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山东大学学报（医学版）

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CXCL16和ox-LDL在阿霉素肾病小鼠中的变化及意义

许艺怀1，孙书珍1，甄军晖2，李倩1，王聪1   

  1. 1. 山东大学附属省立医院儿科肾脏风湿免疫科， 山东 济南 250021；
      2. 山东大学医学院病理学教研室， 山东 济南 250012
  • 收稿日期:2013-12-03 出版日期:2014-05-10 发布日期:2014-05-10
  • 通讯作者: 孙书珍 E-mail:ssztml@163.com
  • 基金资助:
    山东省自然科学基金（ZR2010HM110）

Roles of CXCL16 and ox-LDL in mice with adriamycin-induced nephropathy

XU Yihuai1, SUN Shuzhen1, ZHEN Junhui2, LI Qian1, WANG Cong1   

  1. 1. Department of Pediatric Nephrology and Rheumatology, Shandong Provincial Hospital Affiliated to Shandong University,
    Jinan 250021, Shandong, China;
    2. Department of Pathology, School of Medicine, Shandong University, Jinan 250012, Shandong, China
  • Received:2013-12-03 Online:2014-05-10 Published:2014-05-10

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摘要： 目的  观察CXCL16和氧化低密度脂蛋白（ox-LDL）在阿霉素肾病小鼠血、尿及肾组织中的变化， 探讨其与阿霉素肾病发生发展的关系。方法  将50只雄性Balb/c小鼠分为模型组与对照组。模型组予以一次性尾静脉注射阿霉素10.5mg/kg建模，对照组经尾静脉注射同样剂量的生理盐水。分别于实验0、1、3、5、7周末测24h尿蛋白定量，全自动生化分析仪检测血清白蛋白、胆固醇，双抗体夹心ELISA方法  检测血清CXCL16、ox-LDL及尿液CXCL16，免疫组织化学SABC法检测肾组织CXCL16及ox-LDL表达，PAS染色观察肾脏组织学改变。结果  与对照组相比，模型组小鼠1周末出现血清白蛋白降低(P＜0.05)，血清胆固醇及24h尿蛋白定量升高(P＜0.05或P＜0.01)，且持续至实验结束；模型组小鼠血尿CXCL16及血清ox-LDL水平明显增高(P＜0.01)，且随着时间延长逐渐增高；模型组肾小球CXCL16、ox-LDL表达较对照组增强(P＜0.01)，且随着时间延长表达逐渐增强。结论  CXCL16和ox-LDL均参与了阿霉素肾病的发生发展，且与疾病的严重程度有关。血清和尿液CXCL16水平可作为评价其肾组织病理损伤严重程度的无创性指标。

关键词: 肾病, 氧化低密度脂蛋白, CXCL16, 阿霉素, Balb/c小鼠

Abstract: Objective  To explore the roles of CXCL16 and oxygenized low density lipoprotein (ox-LDL) level in mice model with adriamycin-induced nephropathy. Methods  Fifty male Balb/c mice were separated into the model group and the control group. Mice in the model group were intravenously injected with a single dose of adriamycin (10.5mg/kg), and those in the control group were injuected with the same dosage of physiological saline. Determined 24h urinary protein were observed at the end of the 0, 1st, 3rd, 5th and 7th weeks; serum and cholesterol were determined by automatic biochemical analyzer; serum CXCL16,  ox-LDL and urinary CXCL16 were determined by ELISA; the expressions of kidney CXCL16 and ox-LDL were detected by semiquantitative immunohistochemistry, and pathology were observed by light and electron microscopy. Results  Decreased serum albumin(P＜0.05) and increased proteinuria(P＜0.01) and serum cholesterol(P＜0.05) levels were observed in the model group compared with those in the control group. CXCL16 levels in serum, urine and renal tissue and ox-LDL levels in serum and renal tissue increased significantly in the model group, compared with those in the control group(P＜0.01). And it kept insistent increase for the model mice as time went on. CXCL16 and ox-LDL protein expressions in kidneys in the model group were strengthed remarkably, compared with those in the control group(P＜0.01). Conclusion  CXCL 16  and ox-LDL might take part in the progress of adriamycin-induced nephropathy and correlate with the severity of disease. Serum and urinary CXCL16 levels might be a non-invasive predictor to evaluate the severity of renal injury.

Key words: Nephropathy, Balb/c mice, CXCL16, Oxygenized low density lipoprotein, Adriamycin

中图分类号: 

  • R725.4
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