西洋参茎叶皂苷对脑梗死大鼠ET-1、VEGF及脑梗死面积的影响

doi:10.6040/j.issn.1671-7554.0.2014.166

山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (9): 30-33.doi: 10.6040/j.issn.1671-7554.0.2014.166

西洋参茎叶皂苷对脑梗死大鼠ET-1、VEGF及脑梗死面积的影响

付庆元¹, 戴桂强¹, 张松², 安洪春³, 梁世民¹

1. 济南市第五人民医院急诊科, 山东济南 250022;
2. 山东大学齐鲁医院神经内科, 山东济南 250012;
3. 济南市第一人民医院神经内科, 山东济南 250011

收稿日期:2014-03-23 修回日期:2014-08-29 出版日期:2014-09-10 发布日期:2014-09-10
基金资助:
济南市科技局项目(201221026)

Effect of panax quinquefolium saponin from stems and leaves on endothelin-1, vascular endothelial growth factor and infarct area in rats

FU Qingyuan¹, DAI Guiqiang¹, ZHANG Song², AN Hongchun³, LIANG Shimin¹

1. Department of Emergency, the 5th People's Hospital of Jinan, Jinan 250022, Shandong, China;
2. Department of Neurology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
3. Department of Neurology, the 1th People's Hospital of Jinan, Jinan 250011, Shandong, China

Received:2014-03-23 Revised:2014-08-29 Online:2014-09-10 Published:2014-09-10
Contact: 付庆元。E-mail:fqy132@163.com E-mail:fqy132@163.com

摘要/Abstract

摘要： 目的探讨西洋参茎叶皂苷（PQS）对脑梗死大鼠内皮素-1（ET-1）、血管内皮细胞生长因子（VEGF）及梗死面积的影响。方法采用栓线法对大鼠右侧大脑中动脉进行栓塞并随机分成假手术组、模型对照组、尼莫地平组、PQS高、低剂量组，用药后检测各组神经功能缺损情况、大鼠脑组织中ET-1及VEGF含量、大鼠脑梗死面积，并对各组检测数据进行比较分析。结果 PQS高、低剂量组神经功能损伤恢复状况均比尼莫地平组效果明显（P<0.05）；与模型对照组比较，尼莫地平组、PQS低、高剂量组、假手术组脑组织中ET-1含量呈现明显下降趋势（P<0.05），各组之间两两比较差异均有统计学意义（P<0.05）；PQS高剂量组效果最好；PQS高剂量组VEGF含量均高于其余各组（P<0.05）；PQS高剂量组脑梗死面积最小，效果明显（P<0.05），PQS随着剂量的增加，脑缺血区有明显好转，可见少量胶质细胞增生。结论 PQS对提高血管活性、改善脑梗死病情具有显著效果。

关键词: 西洋参茎叶皂苷, 血管内皮细胞生长因子, 内皮素-1, 梗死面积

Abstract: Objective To explore the effect of panax quinquefolium saponin (PQS) from stems and leaves on endothelin-1 (ET-1), vascular endothelial growth factor (VEGF) and cerebral infarct area in rats. Methods Rats were randomly divided into sham operation group, model control group, nimodipine group, high doses of PQS group and low doses of PQS group, after the right cerebral arteries were bolted to induce embolization. The neurological deficit, ET-1 and VEGF levels, and infarct area in each group were detected and compared. Results The neurological function recovered better in high and low doses of PQS groups than in other groups (P<0.05). Compared with the model control group, the other groups had significantly declined ET-1 expressions (P<0.05), and there was statistical difference between any two groups (P<0.05). The high doses of PQS group had the highest VEGF expression but the least infarct area compared with the other groups. Conclusion Panax quinquefolium saponin from stems and leaves has a significant effect to enhance vascular activity and thus relieve cerebral infarction.

Key words: Endothelin-1, Panax quinquefolium saponin from steams and leaves, Vascular endothelial growth factor, Infarct area

中图分类号:

R743.32

付庆元, 戴桂强, 张松, 安洪春, 梁世民. 西洋参茎叶皂苷对脑梗死大鼠ET-1、VEGF及脑梗死面积的影响[J]. 山东大学学报（医学版）, 2014, 52(9): 30-33.

FU Qingyuan, DAI Guiqiang, ZHANG Song, AN Hongchun, LIANG Shimin. Effect of panax quinquefolium saponin from stems and leaves on endothelin-1, vascular endothelial growth factor and infarct area in rats[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(9): 30-33.

参考文献

[1] Fu H Y, Okada K, Liao Y, et al. Ablation of C/EBP homologous protein atteuates endoplasmic reticulum-mediated apoptosis and cardiac dysfunction induced by pressure overload[J]. Circulation, 2010, 122(4): 361-369.
[2] 郭春雨,刘倩,石颖,等. 西洋参茎叶总皂苷对心肌梗死大鼠非梗死区组织的保护作用[J]. 中华老年心脑血管病杂志,2012,14(7):748-751. GUO Chunyu, LIU Qian, SHI Ying, et al. Protective effect of ginseng saponin on myocardial infarction in rats non-infarcted tissue[J]. Chinese Journal of Cardiovascular and cerebrovascular Diseases in elderly, 2012, 14(7):748-751.
[3] 刘哲,张峰,王平,等. 西洋参皂苷对新生鼠缺血-再灌注心肌细胞凋亡的影响及机制[J]. 中国兽医杂志,2010, 46(7)∶23- 25. LIU Zhe, ZHANG Feng, WANG Ping, et al. Effects and mechanisms of ginseng saponins on newborn rats ischemia-reperfusion myocardial cell apoptosis[J].Chinese Journal of Veterinary Medicine, 2010, 46( 7):23-25.
[4] 王怀刚,关利新,赵鑫,等. 西洋参茎叶皂苷对局灶性脑缺血后大鼠血清中GFAP表达的影响[J].中医药学报,2011,39(4):17-19. WANG Huaigang, GUAN Lixin, ZHAO Xin, et al. Impact of ginseng stems and leaves saponins on serum GFAP expression in rats after focal cerebral ischemia[J]. Traditional Chinese Medicine, 2011, 39(4):17-19.
[5] 甘照儒.中药对缺血性脑损伤保护作用的分子机制研究进展[J].中西医结合心脑血管病杂志, 2009, 7(7):829-831. GAN Zhaoru.Advances of molecular mechanisms on ischemic brain injury in traditional medicine[J]. Journal of Integrative Medicine About Cardiovascular Disease, 2009, 7(7):829-831.
[6] Chitnis N S, Pytel D, Bobrovnikova-Maijon E, et al. miR-211 is a prosurvival microRNA that regulates chop expression in a PERK-dependent manner[J]. Mol Cell, 2012, 48(9): 353-364.
[7] Xin W, Li X, Lu X, et al. Involvement of endoplasmic reticulum stress-associated apoptosis in a heart failure model induced by chronic myocardial ischemia[J]. Int J Mol Med, 2011, 27(4): 503-509.
[8] Chipuk J E, Moldoveanu T, Llambi F, et al. The BCL-2 family reunion[J]. Mol Cell, 2010, 37(3):299- 310.
[9] 张颖,陈可冀, 杨领海,等. 西洋参茎叶总皂苷对脂肪细胞糖脂代谢及胰岛素抵抗信号转导的影响[J]. 中国中西医结合杂志,2010,7(3):231-235. ZHANG Ying, CHEN Keji, YANG Linghai, et al. Impact of ginseng stems and leaves saponins on glucose and lipid metabolism and insulin resistance signal transduction[J]. Chinese Journal of Integrative Medicine, 2010, 7(3):231-235.
[10] 尹艳艳,李卫平,李维祖,等.黄芪总苷对大鼠局灶性脑缺血/再灌注损伤后BDNF、TrkB和p75NTRmRNA表达的影响[J].中国药理学通报,2009,25(5):672-676. YIN Yanyan, LI Weiping, LI Weizu, et al. Effects of Astragalosides on BDNF, TrkB and p75NTRmRNA expression in rats after focal cerebral ischemia / reperfusion injury[J]. Chinese Pharmacological Bulletin, 2009, 25(5):672-676.
[11] 许爱梅, 庄向华, 董砚虎, 等.内皮素的研究进展[J] .国外医学内科学分册, 2004, 31(5): 198-200. XU Aimei, ZHUANG Xianghua, DONG Yanhu, et al. Progressive Research of endothelin[J].Volume in the International Journal of Medicine, 2004, 31(5): 198-200.
[12] 张伟三, 张蔷, 张蕴, 等. 慢性间歇低氧老龄大鼠脑血管内皮功能的研究[ J]. 中华老年医学杂志, 2010, 29(7): 601. ZHANG Weisan, ZHANG Qiang, ZHANG Yun, et al. Research of vascular endothelial function in aged rats with chronic intermittent hypoxia brain[J]. Chinese Journal of Geriatrics, 2010, 29(7): 601.
[13] 王琛,刘密,王晓锜,等. 西洋参茎叶总皂苷减轻大鼠心肌细胞缺氧/复氧损伤机制的研究[J].中国病理生理杂志,2013,29(10):1798-1802. WANG Chen, LIU Mi, WANG Xiaoqi, et al. Mechanism of ginseng stems and leaves saponins on rat myocardial cells during hypoxia / reoxygenation injury[J]. Chinese Journal of Pathophysiology, 2013, 29(10):1798-1802.
[14] Suchting S, Freitas C, le Noble F, et al. The Notch ligand Delta-like negatively regulates endothelial tip cell formation and vessel branching[J]. Proc Natl Acad Sci USA, 2007, 104(9):3225-3230.
[15] 李增宁,关利新,魏新刚,等. 西洋参茎叶皂苷对局灶性脑缺血后caspase-9表达的影响[J]. 成都医学院学报,2010,5(2):110-113. LI Zengning, GUAN Lixin, WEI Xingang, et al. Impact of ginseng stems and leaves saponins on expression of caspase-9 after focal cerebral ischemia[J]. Journal of Chengdu Medical College, 2010, 5(2):110-113.

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西洋参茎叶皂苷对脑梗死大鼠ET-1、VEGF及脑梗死面积的影响

Effect of panax quinquefolium saponin from stems and leaves on endothelin-1, vascular endothelial growth factor and infarct area in rats

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