Journal of Shandong University (Health Sciences) ›› 2023, Vol. 61 ›› Issue (2): 78-87.doi: 10.6040/j.issn.1671-7554.0.2022.1273

• 临床医学 • Previous Articles     Next Articles

Effects of circular RNA hsa_circ_0008591 on tumor biological behavior of breast cancer cells

DONG Xiangjun1, LI Juan1, KONG Xue1, LI Peilong1, ZHAO Wenjing2, LIANG Yiran3, WANG Lili4, DU Lutao1, WANG Chuanxin1   

  1. 1. Department of Clinical Laboratory, The Second Hospital of Shandong University, Jinan 250033, Shandong, China;
    2. Pathology Tissue Bank, Department of Scientific Research, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    3. Department of Breast Surgery, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    4. Department of Clinical Laboratory, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China
  • Published:2023-02-17

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Abstract: Objective To investigate the expression of circular RNA(circRNA)hsa_circ_0008591 and its effects on the biological behavior of breast cancer(BC)cells. Methods The differentially expressed circRNAs in BC tissues were screened using high-throughput circRNA microarray, and hsa_circ_0008591 was selected as a candidate, which was characterized by Sanger sequencing and ribonuclease R(RNaseR)treatment. Quantitative real-time PCR(qRT-PCR)was performed to detect the expression of hsa_circ_0008591 in BC tissues and cells. Furthermore, BC cells were transfected with plasmid or small interfering RNA(siRNA)and the effects of hsa_circ_0008591 on cell proliferation were detected with real-time cellular analysis(RTCA), cell counting kit-8(CCK-8)assay, 5-ethynyl-2'-deoxyuridine(EdU)assay, and colony formation assay. The interacting microRNAs(miRNAs)and proteins were predicted by databases such as TargetScan, and function enrichment analysis were performed. A regulatory network of ceRNA was constructed with Cytoscape software. Results Hsa_circ_0008591 was significantly downregulated in BC tissues(P=0.007 6). Compared with normal breast epithelial cells, six BC cell lines had a lower expression of hsa_circ_0008591(P<0.001). In vitro functional experiments showed that overexpression of hsa_circ_0008591 inhibited the proliferation of MDA-MB-231(PRTCA<0.001, PEdU=0.000 6, Pcolony=0.001 0)and MCF-7 cells(PRTCA<0.001, PEdU=0.001 7, Pcolony<0.001), while the knockdown of hsa_circ_0008591 promoted the proliferation of SK-BR-3 cells(PRTCA<0.001, PEdU<0.001, Pcolony=0.003 4). The downstream targets, gene ontology(GO)analysis, and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment indicated that hsa_circ_0008591 inhibited the progression of BC through ceRNA mechanism or interaction with RNA-binding protein(RBP). Conclusion Circular RNA hsa_circ_0008591 can inhibit the proliferation of BC cells and is expected to be an effective intervention target for the treatment of BC.

Key words: Circular RNA, Hsa_circ_0008591, Breast cancer, Proliferation, Biological behavior

CLC Number: 

  • R737.9
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