Journal of Shandong University (Health Sciences) ›› 2019, Vol. 57 ›› Issue (3): 31-37.doi: 10.6040/j.issn.1671-7554.0.2018.763

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Establishment of sensitization model based on the activation of basophil in mice

GUO Yanru1, SUN Rong2, LI Wei1, LIU Zhaohua1   

  1. 1. Center for New Drug Evaluation School, Pharmaceutical Sciences of Shandong University, Jinan 250012, Shandong, China;
    2. Shandong Academy of Chinese Medicine, Jinan 250014, Shandong, China
  • Published:2022-09-27

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Abstract: Objective To detect the expression of CD63 of basophil in the peripheral blood of sensitized BALB/c mice, and to establish the basophils activation test(BAT)for assessing the sensitization of low molecule weight compounds(LMWC). Methods The morphology and number of basophils in control BALB/c mice(control group)and ovalbumin(OVA)-sensitized BALB/c mice(OVA-sensitized group)were determined by flow cytometry(CD45 APClow/IgE FITChigh)and peripheral blood smears stained by Wright-Giemsa. CD45 APClow/IgE FITChigh was used as the flow scheme to identify mice basophils, and anti-IgE was used as a positive control to incubate with basophils in vitro to observe the expression of CD63 and establish the BAT of mice. In addition, the BALB/c mice was sensitized with penicillin G conjugated BSA(PG/BSA group), and the blood was stimulated by penicillin G-conjugated OVA(PG/OVA)in vitro to observe the expression of CD63, thereby detect the effectiveness of BAT. Results Flow cytometry and blood smears showed that the morphology and numbers of basophils in control group and OVA-sensitized 山 东 大 学 学 报 (医 学 版)57卷3期 -郭岩乳,等.基于小鼠嗜碱性粒细胞活化致敏性模型的建立 \=-group had no significant difference(P=0.655,P=0.667). Anti-IgE could stimulate the expression of CD63 on basophils. After the incubation with OVA in vitro, the CD63 expression in peripheral blood of the OVA-sensitized group was higher than the control group［(59.70±37.93)% vs (2.15±1.13)%, P=0.016］. After the incubation with PG/BSA in vitro, the CD63 expression in peripheral blood of the PG/BSA-sensitized group was higher than the control group［(62.74±41.05)% vs (2.73±1.40)%,P=0.019］. Conclusion Specific allergen in vitro can lead to increased CD63 expression in the sensitized mice, indicating the successful construction of BAT in mice, which is effective verified by PG and can be used to evaluate the sensitization of LMWC.

Key words: Basophils, Basophil activation test, CD63, Low molecule weight compounds, Drug type Ⅰ hypersensitivity

CLC Number: 

  • R619
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