您的位置：山东大学 -> 科技期刊社 -> 《山东大学学报（医学版）》

山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (7): 28-31.doi: 10.6040/j.issn.1671-7554.0.2013.775

• 基础医学 • 上一篇    下一篇

前扣带皮层吻侧部注射NR2B受体阻断剂Ifenprodil对骨癌痛大鼠伴随痛厌恶情绪体验的影响

邹旭丽1, 宫本航1, 张孟元1, 冯颢2, 王公明1   

  1. 1. 山东大学附属省立医院麻醉科, 山东 济南 250021;
    2. 山东大学第二医院麻醉科, 山东 济南 250033
  • 收稿日期:2013-12-30 修回日期:2014-06-12 出版日期:2014-07-10 发布日期:2014-07-10
  • 通讯作者: 张孟元。E-mail：zhangmy717@163.com；王公明。E-mail：tagmwang1971@163.com E-mail:zhangmy717@163.com;tagmwang1971@163.com
  • 基金资助:
    国家自然科学基金（30872433）；山东省科技攻关基金（2010GW2023P）

Effect of Ifenprodil administered into the rostral anterior cingulate cortex on the pain-related aversion in rats with bone-cancer pain

ZOU Xuli1, GONG Benhang1, ZHANG Mengyuan1, FENG Hao2, WANG Gongming1   

  1. 1. Department of Anesthesiology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    2. Department of Anesthesiology, the Second Hospital of Shandong University, Jinan 250033, Shandong, China
  • Received:2013-12-30 Revised:2014-06-12 Online:2014-07-10 Published:2014-07-10

PDF (PC)

149

摘要： 目的 探讨前扣带皮层吻侧部（rACC）内注射N-甲基-D-天门冬氨酸受体2B亚基（NR2B）受体阻断剂Ifenprodil是否减轻骨癌痛大鼠伴随的痛厌恶情绪体验。方法 无位置偏爱的雄性Wistar大鼠30只随机分为空白对照组、骨癌痛组和Ifenprodil组，每组10只。骨癌痛组和Ifenprodil组大鼠右胫骨骨髓腔接种MADB-106细胞3 μL（4.8×109 /mL），而空白对照组给予同等剂量的生理盐水作对照。术后第14天 Ifenprodil组rACC内给予NR2B受体阻断剂Ifenprodil 1.2 μL，而空白对照组和骨癌痛组给予同等剂量的生理盐水。分别于术前1天，以及术后第3、7、10、12及14天采用Von Frey刺激法测量大鼠右后爪机械刺激痛阈的变化。术后第14天rACC内给予Ifenprodil后观测骨癌痛大鼠伴随的痛厌恶情绪体验。结果 骨癌痛组及Ifenprodil组大鼠自术后第10天至第14天右后爪机械刺激痛阈值明显降低（P<0.05），而空白对照组无改变（P>0.05）。组间比较术后第10、12和14天骨癌痛组和Ifenprodil组明显低于空白对照组（P<0.05）。条件性位置回避实验显示，骨癌痛组大鼠A室滞留时间的百分比为（30±4）%，低于空白对照组（52±5）%（P<0.05）；经rACC内注射Ifenprodil后A室滞留时间的百分比为（42±5）%，较骨癌痛组升高但仍低于空白对照组（P<0.05）。结论 前扣带回皮层注射NR2B受体阻断剂ifenprodil能够减轻骨癌痛大鼠伴随的痛厌恶情绪体验。

关键词: Ifenprodil, 条件性位置回避, 前扣带皮层吻侧, 骨癌痛, 厌恶情绪体验

Abstract: Objective To investigate the effect of intra-rACC (rostral anterior cingulate cortex, rACC) administration of Ifenprodil on aversion related to bone-cancer pain. Methods Totally 30 male Wistar rats without place preference were randomly divided into three groups:control group (n=10), bone-cancer pain group (n=10) and Ifenprodil group (n=10). A total of 3 μL MADB-106 cells (4.8×109/mL) were inoculated into the right tibia bone marrow cavity in rats of the bone-cancer pain group and Ifenprodil group; rats of the control group were given the same dose of normal saline (NS). A total volume of 1.2 μL Ifenprodil was administered into the rACC on the 14th day after operation, while the control group and bone-cancer pain group were given the same dose of NS. The diversity of the mechanical stimulation withdrawal threshold was measured using Von Frey stimulation on the 1st day before the operation, and the 3rd, 7th, 10th, 12th and 14th day after the operation. On the 14th day after injection of Ifenprodil, the pain-related aversion in rats was observed. Results After the operation, the mechanical stimulation withdrawal threshold decreased obviously inrats of the bone-cancer pain group and Ifenprodil group from the 10th day to the 14th day (P<0.05). Group comparison showed that the pain threshold to mechanical stimulation of rats in the bone-cancer pain group and Ifenprodil group decreased significantly compared with that of the control group by day 10th, 12th and 14th after inoculation with the tumor cells (P<0.05). The residence time within chamber A was (30±4)% in bone-cancer pain group, lower than that of the (52±5)% in the control group (P<0.05). After Ifenprodil treatment, the residence time within chamber A of the Ifenprodil group was (42±5)%, higher than that of the bone-cancer pain group, but still lower than that of the control group (P<0.05). Conclusion Ifenprodil, a selective NR2B antagonist, administered into the rACC, can effectively alleviate pain-related aversion in rats with tibial bone cancer.

Key words: Conditioning place avoidance, Pain-related aversion, Ifenprodil, Bone-cancer pain, Rostral anterior cingulate cortex

中图分类号: 

  • R453
[1] Quartana P J, Campbell C M, Edwards R R. Pain catastrophizing:a critical review[J]. Expert Rev Neurother, 2009, 9(5):745-758.
[2] Kumar S P. Cancer Pain:a critical review of mechanism-based classification and physical therapy management in palliative care[J]. Indian J Palliat Care, 2011, 17(2):116-126.
[3] Zhong X L, Wei R, Zhou P, et al. Activation of anterior cingulated cortex extracellular signal-regulated kinase-1 and -2 (ERK1/2) regulates acetic acid-induced, pain-related anxiety in adult female mice[J]. Acta Histochem Cytochem, 2012, 45(4):219-225.
[4] Qu C, King T, Okun A, et al. Lesion of the rostral anterior cingulate cortex eliminates the aversiveness of spontaneous neuropathic pain following partial or complete axotomy[J]. Pain, 2011, 152(7):1641-1648.
[5] Lei L G, Sun S, Gao Y J, et al. NMDA receptors in the anterior cingulate cortex mediate pain-related aversion[J]. Exp Neurol, 2004, 189(2):413-421.
[6] Johansen J P, Fields H L. Glutamatergic activation of anterior cingulate cortex produces an aversive teaching signal[J]. Nat Neurosci, 2004, 7(4):398-403.
[7] Medhurst S J, Walker K, Bowes M, et al. A rat model of bone cancer pain[J]. Pain, 2002, 96(1-2):129-140.
[8] Paxinos G, Watson C. The rat brain in stereotaxic coordinates[M]. San Diego. Academic Press, 1982: 5-100.
[9] Bravo L, Mico J A, Rey-Brea R, et al. Depressive-like states the aversion to painful stimuli in a rat model of comorbid chronic and depression[J]. Anesthesiology, 2012, 117(3):613-625.
[10] 张玉秋.痛情绪和相关记忆产生的神经机制[J].自然科学进展, 2005, 15(12):1409-1415.
[11] 赵倩倩, 杜其航, 王守靓, 等. 胫骨癌痛大鼠痛觉敏化的形成及其脊髓机制[J]. 山东大学学报:医学版, 2012, 50(7):37-40.
[1] 赵倩倩，杜其航，王守靓，王公明，张孟元. 胫骨癌痛大鼠痛觉敏化的形成及其脊髓机制[J]. 山东大学学报(医学版), 2012, 50(7): 37-.
Viewed
Full text


Abstract

Cited
  Shared   
  Discussed   
No Suggested Reading articles found!
版权所有 © 2018 《山东大学学报(医学版)》编辑部　
地址:山东大学科技期刊社（济南市历城区山大南路27号山东大学中心校区明德楼B座721室） 电话: 0531-88366918 E-mail: xbyxb@sdu.edu.cn
本系统由北京玛格泰克科技发展有限公司设计开发