胃癌间充质干细胞与SGC-7901荷瘤鼠肿瘤进展的相关性

doi:10.6040/j.issn.1671-7554.0.2019.586

摘要/Abstract

摘要： 目的探讨在活体动物体内人胃癌组织间充质干细胞(hGC-MSCs)对SGC-7901胃癌荷瘤鼠模型肿瘤进展的作用。方法将实验鼠分为对照组hGC-MSCs组、实验组SGC-7901组和 hGC-MSCs+SGC-7901组。分别将相应的细胞悬液接种于实验鼠右侧背部皮下,观察肿瘤是否发生,肿瘤发生时间及肿瘤大小的变化;从不同的层面及角度,依次采用免疫组织化学染色、实时聚合酶链反应、蛋白质印迹法检测基质金属蛋白酶和增殖细胞核抗原在各实验鼠组中的表达水平。结果对照组中没有发生肿瘤,而实验组中hGC-MSCs+SGC-7901组与SGC-7901组相比,肿瘤发生时间短、生长速度快及肿瘤体积大,差异有统计学意义(P<0.05)。免疫组织化学染色结果显示增殖细胞核抗原在SGC-7901组和hGC-MSCs+SGC-7901组中的平均吸光度值分别为0.358±0.009、0.481±0.030,差异有统计学意义(P<0.05),基质金属蛋白酶-9在两组中的平均吸光度值分别为0.359±0.033、0.520±0.038,差异有统计学意义(P<0.05);实时聚合酶链反应证实hGC-MSCs能够上调PCNA、MMP-9的表达(P<0.01);Western blotting检测显示增殖细胞核抗原在两组中蛋白条带灰度值分别为0.756±0.039、0.847±0.048,差异有统计学意义(P<0.05)。基质金属蛋白酶-9在两组中蛋白条带灰度值分别为0.692±0.079、0.866±0.010,差异有统计学意义(P<0.05)。结论 hGC-MSCs在体内通过上调增殖细胞核抗原和基质金属蛋白酶-9的表达水平,从而参与肿瘤的发生及发展。

关键词: 胃癌, 间充质干细胞, 增殖细胞核抗原, 基质金属蛋白酶

Abstract: Objective To investigate the effects of human gastric cancer mesenchymal stem cells(hGC-MSCs)on the occurrence and development of gastric cancer cell line SGC-7901 in vivo. Methods The nude mice were divided into hGC-MSCs group, SGC-7901 group and hGC-MSCs+SGC-7901 group. The cell suspension was inoculated subcutaneously on the right back of the experimental mice to observe the occurrence of tumor, time of occurrence and change of tumor size. The expression levels of proliferating cell nuclear antigen(PCNA)and matrix metalloproteinase-9(MMP-9)were detected with immunohistochemical staining, real-time polymerase chain reaction(RT-PCR)and Western blotting. Results No tumor occurred in the control group. The hGC-MSCs+SGC-7901 group had shorter time of tumor occurrence, faster tumor growth and larger tumor size than the SGC-7901 group (P<0.05). The immunohistochemistry results showed that the average optical density of PCNA in SGC-7901 group and hGC-MSCs+SGC-7901 group was 0.358±0.009 and 0.481±0.030, respectively; the average optical density of MMP-9 in these two groups was 0.359±0.033 and 0.520±0.038, respectively (P<0.05). Real-time PCR demonstrated that hGC-MSCs could upregulate 山东大学学报 (医学版)57卷9期 -赵鸿渐,等.胃癌间充质干细胞与SGC-7901荷瘤鼠肿瘤荷瘤鼠肿瘤进展的相关性研究 \=-the expressions of PCNA and MMP-9 (P<0.01). Western blotting showed that the PCNA protein band grayscale in SGC-7901 group and hGC-MSCs+SGC-7901 group was 0.756±0.039 and 0.847±0.048, respectively; the MMP-9 protein band grayscale in these two groups was 0.692±0.079 and 0.866±0.010, respectively (P<0.05). Conclusion HGC-MSCs participate in the occurrence and development of tumors by up-regulating the expression levels of PCNA and MMP-9 in vivo.

Key words: Gastric cancer, Mesenchymal stem cells, Proliferating cell nuclear antigen, Matrix metalloproteinase

中图分类号:

R574

赵鸿渐,周继军,苏庆亮,赵硕,李玉明. 胃癌间充质干细胞与SGC-7901荷瘤鼠肿瘤进展的相关性[J]. 山东大学学报 (医学版), 2019, 57(9): 69-73.

ZHAO Hongjian, ZHOU Jijun, SU Qingliang, ZHAO Shuo, LI Yuming. Correlation between human gastric cancer mesenchymal stem cells and tumor progression in SGC-7901 tumor-bearing mice[J]. Journal of Shandong University (Health Sciences), 2019, 57(9): 69-73.

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