JOURNAL OF SHANDONG UNIVERSITY (HEALTH SCIENCES) ›› 2017, Vol. 55 ›› Issue (8): 42-47.doi: 10.6040/j.issn.1671-7554.0.2017.339

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Preparation and characterization of PEG coating levofloxacin-liposomes

ZHANG Xinke1, CHEN Ying2, LI Peng3, CHEN Hongyuan4, HUANG Guihua5   

  1. 1. Drug Screening Platform, School of Pharmaceutical Science, Shandong University, Jinan 250012, Shandong, China;
    2. Department of Gynaecology and Obstetrics, Rizhao Municipal Peoples Hospital, Rizhao 276826, Shandong, China;
    3. Shandong Academy of Environmental Science, Jinan 250013, Shandong, China;
    4. Department of General Surgery, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong, China;
    5.Institute of Pharmaceutics, School of Pharmaceutical Science, Shandong University, Jinan 250012, Shandong, China
  • Received:2017-04-19 Online:2017-08-10 Published:2017-08-10

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Abstract: Objective PEG2000 was used to coat levofloxacin-liposomes(Lvf-lips)in order to improve the stability. The physico-chemical property, preliminary stability and pharmacokinetics of PEG 2000 coating levofloxacin-liposome(PEG Lvf-lips)were systematically investigated. Methods Lvf-lips were prepared by ammonium sulfate gradients method, and the optimal preparation technique of PEG Lvf-lips was screened based on the entrapment efficiency and preliminary stability. The properties of PEG Lvf-lips, such as morphology, diameter and size distribution, were observed with transmission electric microscope, laser dispersive analyzing and micro-electrophoresis apparatus. In vitro drug release of PEG Lvf-lips was performed with the dialysis bag diffusion technique. The pharmacokinetics property was also studied. Results PEG Lvf-lips had spherical shapes and uniform particle size. The entrapment efficiency was(77.97±1.09)%. The stability and sustained release effects in vitro and in vivo PEG Lvf-lips were superior to non-PEG coating liposomes. Conclusion PEG 2000 coating can significantly improve the stability of Lvf-lips, which provides a new pathway for liposome stability.

Key words: Levofloxacin, Pulmonary targeted liposome, Ammonium sulfate gradients method, PEG coating, Stability

CLC Number: 

  • R944.9
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