Abstract: To research the potential ability of bone marrow stromal cells(BMSCs) to differentiate into osteoblasts and the influence of basic fibroblast growth factor(bFGF) on BMSCs adhesion, proliferation and differentiation. Methods：After the BMSCs were cultured successfully, 0, 5, 10, 50, 100, 200?ng/ml bFGF was given respectively. The BMSCs′morphology, adhesion, proliferation and the potentiality of differentiation and calcification in vitro were studied by contrast microscope, electron microscope, MTT test, ALP staining, calcium staining and electronic probe. Results: The adhesion of BMSCs remarkably increased after induced by bFGF at a concentration of 10?ng/ml,　and it reached the peak by bFGF at a concentration of 100?ng/ml. After 100?ng/ml bFGF was added into medium, BMSCs showed a lower positivity rate in ALP staining. After cultured 30 days in conditioned medium, the BMSCs showed apositivity result in Red alizarin calcium staining. The electronic probe result revealed that the main elements of the mineralized nodes were calcium and phosphorus, and this ratio of Ca to P corresponded with the ratio in HA. The cultured BMSCs showed typical osteoblast morphological features in SEM and TEM, in addition, collagen fiber was found in SEM. Conclusion: The BMSCs cultured in vitro can differentiate into osteoblasts. bFGF has a positive influence on BMSCs adhesion and proliferation and a negative influence on their differentiation.

Key words: Bone marrow stromal cells, Osteoblasts, Fibroblast growth factor 2, Rabbits