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山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (9): 72-76.doi: 10.6040/j.issn.1671-7554.0.2014.314

• 临床医学 • 上一篇    下一篇

自噬相关蛋白4EBP1与eIF4E在几种常见脑瘤组织中的表达

许加军1,2, 刘宗现1, 许风雷3, 王磊1, 孔维华1   

  1. 1. 山东大学生命科学学院, 山东 济南 250100;
    2. 山东大学附属省立医院神经外科, 山东 济南 250021;
    3. 山东大学齐鲁医院耳鼻咽喉科, 山东 济南 250012
  • 收稿日期:2014-05-15 修回日期:2014-09-06 出版日期:2014-09-10 发布日期:2014-09-10
  • 基金资助:
    山东省自然科学基金重点项目(ZR2010CZ001)；山东省优秀中青年科学家科研奖励基金(BS200403017)；山东大学自主创新基金(2012JC022)

Expression of autophagy pathway proteins eIF4E and 4EBP1 in several cerebroma tissues

XU Jiajun1,2, LIU Zongxian1, XU Fenglei3, WANG Lei1, KONG Weihua1   

  1. 1. School of Life Science, Shandong University, Jinan 250100, Shandong, China;
    2. Department of Neurosurgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    3. Department of Otolaryngology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-05-15 Revised:2014-09-06 Online:2014-09-10 Published:2014-09-10
  • Contact: 孔维华。E-mail:whkong@sdu.edu.cn E-mail:whkong@sdu.edu.cn

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摘要： 目的 探讨自噬相关蛋白4EBP1与eIF4E在星形细胞瘤、脑膜瘤、少突胶质细胞瘤、胆脂瘤和正常脑组织中的表达及其差异。方法 应用逆转录聚合酶链式反应（RT-PCR）法检测4种脑瘤中4EBP1和eIF4E mRNA的表达情况，采用免疫组化SABC法和Western blotting检测4EBP1和eIF4E在4种脑瘤组织中的表达情况，并应用SPSS 17.0统计软件，分析4EBP1和eIF4E在4种脑瘤以及正常脑组织中的表达差异。结果 4种脑瘤细胞均转录4EBP1和eIF4E mRNA，表达4EBP1、eIF4E蛋白，同时伴随着自噬标志蛋白LC-3的表达和LAMP-2A的高表达。免疫组化结果显示，与正常组织相比，4EBP1和eIF4E在4种肿瘤中的表达量明显升高，方差分析结果表明4EBP1和eIF4E在4种肿瘤组织与正常脑组织中的表达差异有统计学意义（P<0.01）。结论 自噬相关蛋白4EBP1和eIF4E在4种脑肿瘤组织中的表达明显上调，说明4EBP1和eIF4E及其自噬信号通路在肿瘤发生、发展中可能发挥重要作用，可作为脑瘤治疗的潜在靶标以及为临床诊断提供一种新的依据。

关键词: 真核起始因子结合蛋白1, 真核起始因子, 脑瘤, 免疫组化

Abstract: Objective To explore the expressions of 4EBP1 and eIF4E in astrocytoma, meningeoma, oligdendrocytoma, cholesteatoma and normal brain tissues and their differences. Methods Reverse transcriptase polymerase chain reaction (RT-PCR) was adopted to determine the levels of 4EBP1 and eIF4E mRNAs in four cerebromas. Both immunohistochemistry and Western blotting methods were applied to detect the expressions of eIF4E and 4EBP1 proteins in four cerebromas. SPSS 17.0 was used to analyze the differences of expressions of 4EBP1 and eIF4E in four cerebromas as well as normal tissues. Results The mRNAs and proteins of eIF4E and 4EBP1 were observed in four cerebromas. Meanwhile, the proteins of LC-3 and LAMP-2A increased significantly. The results of immunohistochemistry suggested that the levels of eIF4E and 4EBP1 were higher in meningeoma, cholesteatoma, astrocytoma and oligdendrocytoma compared with those in normal tissues. The results of analysis of variance showed that in comparison to normal brain tissues, the expressions of eIF4E and 4EBP1 in astrocytoma, meningeoma, oligdendrocytoma, and cholesteatoma hadstatistically significant (P<0.01). Conclusion The expressions of eIF4E and 4EBP1 are obviously up-regulated in four cerebromas, which suggests that eIF4E, 4EBP1 and their autophagy signaling pathways play an important role in the development of cancer; therefore, eIF4E and 4EBP1 can act as potential therapeutic targets as well as evidence for clinical diagnosis.

Key words: Eukaryotic translation initiation factor 4E, Eukaryotic translation initiation factor 4E-binding protein 1, Cerebroma, Immunohistochemistry

中图分类号: 

  • R739.4
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