JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (9): 41-45.doi: 10.6040/j.issn.1671-7554.0.2016.1656

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Improvement of pediocin yield by a novel high residual sugar and re-alkalized feeding strategy

QIN Lumin, SUN Aiyou, WANG Qianwen, GAO Jingwen, WEI Dongzhi   

  1. State Key Laboratoty of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
  • Received:2016-12-14 Online:2017-09-10 Published:2017-09-10

Abstract: Objective To improve the pediocin yield by designing a novel high residual sugar and re-alkalized feeding(F4)strategy. Methods (1) The initial glucose concentration was 8.8 g/L in the first phase; (2) When the residual glucose concentration was lower than 2 g/L, the second phase started, and the glucose concentration kept about 20 g/L in the whole second phase; at the same time, the pH values of fermentation broth were raised to 6.5 every 6 h, which make the pH value re-alkalize repeatedly. Results The max titer of pediocin reached 24 000 AU/mL in the optimized feeding strategy F4. The pediocin yield of F4 strategy is 3, 3, and 1.7 times of F1, F2 and F3 strategies. Conclusion F4 strategy can not only increase the cell concentration, but also improve the yield of pediocin, which is suitable for mass production of pediocin.

Key words: Glucose with high concentration, Pediocin, pH re-alkalization shock, Feeding strategy, Fermentation

CLC Number: 

  • R979.7
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