白藜芦醇体外抗肠球菌作用的超微结构变化

doi:10.6040/j.issn.1671-7554.0.2013.234

山东大学学报（医学版） ›› 2014, Vol. 52 ›› Issue (2): 16-19.doi: 10.6040/j.issn.1671-7554.0.2013.234

白藜芦醇体外抗肠球菌作用的超微结构变化

苏军华1，时东彦1，王磊2，李伟皓1，杜素苹3，李永军1

1.河北医科大学第二医院检验科，石家庄 050000； 2.河北省保定市高阳县医院检验科，河北高阳 071500；
3.河北医科大学第二医院妇产科，石家庄 050000

收稿日期:2013-04-01 出版日期:2014-02-10 发布日期:2014-02-10
通讯作者: 李永军， E-mail:lyjj221@sina.com
基金资助:
河北省科技支撑项目(12277711)

In vitro antibacterial activity of resveratrol against Enterococcus

SU Jun-hua1, SHI Dong-yan1, WANG Lei2, LI Wei-hao1, DU Su-ping3, LI Yong-jun1

1. Department of Clinical Laboratory, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China;
2. Department of Clinical Laboratory, Gaoyang County Hospital of Hebei Province, Gaoyang 071500, Hebei, China;
3. Department of Gynecology and Obstetrics, the Second Hospital of Hebei Medical University, Shijiazhuang 050000, China

Received:2013-04-01 Online:2014-02-10 Published:2014-02-10

摘要/Abstract

摘要：

目的观察白藜芦醇(RES)对肠球菌体外抑制作用的超微结构变化，进一步探讨其作用机制。方法根据美国临床实验室标准化委员会标准，使用微量肉汤稀释法对RES进行定量抗菌实验，获得白藜芦醇对粪肠球菌和屎肠球菌的最低抑菌浓度(MIC)，应用扫描电镜和透射电镜观察RES对肠球菌细胞作用后微观结构的变化。结果 RES对粪肠球菌的MIC50和MIC90分别为0.256mg/mL、0.512mg/mL，对屎肠球菌的MIC50和MIC90分别为0.256mg/mL、0.512mg/mL；扫描电镜显示肠球菌细胞壁出现皱褶、破裂；透射电镜显示肠球菌细胞壁层次不清，细胞器结构模糊，细胞质内含物大多消失，出现大量空泡。结论 RES通过破坏肠球菌菌体结构而发挥抑菌作用，为进一步阐明RES的抗菌作用机制提供理论依据。

关键词: 肠球菌, 最低抑菌浓度, 扫描电镜, 透射电镜, 白藜芦醇

Abstract:

Objective To investigate the antibacterial activity and ultrastructure changes of resveratrol (RES) against Enterococcus in vitro. Methods Minimum inhibitory concentration (MIC) of RES against Enterococcus was tested with microdilution method according to the Guidelines of Clinical and Laboratory Standards Institute. Ultrastructure changes of Enterococcus against RES were observed with both scanning and transmission electron microscope. Results MIC50 and MIC90 of RES against Enterococcus faecalis were 0.256mg/mL and 0.512mg/mL, and MIC50 and MIC90 of RES against Enterococcus faecom were 0.256mg/mL and 0.512mg/mL. Shrinkage and rupture of cell wall of Enterococcus was found with scanning and transmission electron microscope when cultivated with RES. Unclear structure of cell wall and organelles, and a large quantity of vacuoles in Enterococcus were observed with transmission electron microscope. Conclusion The antibacterial activity of RES is confirmed by destroying the structure of Enterococcus.

Key words: Enterococcus, Scanning electron microscope, Resveratrol, Minimal inhibitory concentration, Transmission electron microscope

中图分类号:

R378.1

苏军华1，时东彦1，王磊2，李伟皓1，杜素苹3，李永军1. 白藜芦醇体外抗肠球菌作用的超微结构变化[J]. 山东大学学报（医学版）, 2014, 52(2): 16-19.

SU Jun-hua1, SHI Dong-yan1, WANG Lei2, LI Wei-hao1, DU Su-ping3, LI Yong-jun1. In vitro antibacterial activity of resveratrol against Enterococcus[J]. JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES), 2014, 52(2): 16-19.

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白藜芦醇体外抗肠球菌作用的超微结构变化

In vitro antibacterial activity of resveratrol against Enterococcus

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