Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (7): 72-79.doi: 10.6040/j.issn.1671-7554.0.2019.002

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Changes of intestinal microbiota in patients with chronic kidney disease 5

LÜ Chenxiao1, LI Yang2, GAO Ying1, ZHANG Qunye3, ZHANG Lei4, WANG Zunsong2   

  1. 1. Weifang Medical University, Weifang 261053, Shandong, China;
    2. Department of Nephrology, the First Hospital Affiliated with Shandong First Medical Uriversity, Shandong Provincial Qianfoshan Hospital, Jinan 250014, Shandong, China;
    3. Department of Cardiology, Qilu Hospital of Shandong University, Jinan 250012, Shandong, China;
    4. School of Chemistry and Environment, Beihang University, Beijing 100191, China
  • Published:2022-09-27

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Abstract: Objective To explore the differences of gut microbiota composition between chronic kidney disease 5(CKD5)patients and healthy people. Methods The individuals were divided into the healthy control group(n=69), CKD5-none hemodialysis(NHD)group(n=24)and CKD5-hemodialysis(HD)group(n=29). 16S rRNA gene sequencing technology was used to analyze differences of the gut microbiota composition among the three groups. LDA>2.0 indicated significant differences in the biome between two groups. Based on the sequencing results, the function of intestinal microflora in CKD5 patients was studied by means of STAMP software mapping to KEGG and COG database. Results In the healthy control group, Bacteroidetes, Gammaproteobacteria, and Enterobacteriaceae were dominant. Enterococcaceae, Eubacterium, Rhodobacteraceae were high in CKD5-HD group, while Actinobacteria, Bifidobacterium, Coriobacteriales were dominant in the CKD5-NHD group. In the CKD5 patients, glycerolipid, carbohydrate, protein and amino acid metabolism activity was increased. Conclusion Significant differences were found in the composition and function of the gut microbiota between the CKD5 patients and healthy individuals. Imbalance of the gut micro- 山 东 大 学 学 报 (医 学 版)57卷7期 -吕晨箫,等. 慢性肾脏病5期患者的肠道菌群变化 \=-biota in CKD5 patients may increased the risk of complications by influencing a variety of metabolic and signaling pathways.

Key words: Chronic kidney disease, Hemodialysis, None hemodialysis, Intestinal microbiota, Microbial 16S rDNA sequencing

CLC Number: 

  • R574
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