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山东大学学报（医学版） ›› 2015, Vol. 53 ›› Issue (1): 73-76.doi: 10.6040/j.issn.1671-7554.0.2014.600

• 临床医学 • 上一篇    下一篇

肾癌尿沉渣中人端粒酶逆转录酶基因启动子区甲基化检测的临床意义

任洪波, 刘海南, 刘承, 颜克强, 夏传友, 范医东   

  1. 山东大学齐鲁医院泌尿外科, 山东 济南 250012
  • 收稿日期:2014-09-11 修回日期:2014-11-25 发布日期:2015-01-10
  • 通讯作者: 范医东。E-mail:fanyd@sdu.edu.cn E-mail:fanyd@sdu.edu.cn
  • 基金资助:
    国家自然科学基金(81472395)

Clinical significance of human telomerase reverse transcriptase gene methylation status in urine sediments of renal cell carcinoma

REN Hongbo, LIU Hainan, LIU Cheng, YAN Keqiang, XIA Chuanyou, FAN Yidong   

  1. Department of Urology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Received:2014-09-11 Revised:2014-11-25 Published:2015-01-10

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摘要： 目的 探讨人端粒酶逆转录酶(hTERT)启动子区在肾癌患者尿沉渣中DNA甲基化状态及临床意义。方法 提取76例肾癌(肾癌组)和38例非肾癌(对照组)患者术前尿沉渣中的DNA,经亚硫酸氢钠修饰后,应用甲基化特异性PCR(MSP)方法对DNA进行扩增,将MSP产物进行凝胶电泳观察分析甲基化情况。结果 肾癌组患者hTERT启动子区高甲基化发生率明显高于对照组(P<0.05), 但hTERT启动子区高甲基化与性别、年龄、肿瘤大小、肿瘤分期无统计学关联(P>0.05)。结论 尿沉渣中hTERT启动子区甲基化与肾癌发生相关,并且其作为一种无创性检测有助于肾癌的临床诊断。

关键词: 尿沉渣, 甲基化, 人端粒酶逆转录酶, 肾肿瘤

Abstract: Objective To elucidate the methylation status of human telomerase reverse transcriptase (hTERT) promoter in urine sediments obtained from preoperative patients with renal cell carcinoma (RCC). Methods Genomic DNA was extracted from urine sediments of 76 RCC patients (RCC group) and 38 non-renal cell carcinoma patients (control group). Specimen DNA was modified with sodium bisulfite, methylation specific PCR (MSP) was carried out, and agar gel electrophoresis was performed to analyze the methylation status of hTERT gene. Results The rate of hTERT hypermethylation in RCC group was obviously higher than that in control group (P< 0.05). But no statistical correlation was observed between hTERT promoter methylation and gender, age, tumor size or clinical stage (P>0.05). Conclusion hTERT promoter in urine sediment of renal cell carcinoma patients is highly methylated, and as a noninvasive investigation, it might be useful in the clinical diagnosis of renal cell carcinoma.

Key words: Renal neoplasms, Methylation, Human telomerase reverse transcriptase, Urine sediments

中图分类号: 

  • R737.11
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