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山东大学学报（医学版） ›› 2017, Vol. 55 ›› Issue (7): 1-5.doi: 10.6040/j.issn.1671-7554.0.2016.1301

• 基础医学 •    下一篇

巴马小型猪冠状动脉粥样硬化模型的评价方法

尹妮1,杨关林1,姜钧文2,王春田3,王凤耀4,贾连群5,高晓宇1,潘嘉祥1,李芹1,李佳1,冯元洁1,高玉竹1,周鹤1,张哲1   

  1. 1.辽宁中医药大学附属医院血脉病研究室, 辽宁 沈阳 110032;2.辽宁中医药大学附属医院心内科, 辽宁 沈阳 110032;3.辽宁中医药大学实验动物中心, 辽宁 沈阳 110847;4.辽宁中医药大学附属医院麻醉科, 辽宁 沈阳 110032;5.辽宁中医药大学重大科研平台, 辽宁 沈阳 110847
  • 收稿日期:2016-10-13 出版日期:2017-07-10 发布日期:2017-07-10
  • 通讯作者: 张哲. E-mail:pedtrainzhzh7676@163.com E-mail:pedtrainzhzh7676@163.com
  • 基金资助:
    国家重点基础研究发展计划(2013CB531704)

A reliable system to assess atherosclerosis model of Bama minipigs

YIN Ni1, YANG Guanlin1, JIANG Junwen2, WANG Chuntian3, WANG Fengyao4, JIA Lianqun5, GAO Xiaoyu1, PAN Jiaxiang1, LI Qin1, LI Jia1, FENG Yuanjie1, GAO Yuzhu1, ZHOU He1, ZHANG Zhe1   

  1. 1. Blood-vessel Laboratory, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China;
    2. Department of Cardiology, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China;
    3. Laboratory Animal Center, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, Liaoning, China;
    4. Department of Anesthesia, Affiliated Hospital of Liaoning University of Traditional Chinese Medicine, Shenyang 110032, Liaoning, China;
    5. Platform of Scientific Research, Liaoning University of Traditional Chinese Medicine, Shenyang 110847, Liaoning, China
  • Received:2016-10-13 Online:2017-07-10 Published:2017-07-10

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摘要： 目的 从可行性、稳定性和安全性等方面对巴马小型猪冠状动脉粥样硬化模型进行评价。 方法 巴马小型猪随机分为对照组(给予基础饲料)和模型组(给予高脂喂饲联合机械损伤),在0、24、48周分别对两组行冠状动脉造影、血管内超声(IVUS)、心电图、血脂、炎症指标检测和病理学分析。 结果 24周模型组总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白(HDL-C)、氧化低密度脂蛋白(ox-LDL)、高敏C反应蛋白(hs-CRP)、白介素-6(IL-6)、血管细胞黏附分子-1(VCAM-1)、单核细胞趋化因子-1(MCP-1)和P选择素(sP-selectin)明显高于对照组(P<0.05);模型组0、24周ST段下移程度与对照组相比差异无统计学意义(P>0.05),模型组0周T波振幅与对照组相比差异无统计学意义(P>0.05),模型组24周T波振幅与对照组相比差异有统计学意义(P<0.05);24、48周冠状动脉造影和IVUS显示模型组冠脉平均面积狭窄率高于对照组;48周模型组血管内膜厚度和内膜中膜厚度比大于24周。 结论 离体在体结合评价是一种可行、安全的评价冠状动脉粥样硬化模型的方法。

关键词: 模型评价, 巴马小型猪, 动物模型, 机械损伤, 动脉粥样硬化, 高脂喂饲

Abstract: Objective To evaluate the feasibility, stability and safety of a coronary atherosclerosis model of Bama minipigs. Methods Bama minipigs were randomly assigned into control group(basic diet)and model group(high-fat diet combined with mechanical injury). In week 0, 24 and 48, both groups underwent coronary angiography, intravascular ultrasound(IVUS), electrocardiogram, serum lipid testing, inflammation factors testing, and pathological analysis 山 东 大 学 学 报 (医 学 版)55卷7期 -尹妮,等.巴马小型猪冠状动脉粥样硬化模型的评价方法 \=-to evaluate the model. Results In week 24, compared with the control group, the model group had significantly increased total cholesterol(TC), triglycerides(TG), low density lipoprotein cholesterol(LDL-C), high density lipoprotein cholesterol(HDL-C), oxidized low-density lipoprotein(ox-LDL), high-sensitivity C-reactive protein(hs-CRP), interleukin-6(IL-6), vascular cell adhesion molecule-1(VCAM-1), monocyte chemotactic protein(MCP-1)and soluble P-selectin(SP-selectin)(P<0.05). There was no statistically significant difference between the two groups in ST segment depression in week 0 and 24(P>0.05). There was no statistically significant difference between the two groups in T waves in week 0(P>0.05), but there was statistically significant difference in T waves in week 24(P<0.05). In week 24 and 48, the model group showed significantly higher coronary stenosis rate than the control group. In week 48, the model group had higher intima/intima-medial thickness ratio than in week 24. Conclusion The in vitro and in vivo model is safe and feasible in the assessment of atherosclerosis.

Key words: Atherosclerosis, Bama minipigs, High-fat diet, Model evaluation, Mechanical injury, Animal model

中图分类号: 

  • R332
[1] Zaragoza C, Gomez-Guerrero C, Martin-Ventura JL, et al. Animal models of cardiovascular diseases[J]. J Biomed Biotechnol, 2011, 2011: 497841. doi: 10.1155/2011/497841.
[2] McGill HC Jr, McMahan CA, Gidding SS. Preventing heart disease in the 21st century implications of the Pathobiological Determinants of Atherosclerosis in Youth(PDAY)study[J]. Circulation, 2008, 117(9): 1216-1227.
[3] 陈伟伟, 高润霖, 刘力生, 等. 《中国心血管病报告2014》概要[J]. 中国循环杂志, 2015, 7: 617-622.
[4] 韩雅玲. 中国经皮冠状动脉介入治疗指南2012(简本)[J]. 中华心血管病杂志, 2012, 40(4): 18-26.
[5] Xiangdong L, Yuanwu L, Hua Z, et al. Animal models for the atherosclerosis research: a review[J]. Protein Cell, 2011, 2(3): 189-201.
[6] Smith AC, Swindle MM. Preparation of swine for the laboratory[J]. ILAR J, 2006, 47(4): 358-363.
[7] Casellas J, Vidal O, Pena RN, et al. Genetics of serum and muscle lipids in pigs[J]. Anim Genet, 2013, 44(6): 609-619.
[8] Bergen WG, Mersmann HJ. Comparative aspects of lipid metabolism: impact on contemporary research and use of animal models[J]. J Nutr, 2005, 135(11): 2499-2502.
[9] Hammad SM, Siegel HS, Marks HL. Total cholesterol, total triglycerides, and cholesterol distribution among lipoproteins as predictors of atherosclerosis in selected lines of Japanese quail[J]. Comp Biochem Physiol A MolIntegr Physiol, 1998, 119(2): 485-492.
[10] Weiner BH, Ockene IS, Levine PH, et al. Inhibition of atherosclerosis by cod-liver oil in a hyperlipidemic swine model[J]. N Engl J Med, 1986, 315(14): 841-846.
[11] 黎洪展, 吕永恒, 陈琪, 等. 动脉粥样硬化大鼠主动脉内膜增生与树突状细胞分布的关系[J]. 临床心血管病杂志, 2011, 27(9): 655-657. LI Hongzhan, LÜ Yongheng, CHEN Qi, et al. Relationship of dendreiec cells distribution and intima hvperplasia in atherosclerotic mice[J]. Journal of Clinical Cardiology, 2011, 27(9): 655-657.
[12] Wang M, Gao M, Liao J, et al. Adipose tissue deficiency results in severe hyperlipidemia and atherosclerosis in the low-density lipoprotein receptor knockout mice[J]. Biochim Biophys Acta, 2016, 1861(5): 410-418.
[13] 张美玲, 王茁伉, 彭成. 动脉粥样硬化动物模型研究进展[J]. 四川动物, 2009, 28(5): 794-797.
[14] Gerrity RG, Natarajan R, Nadler JL, et al. Diabetes-induced accelerated atherosclerosis in swine[J]. Diabetes, 2001, 50(7): 1654-1665.
[15] Xu X, Mao W, Chai Y, et al. Angiogenesis inhibitor, endostar, prevents vasa vasorum neovascularization in a swine atherosclerosis model[J]. J Atheroscler Thromb, 2015, 22(10): 1100-1112.
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