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山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (7): 32-36.doi: 10.6040/j.issn.1671-7554.0.2013.702

• 基础医学 • 上一篇    下一篇

负载凋亡肿瘤细胞的树突状细胞对喉癌细胞的杀伤作用

张敏1, 王雪峰2   

  1. 1. 辽宁医学院, 辽宁 锦州 121001;
    2. 辽宁医学院附属第一医院耳鼻咽喉头颈外科, 辽宁 锦州 121001
  • 收稿日期:2013-11-23 修回日期:2014-06-10 出版日期:2014-07-10 发布日期:2014-07-10
  • 通讯作者: 王雪峰。E-mail：jzswwang@126.com E-mail:jzswwang@126.com
  • 基金资助:
    辽宁省科学技术计划项目（2012225019）

Killing effect of apoptotic-tumor-cell-loaded dendritic cells on laryngeal carcinoma cells

ZHANG Min1, WANG Xuefeng2   

  1. 1. Liaoning Medical University, Jinzhou 121001, Liaoning, China;
    2. Department of Otolaryngology Head and Neck Surgery, the First Affiliated Hospital of Liaoning Medical University, Jinzhou 121001, Liaoning, China
  • Received:2013-11-23 Revised:2014-06-10 Online:2014-07-10 Published:2014-07-10

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摘要： 目的 探讨凋亡肿瘤细胞抗原负载的树突状细胞（DC）对喉癌细胞杀伤作用的影响。方法 用细胞因子重组人白细胞介素4（rhIL-4）、重组人粒细胞巨噬细胞集落刺激因子（rhGM-CSF）与肿瘤坏死因子α（rhTNF-α）体外诱导扩增人外周血单个核来源的DC，6 d后将DC分为凋亡组、冻融组和未处理组；各组细胞继续培养4 h后，分别采用流式细胞术检测DC表面分子的表达、ELISA法检测干扰素γ（IFN-γ）的水平、MTT法评估各组DC刺激细胞毒性T淋巴细胞（CTL）增殖的能力及诱导CTL的杀伤活性。结果 与其余各组相比，凋亡组DC表面分子与IFN-γ的表达明显增加（P<0.05），CTL的增殖能力与杀伤活性增强（P<0.05）。结论 凋亡肿瘤细胞可以促进DC的分化与成熟，并能使DC产生高效而特异性的抗喉癌免疫应答。

关键词: 树突状细胞, 凋亡肿瘤细胞, 喉癌细胞, 顺铂

Abstract: Objective To explore the killing effect of apoptotic-tumor-cell-loaded dendritic cells (DCs) on the laryngeal carcinoma cells. Methods DCs from peripheral blood monocytes were induced by recombinant human interleukin-4 (rhIL-4), recombinant human granulocyte/macrophage colony-stimulating factor (rhGM-CSF) and tumor necrosis factor (TNF-α) in vitro, then were divided into the apoptotic laryngeal carcinoma cells group, freeze-thaw cells group and untreated cells group 6 days later. After continuous culture of DCs for 4h, the expressions of the surface markers of DCs were detected by flow cytometry, the level of Interferon-γ (IFN-γ) was measured by ELISA, and the proliferation ability and killing effect of cytotoxic T lymphocyte (CTL) stimulated by DCs were evaluated by MTT. Results Compared with other groups, apoptotic laryngeal carcinoma cells group could enhance the expression of the surface markers CD83, CD86, HLA-DR and IFN-γ(P<0.05), and the proliferation ability and killing effect of CTL increased (P<0.05). Conclusion Apoptotic carcinoma cells can promote the differentiation and maturation of DCs, and induce effective and specific immune response against laryngeal carcinoma.

Key words: Dendritic cell, Laryngeal carcinoma cell, Cisplatin, Apoptotic carcinoma cells

中图分类号: 

  • R730.51
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