)in Tamarix chinensis Lour with low dose group. Conclusion Tamarix chinensis Lour can prevent alcohol-induced liver injury occurrence and development, whose mechanism may be related to the inhibition of NLRP3-caspase-1-IL-1β signaling.

Key words: NLRP3 Inflammasome, Alcoholic liver injury, Tamarix chinensis Lour, IL-1β

中图分类号: 

  • R967
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