Table of Content

  

25 June 2006
Volume 44 Issue 6

Articles


Articles

〖KH+9.2mmD]Cloning of human interleukin26 and constructing ofits eukaryotic expression vector

LIU Yiqing,WANG Lai-cheng,JIAO Yu-lian,ZHANG Jie,MA Chunyan,CUI Bin,GAO Xinpu,LIU Zheng-in,HANG Xue,ZHAO Yue-ran,

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(6):  541-544. 

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To clone the gene of human interleukin26（hIL26）and to construct its eukaryotic expression vector. Methods: Total RNA was extracted from the human peripheral blood monocytes，and reverse transcriptasepolymerase chain reaction（RTPCR）was performed. The PCR product was inserted into cloning vector pMD18T to construct the recombinant plasmid of hIL26pMD18T/hIL26. Recombinants were transformed into E.coliDH5α and screened with colony PCR and restriction analysis. The gene of hIL26 cloned was sequenced. The hIL26 gene fragment obtained from pMD18T/hIL26 was digested with SalⅠ and EcoRⅠ, and then inserted into pIRES2EGFP that was cut with XhoⅠ and EcoRⅠ. Recombinants were identified by colony PCR and restriction analysis. The recombinant expression plasmid pIRES2EGFPhIL26 was

Construction of pEGFPKallikrein expression vectorand its expression in endothelial progenitor cells in rabbit

JIANG Hong,LI Da-qing.ZHANG Yun,LIU Chun-xi,FENG Jin-bo,WANG Rong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(6):  576-579. 

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To construct pEGFPKallikrein eukaryotic expression vector by using green fluorescence protein as reportor gene and transfecting rabbit endothelial progenitor cells. Methods： The human tissue kallikrein（tHK） genes were amplified with PCR technique and inserted into pEGFPN3 vector. The rabbit endothelial progenitor cells (EPCs) were transfected with the formed plasmid by means of lipidosome. The KallikreinEGFP fused protein was viewed directly with fluorensce microscope, and the expression of tHK was detected by RTPCR(reverse transcriptionPCR) and ELISA. Results： Plasmids was formed correctly and was detected by PCR meanwhile it being sequenced and expressed in the rabbit EPCs. The fused protein has activites of both kallikrein and EGFP. Conclusion: tHK gene modified EPCs could play a role in the treatment of artery injury and endothelial dysfunction.

Establishment of the postinfectious bowel dysfunction model

GUO Min,LI Yan-qing,YU Xiu-juan,KONG Qing-lian,GUO Yu-ting,ZUO Xiu-li,ZENG Juan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(6):  586-589. 

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To establish the model of bowel dysfunction after acute shigella flexneri infection by imitating the natural course of human postinfectious irritable bowel syndrome. Methods: Shigella flexneri 1 ml in a concentration of 9×108CFU/ml was injected into 30 female Wistar rats by gavage to make models of postinfectious bowel dysfunction, and saline into the other 30 by gavage. Stool form and histological changes of distal colon, cultivated shigella flexneri were observed at day 1, 4, 7, 10, 13, and the perceptual threshold was detected with colonrectal balloon distention at day 16, 19, 22, 25 after infection. Results: At day 16～22 after acute enteric infection, stool form altered and the perceptual threshold decreased greatly in experiment group compared with normal control group (P＜0.05), while no histological inflammation was found in distal colon and no shigella flexneri growth in cultivation of feces in experiment group. Conclusion： Visceral hypersensitivity exists in rats at day 16～22 after acute shigella flexneri infection when enteral infection diminishes, suggesting postinfectious bowel dysfunction model is established successfully.

Clinical significance of expression of B7H4 in colon and rectal cancer tissues

SUN Yu-ping,LIU Jie,CUI Xia,ZHAO Dong,LIU Hui-ping,WANG Yun-shan

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(6):  590-592. 

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To investigate the role of B7H4 in the development of colon and rectal cancer(CRC). Method: The expression of B7H4 in 40 excised colon and rectal tumors was evaluated immunohistochemiscally. Results: B7H4 was high expressed (80.0%)in colon and rectal cancer. No relationship was found between the expression of B7H4 and sex, age, histology type, pathology stage, cell differentiation, or lympha metastasis. However, significantly fewer TILs was identified in B7H4positive tumor specimens than in B7H4negative tumor specimens. Conclusion: ①B7H4 may be a new marker of diagnosis for colon and rectal cancer;② These results suggest that the expression of B7H4 on tumor tissue may contribute to negative regulatory immunue responses against TILs in colon and rectal cancer. The blockade of B7H4 in colon and rectal cancer might be one strategy to pursue for future immunotherapy in colon and rectal cancer.

Role of aminoguanidine in protecting the kidney function of streptozocininduced diabetic rats

L Xue-ai,GUAN Guang-ju,WEN Rong-zhu,SUN Yun,TU Xiao-wen

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(6):  597-601. 

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To explore the alteration of protein kinase C(PKC) activity and the effect of aminoguanidine (AG) on diabetic glomerulopathy in streptozocin (STZ)induced diabetic rats. Methods： A total of 60 male Wistar rats were randomized into normal control, diabetes control and AG treatment group. Blood glucose, HbA1c，serum creatinine（Cr）, urinary albumin, creatinine clearance rate (Ccr), PKC activity and DAG level of glomerular were measured 8 and 24 weeks after the treatment and compared the results from other groups. Results： The level of blood glucose, HbA1c，urinary albumin, the PKC activity, the Ccr and the DAG level of glomerular were all significantly increased in diabetic rats. AG had no effect on the alteration of blood glucose, HbA1c and weight in diabetic rats, but decreased the level of urinary albumin, the PKC activity, the Ccr and the DAG level of glomerular. Conclusion： The rise of PKC activity plays an important role in the development of diabetic nephropathy. AG could reduce the PKC activity and may be a novel inhibitor of protein kinase C.

Predictive value of flow cytometry crossmatch to oneyear graft loss following kidney transplantation

WEN Rong-zhu,Paul Keown,Vivian Wu,LIU Gang,L XUE-ai,SONG Chun-yan,GUAN Guang-ju

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(6):  602-605. 

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Objective: Flow cytometry crossmatch (FCXM) has been extensively used in kidney transplantation, but there is no agreement regarding its predictive value for oneyear graft loss following kidney transplantation. The aim of the study was to explore the relationship between FCXM and oneyear graft loss following kidney backward stepwise method. Results: Graft loss occurred in 30 subjects during the followup, and 23 graft losses happened in the first year following kidney transplantation. χ2 test showed that there was no statistical difference regarding oneyear graft loss between FCXM positive and negative groups. Logistic regression showed that the significant predictors for oneyear graft loss were delayed graft function (OR=8.00, P=0.001?4), cadaver donor (OR=9.30, P=0.001?7) and vascular rejection (OR=5.05, P=0.021?9). FCXM was not a significant predictor with an odds ratio of 1.60 (P=0.534?6). Conclusion: FCXM has no predictive value for oneyear graft loss following kidney transplantation.transplantation. Methods: χ2 test was used to compare oneyear graft loss rate between FCXM positive and negative groups. Logistic model was built to measure the association between flow cytometry crossmatch and oneyear graft loss, and model variables included early acute rejection, vascular rejection, delayed graft function, positive FCXM, panel reactive antibody, HLAA, B mismatch number, HLADR mismatch number, donor resource, transplantation history, cold ischemic time, donor and recipient age and immunosuppression medication. The final model was determined by th

Relationship between pregnancy induced diabetes and adipocytokins

LIU Zeng-juan,PEI Feng-min,LI Yan,DING Jin-yun,ZHANG Feng

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(6):  606-609. 

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o explore the relationship between the serum adipocytokins of adiponectin, TNFα and leptinin and pregnancy induced diabetes. Methods:Radioimmunoassay was used to detect the serum adiponectin, leptin and cpeptide ELISA was applied to detect TNFα levels in 50 women with pregnancy induced diabetes and 36 normal pregnant women, electrochemiluminescent immunoassays and pressure liquid chromatography(LPLC) were used to measure serum insulin and HbA1c respectively, and glucose oxidase method to measure the fasting glucose levels. Results: Compared with the normal pregnant women, adiponectin was significantly lowered ［(10.3±2.4)ug/ml vs（15.6±3.2）μg/ml］, TNFα was elevated ［(4.6±1.5)ng/ml vs（3.3±1.6）pg/ml］, and leptin significantly highered ［(15.3±3.8)ng/ml vs （ 10.3±2.1）ng/ml ］ in women with pregnancy induced diabetes; TNFα and leptin levels positively correlated with serum insulin levels, glucose levels, Cpeptide and HbA1c, whereas adiponectin negtively with the corresponding parameters. Conclusions: ⑴ Women with pregnancy induced diabetes have significantly lower adiponectin and higher TNFα and leptin in comparison to normal pregnant women;⑵ Elevated TNFα, leptin and decreased adiponectin may not simply reflect maternal insulin resistant state, but contribute to the impaired glucose metabolism during pregnancy and increase risk for the development of type 2 diabetes. Adipocytokins may forecast the risk of metabolism syndrome.

Bone alkaline phosphatase measurement and its relation with osteosarcoma

YANG Zhi-ping,HUO Yan-qing,SUN Guang-zhi,LI Jian-min,LI Xin

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(6):  610-613. 

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To explore the value of bone alkaline phosphatase (BALP) on diagnosing osteosarcoma, evaluating the effect of chemotherapy, judging the prognosis and supervising the relapse and metastasis. Methods: The immunoassay was used to check the serum BALP in 42 patients with primary osteosarcoma, and auto biochemistry equipment to determine the serum Alkaline Phosphatase (ALP). The biopsy tissue and the lesion moved in the operation were treated with pathology, and their histological responses were counted. Results: During a followup from 5 months to 49 months with an average of 24.3 months, 18 patients had relapsed or transferred osteosarcoma, 16 died, others were surviving to the end of followup. BALP was more sensitive than ALP in diagnosing osteosarcomaConclusion: As a check mark in blood serum, BALP is more sensitive to the process of ossification than ALP, and therefore it is valuable in the diagnosis of osteosarcoma, reflection of the effect of chemotherapy and forecast of the prognosis.

Expression of IL10 in gingival lesion in adult periodontitis

CHENG Pei-hong,QI Xiang-min,SUN Shan-zhen

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(6):  622-625. 

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To investigate the expression of IL10 in gingival tissues of various clinical stages in adult periodontitis. Methods: Immunohistochemistry SABC method was used to semiquantitatively measure the expression of IL10 in 24 adults with periodontits(12 in periodontal abscess group and 12 in treatment group), 10 patients with gingivitis and 6 healthy controls. Results: The quantity of IL10 was lower in abscess group than that in healthy controls, gingivitis and treatment group, respectively (P＜0.05, P＜0.01, P＜0.01), and it was significantly higher in treatment group than that in healthy controls and gingivitis group (P＜0.01, P＜0.01). Conclusion: The expression of IL10 decreases in the activity of periodontitis, but increases after treatment, suggesting IL10 acts as a protective role in the restoration of periodontal tissues.

Evaluation destruction of ossicular chain in chronic otitismedia with MPR and MIP in HRCT

DING Yuan-ping,SUN Xiao-wei,LI Du-min,DENG Ji-bo

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(6):  630-633. 

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To observe the ossicular chain structure with multiplanar reconstruction (MPR) and maximum intensity projection (MIP) and evaluate role of MPR and MIP in the destruction of ossicular chain in chronic otitis media(COM). Methods：Highresolution CT scaning was performed in 20 patients (40 ears)with sudden deafness （control group ）and 62 patients (71 ears)with chronic otitis media(COM group) and the original data processed with MPR and MIP. The ossicular chain structure in the images generated by these techniques wasResults： In the control group, the ossicular chain structure was displayed in both MPR and MIP imaging,while MPR was superior to MIP on the structure of entire stape,and MIP was superior to MPR on the three dimentional structure of ossicular chain. In COM group,the destruction of ossicular chain was displayed by MPR and MIP respectively in 43 and 41 among the patients with cholesteatoma (44 ears), in 6 and 5 among the patients with granuloma (17 ears), and none among the patients with simple COM(10 ears);and it was displayed respectively in 41,6and 0 ear. Conclusion: Using MPR combined with MIP is helpful in the diagnosis and operation on the destruction of ossicular chain.

Application of three dimensional dynamic contrastenhanced MRA in abdomen and lower extremity arteries with 3.0T MR

MENG Xiang-shui,HOU Jin-wen,WEI Hua-gang,LI Chuan-fu,ZHAO Lei

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(6):  641-644. 

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To evaluate the three dimensional dynamic contrast enhanced MRA (3D DCEMRA) in demonstrating abdomen to lower extremity arteries with 3.0T MR. Methods： A total of 30 patients were detected by 3D DCEMRA with body coil and movebed technique using 3.0T MR of GE company, and they were injected gadolinium contrast medium through intravenous bolus with the Spectris MR injector of MEDRAD. All the primary graphs were analyzed the MIP and REFORMAT reconstruction by ADW4.2 software. Results： All the 3D DCEMRA angiographies were clearly demonstrated, of them, 12 patients were found with arteriosclerosis obliterans, and the result was consistent with that of the operation. Conclusion： 3D DCEMRA is a new and efficient method in abdomen to lower extremity arteries imaging.

Factors influence the shunting normal pressure hydrocephalus

HAN Tao,QU Yuan-ming,MU Cheng-zhi,XU Guang-ming,WANG Guo-dong

JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES). 2006, 44(6):  645-647. 

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To evaluate the factors acted on the shunting normal pressure hydrocephalus (NPH). Methods： The efficacy of shunting was retrospectively analyzed according to 31 NPH patients′ symptoms, medical history, age and test of draining cerebrospinal fluid（CSF） with lumbar puncture. Results： Seventeen patients got better after test of draining CSF with lumbar puncture, no one died. The total response rate was 70.9%, and the response rate of patients with gait disturbance was 72.7%, of patients with amentia was 44.4%, of patients with urine incontinence was 40%. Conclusion： Young patients with gait disturbance and short course of disease have good or excellent response to surgery, whereas patients with amentia or urine incontinence have poor response to surgery.